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RNASeqData
•
reset
0
votes
1
reply
106
views
Correlation between gene clusters
genomes
RNASeqData
written 14 days ago by
Le
• 0
0
votes
2
replies
210
views
Mean variance curve troubleshoot filtering
limmavoom
filtering
edgeR
RNASeqData
meanvariancetrend
15 days ago • updated 14 days ago
gingergeiger22
• 0
2
votes
1
reply
337
views
Is it possible to work with DESeq2 where data is not properly matched for sex and age
DESeq2
RNASeqData
updated 16 days ago by
Michael Love
43k • written 24 days ago by
KR
• 0
0
votes
1
reply
173
views
combining raw counts and estimated counts
RNASeqData
GEO
TCGAbiolinks
updated 25 days ago by
O'Brien
• 0 • written 27 days ago by
Noor
• 0
1
vote
1
reply
278
views
DESeq2- merging technical vs biological replicates
DESeq2
RNASeqData
updated 6 weeks ago by
Michael Love
43k • written 8 weeks ago by
Nikita
• 0
0
votes
4
replies
657
views
RNA-seq: samples form different experiments, how to do DGEs?
DGEs
edgeR
RNASeqData
12 weeks ago
Philippine
• 0
0
votes
2
replies
375
views
ESTMATE, MCPcounter, EPIC, xCell input
DESeq2
ESTIMATE
RNASeqData
MCPcounter
written 3 months ago by
Aleksandra
• 0
3
votes
11
replies
816
views
Highly DE genes but small count difference within conditions
RNASeq
DESeq2
RNASeqData
written 3 months ago by
bioshot.com
• 0
1
vote
1
reply
408
views
WGCNA
DEGselection
RNASeqData
WGCNA
updated 3 months ago by
Lluís Revilla Sancho
▴ 760 • written 3 months ago by
Nasim
• 0
1
vote
1
reply
282
views
Normalization RNA-seq for multiple plates
Normalization
DESeq2
RNASeqData
edgeR
updated 3 months ago by
ATpoint
★ 4.6k • written 3 months ago by
Pimpa
• 0
1
vote
1
reply
492
views
Help with design matrix for RNA-seq data using edgeR
edgeR
RNASeqData
updated 4 months ago by
Gordon Smyth
52k • written 4 months ago by
erik.burchard
• 0
1
vote
2
replies
1.3k
views
How to use compareCluster in ClusterProfiler?
RNASeqData
GSEA
clusterProfiler
written 4 months ago by
b8177
• 0
1
vote
2
replies
360
views
Questions in Regards to LFC shrinkage in DESeq2
Dseq2
RNASeqData
apeglm
4 months ago
Gordon
• 0
0
votes
3
replies
614
views
Error when trying to do different pairwise comparisons and lfcshrink than what's listed on resultsNames()
RNASeqData
DESeq2
RNASeq
DifferentialExpression
updated 6 months ago by
ATpoint
★ 4.6k • written 6 months ago by
Mark
• 0
0
votes
4
replies
636
views
DE analysis in EdgeR /Can any one please help me.
RNASeqData
DEGraph
RNASeq
edgeR
DegNorm
written 6 months ago by
Shaozhen
• 0
1
vote
3
replies
1.1k
views
Combining two bulk RNA seq datasets
RNASeqData
DESeq2
Normalization
7 months ago
Tanvi
• 0
1
vote
3
replies
617
views
Including infectivity information to RNA-Seq analysis
RNASeqData
DESeq2
RNA-Seq
RNASeq
updated 7 months ago by
James W. MacDonald
67k • written 7 months ago by
Ben
▴ 50
0
votes
1
reply
391
views
How to identify transcripts/genes with differential kinetics in two genotypes, using time-course RNA seq data?
RNASeqData
DESeq2
DifferentialExpression
updated 7 months ago by
James W. MacDonald
67k • written 7 months ago by
ZhuYi
• 0
0
votes
1
reply
396
views
ReactomeGSA for RNASeq
ReactomeGSA
BatchEffect
RNASeqData
updated 4 months ago by
jg
▴ 20 • written 8 months ago by
Shaimaa Gamal
• 0
0
votes
1
reply
416
views
NEED FOR NESSASORY HELP
RNASeqData
edgeR
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
z_bahari
• 0
0
votes
1
reply
354
views
Brachypodium distachyon db
EnrichmentBrowser
BrachypodiumDB
RNASeqData
updated 9 months ago by
James W. MacDonald
67k • written 9 months ago by
Leyre
• 0
3
votes
5
replies
1.2k
views
DESEQ Inquiry
DESeq2
rnaseqGene
RNASeqData
limma
RNASeq
updated 10 months ago by
Michael Love
43k • written 11 months ago by
sap275
▴ 10
0
votes
0
replies
540
views
Differential expression analysis between different species
DEG
RNASeqData
DifferentialExpression
SCBN
RNASeq
11 months ago
Abril
• 0
1
vote
2
replies
1.9k
views
How to combine a bulk and pseudobulk dataset?
DESeq2
limma
RNASeq
RNASeqData
written 20 months ago by
informatician
• 0
1
vote
3
replies
737
views
DESeq2 analysis on time-series RNA experiment
RNASeqData
DESeq2
RNASeq
11 months ago
island
• 0
0
votes
2
replies
541
views
ConsensusClusterPlus dosesn't make an output file
ConsensusClusterPlus
RNASeqData
12 months ago • updated 11 months ago
Diana
• 0
0
votes
1
reply
787
views
WGCNA Soft Power
Normalization
DESeq2
Network
RNASeqData
updated 12 months ago by
ATpoint
★ 4.6k • written 12 months ago by
Ömür Koray
• 0
5
votes
6
replies
1.0k
views
Monotonic genes in results of differential gene expression analysis
DifferentialExpression
rnaseqGene
RNASeqData
12 months ago
Shaimaa Gamal
• 0
1
vote
1
reply
681
views
Deseq2 -Model matrix not full rank error: A complex case
RNASeqData
DifferentialExpression
DESeq2
updated 13 months ago by
ATpoint
★ 4.6k • written 13 months ago by
kubracelikbas4
• 0
0
votes
2
replies
788
views
StringDB: Cannot use payload mechanism to colour nodes
RNASeqData
STRINGdb
DifferentialExpression
GraphAndNetwork
Network
13 months ago
Luca
• 0
0
votes
1
reply
1.1k
views
Interaction terms in DESeq2
RNASeqData
RnaSeqSampleSize
DESeq2
updated 13 months ago by
James W. MacDonald
67k • written 13 months ago by
Mimi
• 0
1
vote
2
replies
809
views
RNASeq time course analysis, missing time points for control
RNASeqData
RnaSeqSampleSize
RNASeq
RNASeqPower
13 months ago
drewhaydensteele
• 0
1
vote
1
reply
667
views
Differential gene expression analysis
RNASeqData
RNAseq123
updated 13 months ago by
ATpoint
★ 4.6k • written 13 months ago by
kaurprab
• 0
1
vote
2
replies
1.3k
views
Different results from blockwiseModules and blockwiseConsensusModules individual TOM
RNASeqData
WGCNA
consensusnetworks
13 months ago
Levi
• 0
0
votes
3
replies
1.7k
views
DESeq: Error in DESeqDataSet(se, design = design, ignoreRank) : some values in assay are negative
RNASeqData
DESeq2
13 months ago
Oenone
• 0
3
votes
4
replies
1.0k
views
Is it appropriate to apply linear mixed models to Voom transformed data?
stats
r
RNASeqData
limma
voom
14 months ago
pl23
• 0
0
votes
1
reply
564
views
DESeq2 and determining effects of treatment on a priori/candidate genes from a bulk-RNAseq experiment
RNASeqData
DifferentialExpression
DESeq2
updated 14 months ago by
Michael Love
43k • written 14 months ago by
Pez
▴ 10
2
votes
2
replies
731
views
DESeq2: Comparison of single clinical sample to 4 normals using tumour cohort to infer dispersion of single sample
GeneExpression
DESeq2
RNASeqData
RNASeq
StatisticalMethod
14 months ago
Manthos
• 0
0
votes
1
reply
670
views
DESeq2 interaction between one continuous variable and one categorical variable
RNASeqData
DESeq2
updated 14 months ago by
Wolfgang Huber
★ 13k • written 14 months ago by
meroy
• 0
0
votes
2
replies
717
views
Is my data too noisy for DESeq? Should I model noise as unwanted variation?
RNASeqData
DESeq2
14 months ago
Ben
▴ 10
0
votes
2
replies
1.8k
views
MA plot for DESEq2 analysis
RNASeqData
DESeq2
14 months ago
RHEA
• 0
2
votes
3
replies
1.8k
views
Two factor design including interaction effect with DESeq2 in R
DESeq2
RNASeqData
deseq2
ExperimentalDesign
updated 15 months ago by
swbarnes2
★ 1.4k • written 15 months ago by
Sam
• 0
1
vote
1
reply
760
views
Multiple comparison using DESeq2
RNASeqData
RNASeq
DESeq2
updated 15 months ago by
James W. MacDonald
67k • written 15 months ago by
fuqichen1
• 0
1
vote
7
replies
1.3k
views
DuplicateCorrelation() block by duplicate sample or by cell line ?
variancePartition
RNASeqData
limma
BulkRNAseq
RNASeq
updated 13 months ago by
Gordon Smyth
52k • written 15 months ago by
Gimena
• 0
0
votes
1
reply
704
views
Comparing RNASeq data from multiple studies
RNASeqData
RnaSeqSampleSize
RNASeqPower
RNASeq
updated 15 months ago by
Basti
▴ 780 • written 15 months ago by
Luke
• 0
2
votes
4
replies
1.2k
views
Deseq2 calculated log2foldchange not consistent with RPM in IP-small RNA-seq
RNASeqData
DifferentialExpression
SmallRNAData
DESeq2
16 months ago • updated 15 months ago
Shihui
• 0
0
votes
4
replies
1.1k
views
filtering out lowly expressed genes by groups
RNASeqData
DESeq2
updated 16 months ago by
James W. MacDonald
67k • written 16 months ago by
sohail.mbio
▴ 10
3
votes
3
replies
2.6k
views
Differential expression analysis with only FPKM matrix available from total newbie in R
FPKM
RNASeqData
DEAnalysis
limma
16 months ago
Ethan Nguyen
• 0
1
vote
1
reply
1.2k
views
Can I transform already normalized rna-seq data with Z-score?
Z-score
RNASeqData
Deseq
DESeq2
updated 16 months ago by
James W. MacDonald
67k • written 16 months ago by
khaserdene99
• 0
0
votes
2
replies
853
views
RSubread DESeq2
RNASeqData
DifferentialExpression
DESeq2
16 months ago
Beyza
• 0
50 results • Page
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Recent ...
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Comment: How to sort negative log2FC or FC in RNAseq for GSEA?
by
QN
▴ 10
Thank you ATpoint for your response! In the result of GSEA, like you indicated, there will be gene sets that have positive NES and gene se…
Comment: EdgeR Biological CVs
by
ATpoint
★ 4.6k
"Joseph" is a spam bot...
Answer: How to sort negative log2FC or FC in RNAseq for GSEA?
by
ATpoint
★ 4.6k
GSEA asks whether there are general shifts in gene expression towards certain terms. Hence, you would not split your results tables but ord…
Comment: EdgeR Biological CVs
by
James
• 0
Thank you Gordon and Joseph. I presume question (1) would be answered by using a prior G0 of 0? For question (2), I was expecting a biolog…
Comment: EdgeR Biological CVs
by
Joseph
▴ 10
I believe the published study provides answers to your questions. Yunshun Chen and Pedro Baldoni's recent papers on edgeR may also be of in…
Votes
How to correct for tumor purity using DESeq2 to analyze TCGA tumor sample expression.
Answer: EdgeR Biological CVs
Comment: EdgeR Biological CVs
Answer: Error in download.file for TCGAutils::symbolsToRanges
Answer: Error in download.file for TCGAutils::symbolsToRanges
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