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GeneExpression
•
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0
votes
1
reply
173
views
WGCNA sample-data preprocessing order
Microarray
WGCNA
GeneExpression
updated 28 days ago by
ATpoint
★ 4.7k • written 4 weeks ago by
Reuben
• 0
2
votes
1
reply
263
views
Using Affymetrix Custom Array
ArrayExpress
makecdfenv
AffymetrixChip
gcrma
GeneExpression
updated 3 months ago by
James W. MacDonald
68k • written 3 months ago by
Piotr.Keller
• 0
0
votes
1
reply
301
views
Is my time course analysis with DESeq2 valid?
GeneExpression
DESeq2
TimeCourse
updated 3 months ago by
Michael Love
43k • written 3 months ago by
Jason
• 0
4
votes
9
replies
921
views
Can DESeq2 rlog normalized matrix reflects time course result directly?
DESeq2
timecoursedata
GeneExpression
updated 9 months ago by
ATpoint
★ 4.7k • written 9 months ago by
pangtaihin
• 0
2
votes
3
replies
790
views
"could not find function "readTSGE" in package ctsGE" Error occurs when trying to follow ctsGE package instructions.
GeneExpression
Clustering
ctsGE
11 months ago
jbgnielson
• 0
2
votes
2
replies
774
views
DESeq2: Comparison of single clinical sample to 4 normals using tumour cohort to infer dispersion of single sample
GeneExpression
DESeq2
RNASeqData
RNASeq
StatisticalMethod
15 months ago
Manthos
• 0
2
votes
3
replies
3.9k
views
TPM from STAR mapped counts
GeneExpression
GenomicFeatures
RNA-Seq
TPM
updated 17 months ago by
Gordon Smyth
52k • written 17 months ago by
gingergeiger22
• 0
0
votes
1
reply
817
views
Read methylation data from tar files on GEO (GSE73303)
GeneExpression
Bioconductor
GEOquery
MethylationArrayData
19 months ago
Arianna
• 0
2
votes
2
replies
3.1k
views
FeatureCounts Output Counts at Exon Level Using Default Settings, want gene level
GeneExpression
22 months ago
CDSPARKS
▴ 30
0
votes
1
reply
933
views
Heatmap of two conditions from the normalized gene count data
GeneExpression
heatmaps
updated 23 months ago by
ATpoint
★ 4.7k • written 24 months ago by
Amazing570
• 0
0
votes
1
reply
4.0k
views
DESeq2 after Seurat analysis on integrated datasets
DESeq2
SingleCell
GeneExpression
Seurat
updated 2.0 years ago by
ATpoint
★ 4.7k • written 2.0 years ago by
bio.boi
• 0
3
votes
4
replies
2.4k
views
Comparing different method for DESEQ2
DESeq2
GeneExpression
updated 2.4 years ago by
Michael Love
43k • written 2.4 years ago by
Vapin
• 0
0
votes
3
replies
1.4k
views
CountOverlap
GeneExpression
ChIPSeqData
updated 2.4 years ago by
James W. MacDonald
68k • written 2.4 years ago by
michela
▴ 20
0
votes
0
replies
612
views
Free Gibbson energy RNA doublex
GeneExpression
RNAinteract
2.5 years ago
Tamina
• 0
1
vote
0
replies
812
views
Error while running Case study n. 3: Integration of methylation and expression for ACC
geneexpression
methylation
2.6 years ago
hlee
▴ 10
0
votes
2
replies
1.2k
views
Seeking help in multifactor differential gene expression analysis using DESeq2. Can not get significance difference while comparing 3 time factor (…
DifferentialExpression
GeneExpression
R
enrichment
DEGseq
updated 2.6 years ago by
swbarnes2
★ 1.4k • written 2.6 years ago by
ac.mainul.mmb
• 0
0
votes
1
reply
803
views
Error "DESeqDataSetFromMatrix" function not found
GeneExpression
updated 2.6 years ago by
Basti
▴ 780 • written 2.6 years ago by
Iraide
• 0
0
votes
1
reply
879
views
Aggregate differentially expressed genes across all contrast results using DEVis
DifferentialExpression
NanoporeRNASeq
DESeq2
GeneExpression
DEVis
updated 2.7 years ago by
James W. MacDonald
68k • written 2.7 years ago by
Ana
• 0
1
vote
1
reply
1.0k
views
Error while using champ.SVD function
GeneExpression
updated 2.8 years ago by
Basti
▴ 780 • written 2.8 years ago by
Afrin
• 0
0
votes
1
reply
2.2k
views
GDCprepare of STAR - counts
GeneExpression
TCGAbiolinks
updated 2.4 years ago by
Aurora
• 0 • written 2.8 years ago by
mahsa
• 0
0
votes
0
replies
1.1k
views
Volcano plot: Putting calculated values in legend
GeneExpression
updated 2.9 years ago by
Kevin Blighe
★ 4.0k • written 2.9 years ago by
Narender Singh
• 0
2
votes
2
replies
1.1k
views
Could use some advice on analyzing ranks of expressed genes in two conditions,
GeneExpression
DESeq2
ExpressionData
updated 2.9 years ago by
fivescarynightsgames
• 0 • written 3.0 years ago by
reza
▴ 10
0
votes
2
replies
2.5k
views
Using DESeq2 for Differential Expression Analysis with Interaction Term
DifferentialExpression
GeneExpression
DESeq2
Design()
3.4 years ago
p.l.chen
• 0
2
votes
2
replies
8.0k
views
Deseq2 and log2foldchange cutoffs- How to get differentially expressed genes?
DifferentialExpression
GeneExpression
DESeq2
R
updated 3.4 years ago by
ATpoint
★ 4.7k • written 3.4 years ago by
janjoy
▴ 10
0
votes
2
replies
1.3k
views
Gene expression in R
limma
edgeR
Biobase
GeneExpression
3.5 years ago
sogol.bouyeh
• 0
2
votes
2
replies
2.1k
views
DESeq2 - one groups with no replicates
DESeq2
rnaseqGene
DifferentialExpression
GeneExpression
3.7 years ago
SUMIT
• 0
5
votes
18
replies
3.6k
views
DESeq2 comparison two cell types and two genotypes
GeneExpressionWorkflow
DESeq2
GeneExpression
updated 3.8 years ago by
Michael Love
43k • written 3.8 years ago by
Yosapol
• 0
1
vote
3
replies
1.2k
views
DESeq analysis with Biological replicate.
DESeq2
ExpressionData
GeneExpression
updated 4.1 years ago by
Michael Love
43k • written 4.1 years ago by
Javad_Sadeghi
• 0
1
vote
1
reply
891
views
Accounting for fish body mass in DE?
GeneExpression
edgeR
updated 4.1 years ago by
James W. MacDonald
68k • written 4.1 years ago by
michael.lawrence
• 0
0
votes
1
reply
920
views
Find significant genes related to a specific disease
GeneExpression
RNASeqR
limma
updated 4.2 years ago by
António Miguel de Jesus Domingues
▴ 510 • written 4.2 years ago by
Will
• 0
1
vote
3
replies
1.2k
views
Troubles implementing LIMMA for paired samples (before/after treatment) comparaisons
GeneExpression
R
limma
MultipleComparison
updated 4.2 years ago by
James W. MacDonald
68k • written 4.2 years ago by
Gwénolé
• 0
0
votes
0
replies
780
views
Diffrential Gene expression in a group of specific methylated prometor patience
GeneExpression
methyAnalysis
MethylationArray
4.3 years ago
coobzie
• 0
2
votes
5
replies
13k
views
Calculating FPKM from raw counts data using fpkm() in deseq2
deseq2
r
fpkm()
geneexpression
updated 6.4 years ago by
Michael Love
43k • written 6.4 years ago by
Beginner
▴ 60
1
vote
4
replies
2.5k
views
Need help loading gene expression data into R using either ArrayExpress or GEOquery R packages
arrayexpress
geoquery
geneexpression
updated 6.9 years ago by
Sean Davis
21k • written 6.9 years ago by
arbet003
▴ 10
3
votes
1
reply
2.1k
views
Processing several GSM from GEO database using Barcode and fRMA
frma
barcode
geneexpression
gsm
ncbi geo
updated 7.3 years ago by
Matthew McCall
▴ 830 • written 7.3 years ago by
diana.elassal
• 0
1
vote
4
replies
2.1k
views
ddCT Package for StepOnePlus qPCR output
qPCR
ddCt
geneexpression
updated 7.6 years ago by
Zhang, Jitao David
▴ 120 • written 7.6 years ago by
rbronste
▴ 60
1
vote
3
replies
1.4k
views
General suggestions regarding gene expression
geneexpression
updated 8.0 years ago by
Gordon Smyth
52k • written 8.0 years ago by
mariagiovannetti86
• 0
0
votes
0
replies
1.1k
views
Where or how to find the gene interaction with itself?
microarray
gene interaction
geneexpression
8.2 years ago
shaheryar
• 0
0
votes
0
replies
1.3k
views
Error in SAMseq analysis with R
r
samseq
microarray
rnaseq
geneexpression
8.5 years ago
namilio
• 0
0
votes
0
replies
1.4k
views
Methylation and gene expression
methylation
geneexpression
8.5 years ago
Asma rabe
▴ 290
0
votes
2
replies
1.3k
views
Methylation and gene expression data
methylation
geneexpression
updated 8.8 years ago by
arfranco
▴ 130 • written 8.8 years ago by
Asma rabe
▴ 290
0
votes
3
replies
1.8k
views
error in drawing MDS plot for eset
MDSplot
geneexpression
insilicomerging
updated 8.9 years ago by
James W. MacDonald
68k • written 8.9 years ago by
Shamim Sarhadi
▴ 20
1
vote
6
replies
2.1k
views
how to deal the effect of enormous gene expression?
deseq2
rnaseq
tomato
geneexpression
updated 9.0 years ago by
Michael Love
43k • written 9.0 years ago by
hamaor
• 0
0
votes
1
reply
1.5k
views
Error in MetaQC R package.
r
metaqc
microarray
geneexpression
9.2 years ago
Agaz Hussain Wani
▴ 260
0
votes
3
replies
2.2k
views
problem with WGCNA
geneexpression
updated 9.3 years ago by
Peter Langfelder
★ 3.0k • written 9.3 years ago by
nazaninhoseinkhan
• 0
2
votes
5
replies
2.0k
views
heatmap in R
r language
microarray
geneexpression
updated 9.5 years ago by
Axel Klenk
★ 1.1k • written 9.5 years ago by
ifaizurahredzuan
• 0
1
vote
1
reply
1.3k
views
gene expression data analysis
microarray
geneexpression
updated 9.5 years ago by
James W. MacDonald
68k • written 9.5 years ago by
ifaizurahredzuan
• 0
1
vote
1
reply
5.5k
views
NA p and p adjusted value
geneexpression
updated 9.5 years ago by
Peter Langfelder
★ 3.0k • written 9.5 years ago by
timarogger
• 0
1
vote
2
replies
6.5k
views
explanation of makeContrasts function of limma package
limma
microarray
R
geneexpression
updated 9.6 years ago by
Aaron Lun
★ 28k • written 9.6 years ago by
elisa.micarelli
▴ 10
0
votes
3
replies
12k
views
How to combine expression values of multiple probes for one gene
probe
convert
geneexpression
onechannelgui
updated 9.6 years ago by
svlachavas
▴ 840 • written 9.6 years ago by
ayanava18
▴ 10
50 results • Page
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Comment: Differential variance analysis - can i use deseq2
by
Michael Love
43k
VST is valid for working with expression data on a variance stabilized scale, so yes. There are many papers that have used VST and then com…
Answer: Use of limma R package with targeted proteomics for DE analysis
by
Gordon Smyth
52k
It is usual to analyse log normalized values from Olink in limma. Just a straight log transformation should be sufficient. We find that `tr…
Comment: WGCNA
by
pammelala304
• 0
Thank you for your time and effort. I am so grateful! Play game [block blast](https://blockblastpuzzle.io) free.
Comment: Does anyone have the command to download all of the core_nt using blast_db_get()
by
Matthew Thornton
▴ 380
Ok. So I guess the core_nt is over 300Gb compressed. I can do it, but maybe it is better to just use ncbi-tools over the internet and just…
Comment: normalizatino of dna methylation EPIC data
by
user_bioinfo
• 0
yes actually that's why I did, and its all good! thank you for your response
Votes
Comment: Different annotation packages (org.Mm.eg.db, EnsDb.Mmusculus.v79, BiomaRt) retu
Answer: normalizatino of dna methylation EPIC data
Answer: Differential expression of protein coding ENSGs (Ensembl IDs) | When to biomaRt?
Suggesting changing the "reply-to" field to the mailing list address.
Answer: UniProt.ws query for single UniRef90 ID takes several minutes
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