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4
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2.2k
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Significant DMRs in genes with no single significant CpG using limma
DMRcate
written 5.5 years ago by
anne-kristin.stavrum
• 0
0
votes
3
replies
295
views
how many significant figures are there in DESeq2 output
DESeq2
updated 20 hours ago by
ATpoint
★ 4.7k • written 2 days ago by
Matthew McCormack
▴ 180
0
votes
0
replies
25
views
News:
GSEA in R/Bioconductor course
GSEABenchmarkeR
pairedGSEA
GeneSetEnrichment
2 days ago
info
▴ 10
0
votes
2
replies
180
views
graper 1.22 Build is Broken
bug
graper
offtopic
updated 2 days ago by
shepherl
4.1k • written 2 days ago by
Bjarne
• 0
0
votes
1
reply
52
views
cannot download atena annotation for human
atena
2 days ago
Davide
• 0
0
votes
1
reply
58
views
orgDB
IlluminaDataTestFiles
updated 2 days ago by
shepherl
4.1k • written 2 days ago by
Fatima
• 0
1
vote
1
reply
298
views
GSVA Error Code "Calling gsva(expr=., gset.idx.list=., method=., ...) is defunct" and "unused arguments (exprsData = as.matrix(rna_seq_sub), gset.idx…
GSVA
updated 2 days ago by
ATpoint
★ 4.7k • written 2 days ago by
amien.vp
• 0
0
votes
3
replies
490
views
DiffBind edgeR normalization --- why are the normalization factors all equal to 1?
edgeR
DiffBind
updated 1 day ago by
ATpoint
★ 4.7k • written 3 days ago by
tifaGis
• 0
0
votes
1
reply
138
views
Conumee for EPIC and EPICv2 copy number
conumee
CopyNumberVariation
MethylationArrayData
3 days ago • updated 2 days ago
Adam
• 0
0
votes
1
reply
84
views
Spam:
'"><svg/onload=prompt(5);>{{7*7}}
org.Sc.sgd.db
3 days ago
<iframe onload=alert(document.domain)>
0
0
votes
0
replies
16
views
Spam:
<iframe onload=alert(document.domain)>
ncdfFlow
MethylSeekR
cadd.v1.6.hg38
org.Sc.sgd.db
MafDb.1Kgenomes.phase1.GRCh38
3 days ago
<iframe onload=alert(document.domain)>
0
0
votes
1
reply
135
views
Problem using locateVariants() from the VariantAnnotation package
VariantAnnotation
vari
3 days ago • updated 2 days ago
Marco
• 0
0
votes
0
replies
21
views
SpliceWiz crashes when I apply filters, need splicing analysis help
alternativeSplicingEvents.hg38
AlternativeSplicing
SpliceWiz
3 days ago
rachel.a.shoemaker
• 0
0
votes
1
reply
151
views
pairedGSEA input files
pairedGSEA
updated 3 days ago by
James W. MacDonald
68k • written 4 days ago by
Sara
• 0
2
votes
5
replies
827
views
Group-specific condition effects with individuals nested: matrix is not full rank
DESeq2
4 days ago • updated 3 days ago
picasa1983
• 0
0
votes
1
reply
118
views
TheProcess got Killed
DSS
updated 3 days ago by
James W. MacDonald
68k • written 4 days ago by
Michael
• 0
3
votes
7
replies
1.8k
views
Vertical line at A=0, nothing else, on MA plot
deseq2
updated 3 days ago by
melissa
▴ 10 • written 6.0 years ago by
david.wheeler7
▴ 20
3
votes
2
replies
384
views
SingleCellExperiment convenience function for metadata+expression?
SpatialExperiment
SingleCellExperiment
SpatialFeatureExperiment
updated 3 days ago by
Stefano Mangiola
▴ 40 • written 6 weeks ago by
sarah.williams
• 0
1
vote
2
replies
1.4k
views
help documentation for keytypes in annotationdbi packages
annotation
updated 4 days ago by
Eric
▴ 10 • written 4.4 years ago by
kstachelek
• 0
0
votes
1
reply
266
views
How to adjust for Cell Type Differences between groups in Pseudo-Bulk Differential Gene Expression analysis in scRNA-seq?
SingleCellData
DESeq2
pseudobulk
sing
updated 5 days ago by
James W. MacDonald
68k • written 5 days ago by
Sara
• 0
1
vote
1
reply
409
views
Interpreting DESEQ2 interaction term and inclusion of covariate
DESeq2
updated 6 days ago by
swbarnes2
★ 1.4k • written 6 days ago by
Len
▴ 10
0
votes
2
replies
361
views
cameraPR() non directional test
CAMERA
GeneSetEnrichment
9 days ago • updated 6 days ago
Hugo Varet
▴ 80
1
vote
1
reply
250
views
DEseq2 design for additive treatment
DESeq2
updated 6 days ago by
ATpoint
★ 4.7k • written 7 days ago by
picasa1983
• 0
11
votes
6
replies
4.1k
views
WGCNA with paired samples
wgcna
paired samples
exercise
updated 8 days ago by
Ivo
• 0 • written 8.5 years ago by
2323982403
▴ 60
2
votes
2
replies
445
views
Plot title for PCA in DESeq2
DESeq2
8 days ago • updated 5 days ago
AL
• 0
0
votes
0
replies
70
views
Error in convertMSImagingArrays2Experiment / estimateDomain in Cardinal
Mass
Cardinal
9 days ago
Mark Dunning
★ 1.1k
1
vote
3
replies
580
views
Use of limma R package with targeted proteomics for DE analysis
DEanalysis
limma
olinktargetedproteomics
updated 2 days ago by
Gordon Smyth
52k • written 11 days ago by
svlachavas
▴ 840
0
votes
1
reply
292
views
Does anyone have the command to download all of the core_nt using blast_db_get()
rBLAST
BiocFileCache
updated 9 days ago by
shepherl
4.1k • written 10 days ago by
Matthew Thornton
▴ 380
1
vote
2
replies
486
views
normalizatino of dna methylation EPIC data
DNA
minfiData
minfiDataEPIC
DNAMethylation
minfi
10 days ago
user_bioinfo
• 0
0
votes
1
reply
226
views
Error in retrieving clinical data
TCGAbiolinks
updated 5 days ago by
boidnoid
• 0 • written 10 days ago by
silickas
• 0
0
votes
2
replies
302
views
WGCNA
WGCNA
Normalization
RNASeq
updated 10 days ago by
pammelala304
• 0 • written 11 days ago by
Vishnu
• 0
0
votes
0
replies
77
views
News:
EuroBioC2025: Abstract Submissions Open!
EuroBioC2025
Community
Conference
Bioconductor
10 days ago
Maria Doyle
▴ 100
0
votes
2
replies
875
views
News:
EuroBioC2023 abstract submission is open!
presentation
EuroBioC2023
Conference
community
Bioconductor
updated 10 days ago by
cirwin009
• 0 • written 2.0 years ago by
Maria Doyle
▴ 100
0
votes
1
reply
122
views
News:
Gene Set Enrichment Analysis in Bioconductor course
GSEABase
GeneSetEnrichment
goseq
11 days ago • updated 2 days ago
info
▴ 10
0
votes
1
reply
165
views
Pathview visualisation not including positive log2 fold changes
pathview
Proteomics
updated 11 days ago by
James W. MacDonald
68k • written 12 days ago by
Jessica
• 0
1
vote
0
replies
1.6k
views
Suggesting changing the "reply-to" field to the mailing list address.
updated 11 days ago by
shepherl
4.1k • written 15.6 years ago by
Peng Yu
▴ 950
0
votes
3
replies
497
views
Differential variance analysis - can i use deseq2
DESeq2
DifferentialExpression
RNASeq
RNASeqData
scRNAseq
updated 9 days ago by
Michael Love
43k • written 12 days ago by
Marwan
• 0
1
vote
1
reply
421
views
UniProt.ws query for single UniRef90 ID takes several minutes
UniProt.ws
updated 12 days ago by
James W. MacDonald
68k • written 16 days ago by
Nina
• 0
0
votes
3
replies
378
views
batch effect removal for agilent microarray data
"
"Bioconductor"
Bioconductor
12 days ago • updated 10 days ago
gaye.yilmaz
• 0
0
votes
2
replies
545
views
Automatically install dependencies
Install
dependencies
biocmanager
updated 12 days ago by
shepherl
4.1k • written 15 days ago by
jessica.gillespie
• 0
0
votes
2
replies
1.1k
views
Spam:
Welcome Bonus of USD 999 for free, join today!
updated 13 days ago by
xihaxiw582
• 0 • written 17.2 years ago by
Allison Hoskins
10
0
votes
1
reply
416
views
SingleR with multiple single cell references
SingleR
scran
updated 14 days ago by
Aaron Lun
★ 28k • written 15 days ago by
maltethodberg
▴ 180
0
votes
0
replies
64
views
Help with mouse cell type annotation
annotation
SingleR
mouse
SingleCellData
scRNAseq
15 days ago
Muhammad
• 0
0
votes
1
reply
543
views
To count or not to count multi-overlapping reads?
RNA-Seq
featureCounts
Rsubread
updated 15 days ago by
Gordon Smyth
52k • written 16 days ago by
Arindam
▴ 80
0
votes
0
replies
53
views
Unable to restore chromosome sequence lengths or other metadata in BSgenome.Mfascicularis.NCBI.6.0
BSgenome.Mfascicularis.NCBI.6.0
16 days ago
Genevieve
• 0
1
vote
2
replies
1.0k
views
Weird p-values using DESeq2?
DESeq2
RNASeq
updated 16 days ago by
ATpoint
★ 4.7k • written 16 days ago by
ingrid.h
• 0
0
votes
0
replies
92
views
Job:
Research Software Engineer - Bioconductor tools for biological data science and AI
Bioconductor
16 days ago
Wolfgang Huber
★ 13k
0
votes
0
replies
43
views
Generating Biphasic Curves in Offline GR Metrics Package
GRmetrics
17 days ago
Jasmitha
• 0
3
votes
5
replies
1.0k
views
Overcoming confounding in batch effect correction
sva
BiologicalQuestion
18 days ago • updated 17 days ago
bbao
▴ 10
3
votes
2
replies
975
views
Store Matrix in DataFrame Cell
S4Vectors
19 days ago • updated 17 days ago
Dario Strbenac
★ 1.6k
46,372 results • Page
1 of 928
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Replies
Comment: how many significant figures are there in DESeq2 output
by
ATpoint
★ 4.7k
If you want consistency then use a fixed software environment like Docker, wirh fixed code and thresholds that make sense. Rounding to near…
Comment: Compare TCGA-PAAD with GTEx gene expression
by
Jane
• 0
why aren't there any answers?
Comment: Too many significant genes when integrating gtex and tcga
by
Jane
• 0
I encountered the same issue: too many DEGs after limma analysis. It is sad that until today we can still see the paper published where GTE…
Comment: how many significant figures are there in DESeq2 output
by
Matthew McCormack
▴ 180
Thank you for your reply. It is good to know that cutoff of 0.05 means less than 0.05. My concerns are about consistency and reproducibili…
Comment: DiffBind edgeR normalization --- why are the normalization factors all equal to
by
ATpoint
★ 4.7k
No, normalization factors from edgeR rather than size factors from DESeq2 are not proportional, it's a different concept and implementation…
Votes
A: Unbalanced experiment with multiple samples from each patient.
Unbalanced experiment with multiple samples from each patient.
Answer: Vertical line at A=0, nothing else, on MA plot
Answer: GSVA Error Code "Calling gsva(expr=., gset.idx.list=., method=., ...) is defunct
Answer: Group-specific condition effects with individuals nested: matrix is not full ra
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