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10
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DBT JRF & PhD Scholar
BiologicalQuestion
OmicsMLRepoR
1 hour ago
Manisha
• 0
0
votes
1
reply
36
views
I am having issues with bowtie2_build
genomes
bowtiew
Index
updated 1 hour ago by
James W. MacDonald
67k • written 19 hours ago by
eduardo.pastor
• 0
0
votes
1
reply
33
views
DEG analysis across multiple timepoints, treatments and tissues
DifferentialExpression
RNASeq
DESeq2
updated 2 hours ago by
Michael Love
43k • written 1 day ago by
Sim
• 0
0
votes
0
replies
30
views
My DEXSeqDataSetFromSE is returning an Error, but all of my checks to seem to indicate that this error is not present
DifferentialSplicing
RNASeqData
DEXSeq
21 hours ago
cftattersfield
• 0
1
vote
0
replies
66
views
News:
Bioconductor Course, Kenya 2025: Applications Open!
Events
Community
Training
Africa
Bioconductor
1 day ago
Maria Doyle
▴ 100
0
votes
1
reply
77
views
How to visualize these pathways using pathview
Salmo_salar
RNASeq
KEGG
pathview
updated 1 day ago by
Guido Hooiveld
★ 4.1k • written 1 day ago by
ingrid.h
• 0
2
votes
2
replies
276
views
Selection of correct coefficients or contrasts in DESeq2
DESeq2
updated 1 day ago by
swbarnes2
★ 1.4k • written 5 days ago by
Nathan
• 0
9
votes
3
replies
1.7k
views
Job:
Postdoc/RA position: Software Engineer For R and High-Performance Computing
JobPosting
CuratedAtlasQueryR
tidySpatialExperiment
tidyomics
tidySingleCellExperiment
written 7 months ago by
Stefano Mangiola
▴ 20
0
votes
4
replies
618
views
Why select top highly expressed genes based on normalized counts, but then plot VST-transformed values in DESeq2 pheatmap?
RNASeq
DESeq2
3 days ago • updated 1 day ago
Quang NN
▴ 10
1
vote
3
replies
356
views
Csaw normalization strategies - general inquiry
ChIPSeq
csaw
updated 1 day ago by
franknappi14
• 0 • written 4 days ago by
Luca
• 0
0
votes
2
replies
185
views
How to compare expression levels of genes between scRNAseq and bulkRNAseq?
Seurat
RNASeq
DESeq2
scRNAseq
3 days ago • updated 1 day ago
Quang NN
▴ 10
4
votes
9
replies
2.0k
views
Issues with seqlevelsStyle when making custom txdb objects for genomes/annotations from ToxoDB
txdbmaker
GenomeInfoDb
GenomicFeatures
Biostrings
gDNAx
updated 4 days ago by
Robert Castelo
★ 3.4k • written 6 weeks ago by
rohitsatyam102
▴ 20
0
votes
1
reply
124
views
WGCNA sample-data preprocessing order
Microarray
WGCNA
GeneExpression
updated 5 days ago by
ATpoint
★ 4.6k • written 9 days ago by
Reuben
• 0
0
votes
2
replies
124
views
WGCNA: Can we use high power (26) for signed network?
Networkanalysis
RNA-seqdata
Powerselection
WGCNA
8 days ago • updated 5 days ago
Vinesh
• 0
0
votes
1
reply
90
views
Ph.D. scholar
WGCNA
DataNormalization
MicroarrayData
updated 5 days ago by
ATpoint
★ 4.6k • written 5 days ago by
Harshul
• 0
0
votes
1
reply
115
views
Unable to find sample sheet file for ChAMP methylation data analysis
MethylationArray
MethylationArrayData
methylationArrayAnalysis
updated 5 days ago by
James W. MacDonald
67k • written 6 days ago by
Anki
• 0
0
votes
2
replies
244
views
How to generate circular cnetplot and color nodes and edges in the same plot ?
cnetplot
DESeq2
7 days ago • updated 5 days ago
Blandine
• 0
0
votes
4
replies
203
views
Is there a way to do DEG analysis across two batches sharing one sample only?
DESeq2
DifferentialExpression
RNASeqData
BatchEffect
7 days ago
georgeyu703
• 0
0
votes
1
reply
105
views
affymetrix
genomes
Annotation
updated 8 days ago by
James W. MacDonald
67k • written 8 days ago by
aurel.popa2011
• 0
0
votes
1
reply
150
views
Problems with GSEA
GSEABase
updated 8 days ago by
Guido Hooiveld
★ 4.1k • written 8 days ago by
Sidra.Gull
• 0
0
votes
1
reply
145
views
Error compiling Rbowtie2 in R-4.4 (Bioconductor 3.20 and probably 3.19)
Rbowtie
12 days ago • updated 8 days ago
ja.garcia
• 0
1
vote
1
reply
171
views
SingleCellExperiment convenience function for metadata+expression?
SpatialExperiment
SingleCellExperiment
SpatialFeatureExperiment
updated 8 days ago by
Peter Hickey
▴ 750 • written 12 days ago by
sarah.williams
• 0
0
votes
4
replies
376
views
How to sort negative log2FC or FC in RNAseq for GSEA?
DESeq2
GSEA
clusterProfiler
9 days ago • updated 7 days ago
Quang NN
▴ 10
2
votes
4
replies
351
views
EdgeR Biological CVs
edgeR
updated 5 days ago by
Gordon Smyth
52k • written 9 days ago by
James
• 0
0
votes
1
reply
229
views
Curiously shaped volcano plot with using oarfish + swish to do differential gene expression on long-read RNA-seq
fishpond
fish
updated 10 days ago by
Michael Love
43k • written 12 days ago by
brown.annaleigh
• 0
0
votes
0
replies
33
views
Problem with PCAtool as dependencie
COTAN
PCAtools
11 days ago
Silvia
• 0
1
vote
2
replies
226
views
Detecting genomic signatures of drug resistance breast cancer in transcriptomic data
TransOmicsData
signatureSearchData
StatisticalMethod
written 9 weeks ago by
olusolaxx
• 0
0
votes
2
replies
46k
views
SingleR Label.Ont
SingleR
written 16 months ago by
saeed.khalili
• 0
0
votes
0
replies
41
views
cnvEQTL : Error in cpm.default(y, lib.size = lib.size) : NA counts not allowed
CNVRanger
12 days ago
xiaofeiwang18266
▴ 50
2
votes
5
replies
717
views
Differential expression of protein coding ENSGs (Ensembl IDs) | When to biomaRt?
limma
edgeR
biomaRt
updated 12 days ago by
James W. MacDonald
67k • written 13 days ago by
Jonathan
▴ 10
0
votes
1
reply
109
views
XCMS Data Processing
r
xcms
metabolomi
NTA
Metabolomics
updated 13 days ago by
James W. MacDonald
67k • written 14 days ago by
raklein
• 0
0
votes
2
replies
224
views
Error using useMart or useEnsembl
biomaRt
updated 13 days ago by
James W. MacDonald
67k • written 13 days ago by
rayanelkholdi
• 0
2
votes
1
reply
536
views
Error in download.file for TCGAutils::symbolsToRanges
TCGAutils
updated 13 days ago by
James W. MacDonald
67k • written 13 days ago by
xiaofeiwang18266
▴ 50
0
votes
11
replies
644
views
Problems with oarfish + tximport + swish
fishpond
tximport
tximeta
updated 9 days ago by
Im
• 0 • written 14 days ago by
brown.annaleigh
• 0
0
votes
2
replies
243
views
DESeq2 Heatmap
heatmaps
updated 13 days ago by
swbarnes2
★ 1.4k • written 14 days ago by
pm_25
• 0
0
votes
4
replies
296
views
Biomart and ensembl not working?
ensembldb
biomaRt
updated 14 days ago by
James W. MacDonald
67k • written 14 days ago by
Zuzer
• 0
0
votes
1
reply
111
views
Unknown gene transcripts after annotatr
annotatr
updated 14 days ago by
James W. MacDonald
67k • written 14 days ago by
Zach
• 0
0
votes
1
reply
99
views
Improve performance of qusage aggregateGeneSet
qusage
updated 14 days ago by
James W. MacDonald
67k • written 14 days ago by
oliverd
• 0
0
votes
0
replies
89
views
gsameth GSA: probe annotation to multiple genes
missMethyl
gsameth
15 days ago
nurkenber
• 0
0
votes
0
replies
108
views
Testing Differential Expression with Interaction Effect Between Categorical and Continuous Variable with Repeated (Paired) Measures using variancePar…
limma
variancePartition
16 days ago
GJ
• 0
0
votes
1
reply
95
views
promoters_txdb <- promoters(txdb, upstream = 2000) is not 2000
TxDb.Hsapiens.UCSC.hg38.knownGene
updated 16 days ago by
James W. MacDonald
67k • written 16 days ago by
linouhao
▴ 20
0
votes
0
replies
76
views
News:
Online course: Gene set enrichment analysis in R/Bioconductor
GSEABenchmarkeR
17 days ago
info
▴ 10
0
votes
1
reply
156
views
org.Hs.eg.db issue
org.Hs.eg.db
updated 15 days ago by
shepherl
4.1k • written 17 days ago by
k.panov
• 0
0
votes
2
replies
367
views
Paired analysis in proteomics data for DE while interested in only a particular subset of the data
limma
modelmatrix
pairedanalysis
Proteomics
written 19 days ago by
svlachavas
▴ 840
0
votes
2
replies
250
views
iam not able to do retrive data using GEOquery R package
HELP
updated 19 days ago by
James W. MacDonald
67k • written 19 days ago by
ramya20kumar20
• 0
2
votes
5
replies
312
views
Differential variability modelling with vst() expression
limma
DESeq2
written 19 days ago by
jtsy6383
• 0
0
votes
2
replies
144
views
Installation error for Rsamtools.
Rsamtools
updated 19 days ago by
ATpoint
★ 4.6k • written 19 days ago by
Sheila
• 0
2
votes
8
replies
561
views
Different annotation packages (org.Mm.eg.db, EnsDb.Mmusculus.v79, BiomaRt) returning drastically different numbers of transcripts
AnnotationDbi
EnsDb.Mmusculus.v79
biomaRt
org.Mm.eg.db
updated 19 days ago by
James W. MacDonald
67k • written 20 days ago by
Nicholas
• 0
0
votes
2
replies
375
views
DESeq2 analysis with transcript counts
Transcriptomics
RNASeq
DESeq2
20 days ago • updated 18 days ago
sindhuri
• 0
0
votes
0
replies
68
views
Issue when attempting to use output from DECIPHER IDtaxa (pr2 V5) with dada2/phyloseq
DECIPHER
taxonomyassignment
phyloseq
dada2
Pr2V5
20 days ago
Maggie
• 0
46,304 results • Page
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Recent ...
Replies
Answer: I am having issues with bowtie2_build
by
James W. MacDonald
67k
Is proof.fa an actual FASTA file? Have you tried using the demo data that comes with the package? See the [vignette under Build Bowtie Inde…
Answer: DEG analysis across multiple timepoints, treatments and tissues
by
Michael Love
43k
Yes, if you think the entire time course can be modified by tissue effects then you would include another set of interaction terms. I re…
Comment: How to visualize these pathways using pathview
by
Guido Hooiveld
★ 4.1k
FYI: a similar, if not identical issue has been reported before (`pathview` has problems visualizing some pathways, but not all). You likel…
Comment: Why select top highly expressed genes based on normalized counts, but then plot
by
Quang NN
▴ 10
I see. Thank you ATpoint!
Comment: how should I apply "cpg.annotate" to TCGA methylation data in hg38 for HM450K?
by
Prakash Sah
• 0
Thanks James!
Votes
C: How does edgeR cpm function calculate log(CPM) values?
Answer: Selection of correct coefficients or contrasts in DESeq2
Answer: Selection of correct coefficients or contrasts in DESeq2
A: PDCD1 missing from TxDb.Hsapiens.UCSC.hg38.knownGene?
PDCD1 missing from TxDb.Hsapiens.UCSC.hg38.knownGene?
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