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rna seq
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9
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3.4k
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How to create manhattan plot with DESeq2
deseq2
mahattan plot
RNA Seq
updated 4.3 years ago by
bernatgel
▴ 150 • written 5.3 years ago by
adeler001
• 0
2
votes
5
replies
4.0k
views
Possible batch effect correction with RNA-Seq data when the batches do not contain both biological conditions for DE analysis
batch effect
limma
rna seq
DE
updated 4.8 years ago by
Steve Lianoglou
★ 13k • written 4.8 years ago by
svlachavas
▴ 840
0
votes
3
replies
1.0k
views
Normalization of RNA seq data
RNA seq
cpm
updated 4.9 years ago by
Kevin Blighe
★ 4.0k • written 4.9 years ago by
isabel.pieterse
• 0
4
votes
4
replies
1.2k
views
Custom gene annotations for SGSeq
sgseq
alternative splicing
rna seq
updated 6 months ago by
Sara
• 0 • written 5.2 years ago by
116100016
• 0
1
vote
4
replies
1.1k
views
Difference between Fold Changes from EdgeR glmLRTest and directly calculated fold changes.
edger
RNA Seq
updated 5.2 years ago by
James W. MacDonald
68k • written 5.2 years ago by
abf
▴ 30
0
votes
0
replies
676
views
searching for specific RNA sequences
rna seq
5.2 years ago
adeler001
• 0
0
votes
2
replies
1.1k
views
using only uniquely-mapping reads for alternative splicing using SGSeq
rna seq
SGSeq
Alternative splicing
updated 5.3 years ago by
Leonard Goldstein
▴ 260 • written 5.3 years ago by
116100016
• 0
0
votes
1
reply
695
views
51 views DESeq2 RNAseq differential expression analysis
deseq2
likelihood ratio
rna seq
wald test
updated 5.4 years ago by
Michael Love
43k • written 5.4 years ago by
adeler001
• 0
0
votes
2
replies
1.2k
views
Need help cprrecting overlappinge genes on valcano plot
volcano plot
rna seq
updated 5.5 years ago by
Michael Love
43k • written 5.5 years ago by
adeler001
• 0
1
vote
5
replies
1.6k
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
mahes.muniandy
• 0
5
votes
3
replies
1.3k
views
Steep dendrograms and few modules. WGCNA on RNA seq data
RNA seq
wgcna
updated 5.7 years ago by
Peter Langfelder
★ 3.0k • written 5.7 years ago by
agustin.gonvi
▴ 20
1
vote
2
replies
2.4k
views
adapter trimming paired vs unpaired reads
rna seq
trimmomatic
trimming
adapter trimming
paired analysis
5.8 years ago
Mozart
▴ 30
0
votes
0
replies
1.9k
views
Sub clustering from hierarchical cluster used to make heat map of gene expression data
heatmap.2
hierarchical clustering
microarray
RNA seq
DEG
7.0 years ago
lawarde.ankita1
▴ 30
13 results • Page
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Answer: SingleR with multiple single cell references
by
Aaron Lun
★ 28k
> Is it necessary to piece together the individual steps manually (e.g. aggregateReference, trainSingleR, classifySingleR, combineRecompute…
Answer: Automatically install dependencies
by
ATpoint
★ 4.7k
The BiocManager, which is sort of an extended wrapper around `install.packages`, installs all R dependencies. If you see errors then this i…
Answer: To count or not to count multi-overlapping reads?
by
Gordon Smyth
52k
> How does it handle reads that map to a gene that is located in a region that also has another gene but on the alternate strand? Overlapp…
Comment: Weird p-values using DESeq2?
by
ATpoint
★ 4.7k
This does not look like normalized counts but raw counts. Consider using `counts(dds, normalized = TRUE)` or the output of `normTransform()…
Answer: Weird p-values using DESeq2?
by
Wolfgang Huber
★ 13k
Dear Ingrid, thank you for asking. Can you provide the input count matrix (you can "pseudonymize" the biological conditions and the gene n…
Votes
A: Fraction count for multi mapping reads; how to generate input file for Deseq2 fo
Comment: Weird p-values using DESeq2?
Comment: Overcoming confounding in batch effect correction
Answer: Store Matrix in DataFrame Cell
Answer: enrichGOgradient color change
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