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RNA-seq analysis with Bioconductor Carpentries workshop, Boston, Jul 31-Aug 1
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BioCAsia Virtual Meeting, 15-18 October 2020
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2018 Toronto Bioconductor Conference registration details?
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updated 7.1 years ago by
Martin Morgan
25k • written 7.1 years ago by
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Comment: Why did PCA plots look exactly the same after adjusting for batch covariate in D
by
James W. MacDonald
67k
How you analyze your data is up to you. But do note that Subset * Conditions implies that you want to find genes that respond to stimulus d…
Comment: MBDseq analysis using qsea package
by
kf
• 0
Hi Matthias, Happy new year! I wondered if it is possible to retrieve normalized value from each samples? makeTable seems only return beta…
Comment: Why did PCA plots look exactly the same after adjusting for batch covariate in D
by
Quang
• 0
Hi, James. I forgot to mention, but yes, I did run `DESeq2::DESeq()`. So, I ran `DESeq2::DESeqDataSetFromHTSeqCount`, then `DESeq2::DESeq()…
Answer: Why did PCA plots look exactly the same after adjusting for batch covariate in D
by
ATpoint
★ 4.6k
It's an FAQ in the vignette: https://bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#why-after-vst-are-there-s…
Comment: Deseq2, enrichGO and ensembl ID
by
James W. MacDonald
67k
I am not sure I understand. You say that a unique Ensembl ID maps to multiple gene symbols. That doesn't mean you have two genes (it's just…
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A: Trajectory analysis for single cell sequencing
Answer: Annotation with org.Hs.eg.db Genome Build 38
Answer: Annotation with org.Hs.eg.db Genome Build 38
Answer: Considerations behind the choice of RNAseq Differential Expression Analysis Tool
A: goana limma- extract list of DE genes and genes in the enriched GO terms?
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