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Comment: DESeq: Gene is not flagged as differentially expressed despite count data showin
by
ATpoint
★
4.8k
Underpowerment and notable noise/variability, that's the answer.
Comment: DESeq - help with pair wise comparisons
by
ATpoint
★
4.8k
It's exactly this, no? https://bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#group-specific-condition-effect…
Comment: DESeq: Gene is not flagged as differentially expressed despite count data showin
by
ktz
•
0
Sorry for the repetitive questions! I'm actually just trying to understand it better so that I can explain to the wet lab biologists in my …
Comment: DESeq: Gene is not flagged as differentially expressed despite count data showin
by
ATpoint
★
4.8k
> Would you advice against running deseq separately for each comparison?
You're reducing power even more. Look, since you appear a bit "de…
Comment: DESeq: Gene is not flagged as differentially expressed despite count data showin
by
ktz
•
0
Thank you for the quick reply. Rep 1 is lower than rep 2 in only one group (V_CD45), is this sufficient to affect the results even if I inc…
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