Bioconductor Vignette

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10 results • Page 1 of 1

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Comment: How to compare expression levels of genes between scRNAseq and bulkRNAseq? by Quang NN ▴ 10

Thank you ATpoint for your response. In bulkRNAseq, I did have two cell types to compare. But, I want to also see if bulkRNAseq might have …

Answer: Postdoc/RA position: Software Engineer For R and High-Performance Computing by alishakhannight • 0

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Comment: Postdoc/RA position: Software Engineer For R and High-Performance Computing by alishakhannight • 0

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Answer: Why select top highly expressed genes based on normalized counts, but then plot by ATpoint ★ 4.6k

The choice of variable genes is typically part of a QC process. You select genes that in an unbiased fashion (unbiased because variance cal…

Comment: How to compare expression levels of genes between scRNAseq and bulkRNAseq? by ATpoint ★ 4.6k

My take is that RNA-seq (be it bulk or single-cell) is inherently relative. I don't think you can really "compare" the absolute expression …

Votes

A: DESeq2 interaction term

Comment: Recount3 data correspond to TCGA data

Answer: DESeq2- merging technical vs biological replicates

Answer: SingleCellExperiment convenience function for metadata+expression?

How to correct for tumor purity using DESeq2 to analyze TCGA tumor sample expression.

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