Showing : scRNAseq • reset
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317
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Differential variance analysis - can i use deseq2
DESeq2 DifferentialExpression RNASeq RNASeqData scRNAseq
updated 15 hours ago by 43k • written 3 days ago by • 0
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0
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46
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Help with mouse cell type annotation
annotation SingleR mouse SingleCellData scRNAseq
6 days ago Muhammad • 0
0
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3
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352
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How to compare expression levels of genes between scRNAseq and bulkRNAseq?
Seurat RNASeq DESeq2 scRNAseq
updated 18 days ago by • 0 • written 27 days ago by ▴ 10
2
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2
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518
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Should tSNE from biol. replicates look alike?
scRNAseq SingleCellExperiment batchelor
written 5 months ago by ▴ 10
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452
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Job: Postdoc Opportunity in Bioinformatics at Northwestern University (Research on Human Pregnancy and Autoimmunity)
Bioinformatics scRNAseq
7 months ago Damini • 0
1
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8
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1.2k
views
Advice on appropriateness of an offset in glmGamPoi/edgeR
DESeq2 glmGamPoi offset edgeR scRNAseq
updated 7 months ago by 52k • written 8 months ago by • 0
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0
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366
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scRNA-seq data trained model can be used for predictions on bulk RNA-seq data?
RNASeq scRNAseq
9 months ago Bibi • 0
0
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1
reply
644
views
Can you find the correct transcript in scRNA seq data
scRNAseq RNAseq
updated 10 months ago by ★ 4.7k • written 10 months ago by • 0
0
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2
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716
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Non-empty droplets versus good quality cells
CellRanger emptyDrops scRNAseq 10X
11 months ago rocanja ▴ 60
0
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1
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502
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How to select cells that represents more accurately a cluster in a scRNA-seq dataset?
scRNAseq
updated 12 months ago by ★ 4.7k • written 12 months ago by • 0
1
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2
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1.6k
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ScRNAseq analysis scran :: quickcluster Error
scRNAseq quickcluster scRNA scran
14 months ago ruba-mahmoud • 0
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2
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992
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scRNA analysis : DEG between low and high-expressing cells
scRNAseq
16 months ago Momo • 0
0
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1
reply
638
views
FALSE error when checking a pre made sample data
Rstudio scRNAseq DESeq2
updated 18 months ago by ★ 4.7k • written 18 months ago by • 0
2
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5
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1.4k
views
Recommendation for large.n in filterByExpr
scRNAseq edgeR SingleCellExperiment
18 months ago duez.tolga • 0
0
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1
reply
2.0k
views
DESeq2 error after installing BiocManager
scRNAseq RStudio DESeq2
updated 18 months ago by ★ 4.7k • written 18 months ago by • 0
4
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3
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1.4k
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Custom design for scRNA DGE with pseudobulking
scRNAseq scrna paireddesign edgeR pseudobulking
updated 18 months ago by 52k • written 18 months ago by • 0
0
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1
reply
694
views
If highly-expressed genes are upregulated, what happens to genes with lower expression after normalization?
scRNA scRNAseq
updated 19 months ago by ★ 4.7k • written 19 months ago by • 0
0
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1
reply
776
views
library(topGO) query
topGO scRNAseq
updated 19 months ago by ▴ 780 • written 19 months ago by • 0
0
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0
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613
views
adding noise to scRNA Seq counts
scRNAseq
19 months ago Jiyuan • 0
1
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11
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7.2k
views
Error: Bioconductor version cannot be validated; no internet connection?
scRNAseq
updated 15 months ago by • 0 • written 20 months ago by • 0
2
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3
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1.8k
views
P-value histogram of testLinearModel is not uniform
smart-seq2 scRNAseq scran
20 months ago Lluís Revilla Sancho ▴ 760
0
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0
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799
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News: course - single-cell RNAseq data analysis with R and Bioconductor
Workshop scRNAseq
21 months ago info ▴ 10
0
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1
reply
860
views
scRNAseq Download Errors
scRNAseq
updated 22 months ago by 4.1k • written 22 months ago by ★ 1.6k
2
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0
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2.0k
views
How to aggregate pseudobulks: Normalization & Log-Transformation
pseudobulk scRNAseq GSVA
22 months ago Tadeoye ▴ 20
0
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1
reply
1.2k
views
scRNAseq package and cell type annotations
ExperimentHub R ExperimentHubData scRNAseq SingleR
updated 23 months ago by ▴ 750 • written 23 months ago by ▴ 10
4
votes
5
replies
2.2k
views
Pseudo-bulk celltype comparison across conditions from multiple-sources
DESeq2 edgeR scRNAseq
updated 23 months ago by ▴ 750 • written 23 months ago by ▴ 10
0
votes
2
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1.2k
views
Could not find function "Standard Chromosomes"
scrna scRNAseq
updated 16 months ago by • 0 • written 23 months ago by • 0
0
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3
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1.7k
views
Single-Cell Data Simulation from Bulk RNA-seq
SingleCell RNASeq RNASeqData scRNAseq
updated 7 months ago by • 0 • written 2.3 years ago by • 0
1
vote
2
replies
1.6k
views
What number components should I use if I want to show 2D and 3D UMAP
scater UAMP scRNAseq
2.4 years ago Guandong Shang ▴ 40
1
vote
2
replies
1.2k
views
Combine scRNAseq data for bulk analysis using DESeq2
DESeq2 scRNAseq Fluidigm
2.4 years ago • updated 2.3 years ago evocanres • 0
0
votes
3
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1.5k
views
scRNA-seq: RS4' object has no attribute 'T'" in fastMNN R function
scanpy scRNAseq fastmnn r
2.4 years ago Madiha • 0
4
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5
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3.5k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils emptyDrops scRNA scRNAseq 10x
2.6 years ago • updated 2.5 years ago Peter Hickey ▴ 750
4
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2
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1.6k
views
how to do downstram analysis to cells which are subsetted after MNN batch-correction
batchelor fastMNN scRNAseq
2.5 years ago Guandong Shang ▴ 40
0
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4
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2.6k
views
how to make the "reconstructed" value in intergated scRNA-seq UMAP plot more readable
scater batchelor scRNAseq fastMNN
updated 2.5 years ago by ★ 28k • written 2.6 years ago by ▴ 40
0
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0
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1.3k
views
Job: Scientific Programmer | Altos Labs | San Diego
SpatialData scRNAseq JobPosting
2.6 years ago peter.kharchenko ▴ 30
0
votes
0
replies
941
views
Job: Postdoc position in integrated omics at Karolinska Institute, Stockholm
ATACSeq multiOmicsViz scRNAseq JobPosting
2.6 years ago Sebastian Lewandowski • 0
1
vote
4
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1.6k
views
Quality control of scRNA-seq data using mitochondrial ratio (drosophila)
Drosophila_melanogaster scRNAseq
2.6 years ago Chise ▴ 10
0
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0
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1.4k
views
how to calculate similarity between each cluster in scRNA and bulk RNA-seq
SingleR scRNAseq
2.6 years ago Guandong Shang ▴ 40
0
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2
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2.4k
views
calculateQCMetrics defunct, how to calculate the quality metrics by perCellQCMetrics
scRNAseq
updated 2.6 years ago by ★ 4.7k • written 2.6 years ago by • 0
0
votes
1
reply
1.5k
views
Error: DESeq(dds , useT = TRUE, minmu = 1e-06, test = "LRT", : likelihood ratio test requires a 'reduced' design, see ?DESeq
scRNAseq DESeq2
updated 2.7 years ago by ★ 4.7k • written 2.7 years ago by • 0
2
votes
8
replies
2.1k
views
RSEM - tximport; where is effective_length information used? Implications for scRNA?
RSEM tximport scRNAseq DESeq2
2.7 years ago • updated 2.6 years ago Kelen ▴ 10
0
votes
1
reply
975
views
scRNASeq reference at a transcript level
EWCE scRNAseq
updated 2.8 years ago by ▴ 780 • written 2.8 years ago by • 0
1
vote
2
replies
2.5k
views
unbalanced cell count and unbalanced read count across samples in pseudobulk differential expression analysis using scRNA-seq data
scRNAseq DifferentialExpression DESeq2
2.8 years ago Ismail • 0
1
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1
reply
2.3k
views
Can we use 'SCT normalization' to find anchors and 'LogNormalize' for the integration?
Normalization scRNAseq
updated 2.9 years ago by ▴ 750 • written 2.9 years ago by • 0
2
votes
2
replies
1.7k
views
scuttle:: librarySizeFactors returning non-finite values
normalizeCounts scran librarySizeFactors scuttle scRNAseq
3.0 years ago behr201 • 0
2
votes
2
replies
1.6k
views
How to deal with Expression set assembly problem?
deconvolution r scRNAseq bioinformatics
3.0 years ago Hasan Alanya • 0
0
votes
1
reply
1.3k
views
Aberrant number of cells
CellBarcode DropletUtils emptyDrops() Cellranger scRNAseq
updated 2.8 years ago by ▴ 20 • written 3.1 years ago by • 0
0
votes
0
replies
1.1k
views
Job: Postdoc Opening in Bioinformatics or Computational Biology
SpatialData scRNAseq SingleCell Omixer
3.1 years ago Sebastian Lewandowski • 0
0
votes
3
replies
1.9k
views
NA values for mitochondrial gene percentage
Seurat scRNAseq
3.2 years ago zahra.mesri95 • 0
0
votes
0
replies
831
views
scRNA technical replicates in separate runs
SingleCellExperiment scRNAseq
3.2 years ago Núria • 0
50 results • Page 1 of 1
Recent ...
Replies
Comment: Differential variance analysis - can i use deseq2 by Michael Love 43k
VST is valid for working with expression data on a variance stabilized scale, so yes. There are many papers that have used VST and then com…
Answer: Use of limma R package with targeted proteomics for DE analysis by Gordon Smyth 52k
It is usual to analyse log normalized values from Olink in limma. Just a straight log transformation should be sufficient. We find that `tr…
Comment: WGCNA by pammelala304 • 0
Thank you for your time and effort. I am so grateful! Play game [block blast](https://blockblastpuzzle.io) free.
Comment: Does anyone have the command to download all of the core_nt using blast_db_get() by Matthew Thornton ▴ 380
Ok. So I guess the core_nt is over 300Gb compressed. I can do it, but maybe it is better to just use ncbi-tools over the internet and just…
Comment: normalizatino of dna methylation EPIC data by user_bioinfo • 0
yes actually that's why I did, and its all good! thank you for your response
Votes
Comment: Different annotation packages (org.Mm.eg.db, EnsDb.Mmusculus.v79, BiomaRt) retu
Answer: normalizatino of dna methylation EPIC data
Answer: Differential expression of protein coding ENSGs (Ensembl IDs) | When to biomaRt?
Suggesting changing the "reply-to" field to the mailing list address.
Answer: UniProt.ws query for single UniRef90 ID takes several minutes
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