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News:
Announcing the GBCC2025 Logo Design Contest!
Conference
Community
Galaxy
GBCC2025
Bioconductor
4 months ago
Maria Doyle
▴ 100
0
votes
2
replies
1.2k
views
DESeq2 analysis on Galaxy. PCA plot shows my replicates do not cluster and large intragroup variability
DESeq2
Galaxy
pca
updated 2.0 years ago by
swbarnes2
★ 1.4k • written 2.0 years ago by
mbxdn1
• 0
0
votes
1
reply
1.1k
views
Controlling for sex variance in Galaxy DESeq2
DESeq2
RNASeq
Galaxy
updated 3.0 years ago by
Michael Love
43k • written 3.0 years ago by
István
• 0
0
votes
3
replies
1.9k
views
StringTie output as Ballgown input
ballgown
StringTie
galaxy
rnaseq
updated 2.7 years ago by
Kevin Blighe
★ 4.0k • written 4.6 years ago by
lakshmi9c
• 0
0
votes
1
reply
668
views
deseq2 for enrichments between different subcellular fractions?
deseq2
galaxy
updated 4.9 years ago by
Michael Love
43k • written 4.9 years ago by
mikelirastor
• 0
1
vote
3
replies
1.6k
views
Running DESeq2 inside local Galaxy instance
deseq2
galaxy
7.9 years ago
gkuffel
▴ 10
0
votes
2
replies
1.6k
views
Data analysis in R
bioconductor
next-generation sequencing
galaxy
updated 8.2 years ago by
Lucas Schiffer
▴ 240 • written 8.2 years ago by
efraim.culfa
• 0
0
votes
4
replies
4.5k
views
Using DESeq2 inside Galaxy
rnaseq
galaxy
deseq2
updated 9.0 years ago by
Michael Love
43k • written 9.0 years ago by
Marcelo Pereira
▴ 70
0
votes
2
replies
1.4k
views
Inverting order of groups when using DESeq2
DESeq2
Galaxy
updated 9.0 years ago by
Michael Love
43k • written 9.0 years ago by
Marcelo Pereira
▴ 70
9 results • Page
1 of 1
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Replies
Comment: how many significant figures are there in DESeq2 output
by
ATpoint
★ 4.7k
If you want consistency then use a fixed software environment like Docker, wirh fixed code and thresholds that make sense. Rounding to near…
Comment: Compare TCGA-PAAD with GTEx gene expression
by
Jane
• 0
why aren't there any answers?
Comment: Too many significant genes when integrating gtex and tcga
by
Jane
• 0
I encountered the same issue: too many DEGs after limma analysis. It is sad that until today we can still see the paper published where GTE…
Comment: how many significant figures are there in DESeq2 output
by
Matthew McCormack
▴ 180
Thank you for your reply. It is good to know that cutoff of 0.05 means less than 0.05. My concerns are about consistency and reproducibili…
Comment: DiffBind edgeR normalization --- why are the normalization factors all equal to
by
ATpoint
★ 4.7k
No, normalization factors from edgeR rather than size factors from DESeq2 are not proportional, it's a different concept and implementation…
Votes
A: Unbalanced experiment with multiple samples from each patient.
Unbalanced experiment with multiple samples from each patient.
Answer: Vertical line at A=0, nothing else, on MA plot
Answer: GSVA Error Code "Calling gsva(expr=., gset.idx.list=., method=., ...) is defunct
Answer: Group-specific condition effects with individuals nested: matrix is not full ra
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