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SingleCell
•
reset
0
votes
0
replies
157
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News:
Single-cell RNA-seq analysis with R/Bioconductor (online course)
SingleCellExperiment
SingleCell
11 weeks ago
info
▴ 10
0
votes
0
replies
310
views
Job:
Postdoctoral Research Associate in Computational Epigenomics
Phd
SingleCell
DNAMethylation
Shanghai
JobPosting
3 months ago
xiaolong
• 0
0
votes
0
replies
378
views
Job:
Postdoctoral fellowships in Spatial Omics and Single Cell Genomics at the University of Padova (IT)
SingleCell
Transcriptomics
SpatialDatasets
SpatialData
7 months ago
Silvio
• 0
0
votes
0
replies
601
views
Job:
Bioinformatics Spatial Omics and Proteomics Postdoc (Research Officer / Senior Research Officer) at WEHI (Melbourne, Australia)
Proteomics
JobPosting
SingleCell
Spatial
SpatialExperiment
10 months ago
Peter Hickey
▴ 740
3
votes
1
reply
1.0k
views
'Seurat' package
singlecell
seurat
updated 20 months ago by
ATpoint
★ 4.6k • written 20 months ago by
Sooni
▴ 10
0
votes
1
reply
987
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 20 months ago by
Peter Hickey
▴ 740 • written 20 months ago by
sandra.garces.19
• 0
0
votes
2
replies
865
views
Why my log2fcs are too high?
DESeq2
SingleCell
log2FC
20 months ago
paria.alipour
• 0
0
votes
2
replies
1.1k
views
Scaling and normalization by deconvolution of SMART-Seq 2 data
scran
Clustering
SingleCell
Normalization
21 months ago
Assa Yeroslaviz
★ 1.5k
1
vote
4
replies
1.0k
views
Single-cell differential expression analysis with multiple samples
SingleCell
22 months ago
iaradsouza1
• 0
0
votes
8
replies
1.5k
views
fail to annotate chromosome data to single cell object
error
SingleCell
annotate
updated 13 months ago by
yw24418
• 0 • written 22 months ago by
sandra.garces.19
• 0
0
votes
0
replies
893
views
Job:
Open position in the CBIO lab: single-cell proteomics
Proteomics
SingleCell
JobPosting
MassSpectrometry
22 months ago
Laurent Gatto
1.6k
0
votes
3
replies
1.8k
views
Knee and Inflection of DropletUtils
SingleCell
updated 22 months ago by
ATpoint
★ 4.6k • written 22 months ago by
ankuuu
• 0
0
votes
1
reply
3.7k
views
DESeq2 after Seurat analysis on integrated datasets
DESeq2
SingleCell
GeneExpression
Seurat
updated 22 months ago by
ATpoint
★ 4.6k • written 22 months ago by
bio.boi
• 0
1
vote
1
reply
1.0k
views
Pseudobulk running all groups together vs subsetting first
DESeq2
edgeR
SingleCell
updated 22 months ago by
Wolfgang Huber
★ 13k • written 22 months ago by
nhaus
▴ 70
0
votes
1
reply
876
views
Job:
Reopened: 'Omics data analyst for HPCBio, University of Illinois
SingleCell
Spatial
RNA-seq
22 months ago
Jenny Drnevich
★ 2.0k
0
votes
3
replies
1.1k
views
Tissue Level Differential Expression Analysis
DESeq2
DifferentialExpression
differentialex
Pseudobulk
SingleCell
updated 2.0 years ago by
Michael Love
43k • written 2.0 years ago by
skanda.hebbale
• 0
0
votes
1
reply
858
views
Job:
'Omics data analyst for HPCBio, University of Illinois
SingleCell
rnaseq
methylationArrayAnalysis
Spatial
written 2.1 years ago by
Jenny Drnevich
★ 2.0k
0
votes
3
replies
1.6k
views
Single-Cell Data Simulation from Bulk RNA-seq
SingleCell
RNASeq
RNASeqData
scRNAseq
updated 5 months ago by
Arun
• 0 • written 2.1 years ago by
irvin.ng
• 0
0
votes
1
reply
909
views
allele count data bioc object
SingleCell
SingleCellMultiModal
allele
updated 2.2 years ago by
Michael Love
43k • written 2.2 years ago by
oliver.crook
• 0
0
votes
0
replies
1.1k
views
Job:
Postdoctoral Fellow in Epigenomcis/Neurobiology (MGH/Harvard Medical School, Boston)
GenomicVariation
SingleCell
Autism
Neurogenesis
Epigenetics
2.2 years ago
sudhir.thakurela
• 0
0
votes
0
replies
700
views
Gene Id duplicated
SingleCell
SingleCellData
updated 2.2 years ago by
ATpoint
★ 4.6k • written 2.3 years ago by
Romina
• 0
0
votes
1
reply
966
views
Set up "mock" cell type as the reference?
DESeq2
SingleCell
updated 2.3 years ago by
Michael Love
43k • written 2.3 years ago by
Yiqi
▴ 10
0
votes
0
replies
637
views
weighting sample group
monocle
SingleCell
DifferentialExpression
2.3 years ago
Sogand
• 0
2
votes
2
replies
1.0k
views
How to detect LacZ tagged cells from single cell
SingleCell
SingleCellExperiment
SingleCellWorkflow
SingleCellData
2.3 years ago
k1m
▴ 20
0
votes
2
replies
1.3k
views
how to plot a fitting line on the testPseudotime
Pseudotime
testPseudotime
TSCAN
SingleCell
OSCA
2.3 years ago
Guandong Shang
▴ 40
2
votes
4
replies
1.2k
views
Summary of library size factor is all equal to one
SingleCell
SingleCellData
SingleCellWorkflow
SingleCellExperiment
2.3 years ago
Romina
• 0
0
votes
1
reply
1.5k
views
Error in .testForValidKeys(x, keys, keytype, fks) :
SingleCell
AnnotationDbi
updated 2.3 years ago by
James W. MacDonald
67k • written 2.3 years ago by
techgirl2022
• 0
0
votes
1
reply
1.6k
views
Error in running: classifySingleR from SingleR package
SingleR
SingleCell
updated 2.4 years ago by
ATpoint
★ 4.6k • written 2.4 years ago by
amit.korngut
• 0
1
vote
2
replies
1.8k
views
DESeq2 aggregated single cell data
pseudobulk
SingleCell
DESeq2
2.4 years ago
fluentin44
• 0
0
votes
0
replies
875
views
Differential expression with single cell data: any good two component (hurdle) models recently?
SingleCellWorkflow
DifferentialExpression
SingleCell
SingleCellMultiModal
MAST
2.5 years ago
Nik Tuzov
▴ 90
0
votes
0
replies
976
views
Performing DGE analysis with MAST on a variable with more than 2 levels
MAST
DEG
SingleCell
2.6 years ago
zroger499
• 0
2
votes
1
reply
931
views
() followed by [] within an argument in singlecell OSCA workflow
SingleCell
R
OSCA
updated 2.7 years ago by
jeroen.gilis
▴ 90 • written 2.7 years ago by
BioInfoBeginnerrr
• 0
2
votes
1
reply
2.1k
views
Convert AnnotatedDataFrame S4 object from GEO datasets to Seurat Object
biobase
SingleCellData
seurat
SingleCell
updated 2.9 years ago by
James W. MacDonald
67k • written 2.9 years ago by
NGS_enthusiast
• 0
0
votes
0
replies
989
views
Issues geting meaninfull results for single cell DGE using MAST
MAST
SingleCell
2.9 years ago
zroger499
• 0
0
votes
0
replies
1.1k
views
Job:
Postdoc Opening in Bioinformatics or Computational Biology
SpatialData
scRNAseq
SingleCell
Omixer
3.0 years ago
Sebastian Lewandowski
• 0
2
votes
2
replies
1.4k
views
velociraptor preparation
SingleCellExperiment
singlecell
velociraptor
scvelo
updated 3.0 years ago by
ATpoint
★ 4.6k • written 3.0 years ago by
Pietro
▴ 40
0
votes
0
replies
871
views
Job:
Research Scientists/Postdoctoral Candidates/Computational Biologists and Programmers excited about computational challenges in modeling CRISPR genome…
CRISPR
SingleCell
Genomics
3.2 years ago
lpinello
• 0
1
vote
2
replies
1.3k
views
Using limma duplicateCorrelation to account for donor/dataset effects in scRNA-Seq pseudobulk counts from multiple sources
limma
SingleCell
3.3 years ago
Andrew Pattison
• 0
0
votes
0
replies
894
views
Spike-In Cells for Normalization?
SpikeIn
SingleCell
3.4 years ago
mb1996
• 0
0
votes
0
replies
903
views
Job:
Computational postdoctoral fellow for cancer disparities research (Boston University)
DNASeq
RNASeq
SingleCell
3.4 years ago
camp
▴ 10
0
votes
0
replies
1.1k
views
Job:
Senior Bioinformatician - University of Alabama at Birmingham
JobPosting
Transcriptomics
SingleCell
RNA-seq
3.4 years ago
lianov
• 0
0
votes
1
reply
1.2k
views
Differentially expressed genes across three different conditions
MAST
SingleCell
diffGeneAnalysis
updated 3.5 years ago by
James W. MacDonald
67k • written 3.5 years ago by
sofia.puvogel
• 0
3
votes
2
replies
2.8k
views
Merging only those samples sequenced over multiple batches - scRNA-seq
SingleCell
SingleCellExperiment
scater
3.9 years ago
camerond
• 0
0
votes
0
replies
1.2k
views
Job:
Computational Biologist at Denali Therapeutics
Transcriptomics
SingleCell
JobPosting
4.0 years ago
Steve Lianoglou
★ 13k
0
votes
2
replies
1.7k
views
Removal of cell cycle effect using batchelor::regressBatches() and proper usage of corrected UMI matrix
scRNAseq
batchelor
SingleCell
SingleCellExperiment
scran
4.1 years ago • updated 4.0 years ago
jirkanov
• 0
3
votes
1
reply
2.1k
views
DESeq2 DE gene expression of scRNA-seq pseudobulk
DESeq2
SingleCell
updated 4.1 years ago by
Michael Love
43k • written 4.1 years ago by
Christian
▴ 10
0
votes
1
reply
1.0k
views
improving running time of runNMF
runNMF
scater
singlecell
SingleCellExperiment
updated 4.1 years ago by
Aaron Lun
★ 28k • written 4.1 years ago by
lirongrossmann
▴ 50
0
votes
0
replies
978
views
Creating a stable batch-corrected scRNA-Seq data with multiple datasets and multiple integrations
scater
singlecell
scran
BatchEffect
4.2 years ago
hamza_karakurt
▴ 60
2
votes
6
replies
2.6k
views
Discarding ambient RNA using estimateAmbience
emptyDrop
ambient
singlecell
estimateAmbience
updated 4.3 years ago by
Aaron Lun
★ 28k • written 4.3 years ago by
lirongrossmann
▴ 50
0
votes
6
replies
3.1k
views
Discrepancy between cell type and cluster using SingleCellExperiment and SingleR
singlecell
singlecellexperiment
umap
singleR
updated 4.3 years ago by
Aaron Lun
★ 28k • written 4.3 years ago by
lirongrossmann
▴ 80
50 results • Page
1 of 1
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Answer: DESeq error: counts matrix should be numeric, currently it has mode: character
by
ATpoint
★ 4.6k
R thinks your data are characters (e.g. "1", "2", instead of 1, 2) so given that your `counts_data` indeed only contains counts, do: `mo…
Comment: Any tools to convert .gff to .gtf format
by
ControlF5
• 0
Yes, the Appsierra team recently built a tool to convert .gff to .gtf format. [ControlF5][1] is providing the best software testing service…
Comment: EuroBioC2024 abstract submission is open!
by
Alan Downey
• 0
EuroBioC2024 has opened its abstract submission process, presenting an exciting opportunity for researchers to contribute to groundbreaking…
Comment: ChAMP.DMR error - Error in makeGenomicRatioSetFromMatrix
by
O'Brien
• 0
Consult the ChAMP.DMR documentation for more information on supported array types and expected data formats: https://bioconductor.org/packa…
Answer: combining raw counts and estimated counts
by
O'Brien
• 0
It is possible to combine raw counts from TCGA and estimated counts from GEO datasets (Kalisto pseudoalignment) while mitigating batch effe…
Votes
A: GWAS analysis with Illumina HumanOmni5-4 BeadChip - recommended software and wor
A: GWAS analysis with Illumina HumanOmni5-4 BeadChip - recommended software and wor
A: RNA-seq Normalisation - normalise all samples in experiment or only the ones use
Answer: Can DESeq2's design compensate for sequencing experimental design shortcomings?
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
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