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LIMMA
•
reset
0
votes
2
replies
109
views
List of DEGs belonging to an Agilent-single color microarray experiment using Limma package
limma
2 days ago • updated 1 day ago
giulia.gentile
• 0
4
votes
2
replies
427
views
Using voom-transformed counts for unsupervised analyses (PCA, Random Forest, Elastic Net): Best practices?
limma
limma-voom
updated 2 days ago by
Gordon Smyth
51k • written 2 days ago by
Ahdee
▴ 50
0
votes
2
replies
99
views
Limma Interaction Effect with Covariates
limma
DMRcate
2 days ago
kitzhu
• 0
2
votes
3
replies
789
views
[How to construct a model.matrix ]
limma
modelmatrix
updated 4 days ago by
Gordon Smyth
51k • written 7 days ago by
kyj2226
• 0
0
votes
4
replies
381
views
Limma- Print-tip loess normalisation-printer layout information does not match M row dimension
Normalization
limma
within-arraynormalisation
Printerlayout
8 days ago • updated 7 days ago
dseauer
• 0
0
votes
1
reply
163
views
makeContrastsDream for Interaction
limma
DifferentialExpression
variancePartition
dream
12 days ago • updated 6 days ago
abadgerw
• 0
0
votes
1
reply
270
views
Merge Replicates vs Mixed Model
limma
DifferentialExpression
variancePartition
dream
12 days ago • updated 6 days ago
abadgerw
• 0
1
vote
8
replies
963
views
Combining RNAseq and microarray data using edgeR/limma-voom
limma
voom
RNASeq
edgeR
Microarray
written 16 days ago by
Adam Marstrand
• 0
1
vote
2
replies
871
views
Consistent Decimals In Data After Antibody Array Background Correction Using Limma:nec Function
limma
MicroarrayData
Normalization
16 days ago • updated 15 days ago
Ai
• 0
3
votes
3
replies
826
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 25 days ago by
Satoshi
• 0
4
votes
3
replies
743
views
Analysis of 2*4 factorial design
limma
updated 21 days ago by
Gordon Smyth
51k • written 27 days ago by
Sudipta
• 0
2
votes
12
replies
1.4k
views
limma technical repeat correction
limma
lmer
duplicateCorrelation
technicalrepeats
updated 2 days ago by
Gordon Smyth
51k • written 27 days ago by
john
• 0
4
votes
6
replies
1.4k
views
LIMMA Differing P-Values for Treatment-Contrast Parameterization vs Group-Means Parameterization
limma
updated 29 days ago by
Gordon Smyth
51k • written 4 weeks ago by
abadgerw
• 0
4
votes
6
replies
1.0k
views
Zero intra-block correlation in RRBS analysis using voomLmFit with block
limma
duplicateCorrelation
edgeR
modelMatrixMeth
voomLmFit
4 weeks ago • updated 25 days ago
altintas.ali
• 0
2
votes
2
replies
275
views
Automatically generate contrasts from a model matrix / formula
limma
updated 5 weeks ago by
ATpoint
★ 4.5k • written 5 weeks ago by
Jonathan
▴ 10
3
votes
3
replies
278
views
How to subset voomLmFit (MArrayLM) object?
limma
edgeR
5 weeks ago
Guido Hooiveld
★ 4.1k
0
votes
3
replies
313
views
Need help interpreting heatmaps from limma
limma
written 5 weeks ago by
lhwh1208
• 0
2
votes
1
reply
1.4k
views
Removing Effect of Covariate in Only a Subset of Samples in a Model Containing All Samples Loses Full Rank if Factor but not Numerical
limma
updated 6 weeks ago by
James W. MacDonald
67k • written 6 weeks ago by
danphillips28
▴ 10
5
votes
4
replies
423
views
How to control batch effect if Group and batch are same?
limma
DESeq2
BatchEffect
updated 6 weeks ago by
ATpoint
★ 4.5k • written 7 weeks ago by
Bioinformagician
• 0
1
vote
2
replies
432
views
Poor dispersion fit in DESeq2
limma
DESeq2
RNASeq
edgeR
updated 7 weeks ago by
Michael Love
42k • written 8 weeks ago by
Ben
▴ 10
0
votes
0
replies
210
views
Assistance with Bulk RNA-seq Data Analysis Including Random Effect (`duplicateCorrelation` from `limma`)
limma
RNAseq123
7 weeks ago
tim.meese
• 0
0
votes
2
replies
331
views
EdgeR - Apparent Wrong Direction of logFC with Low Count Data
limma
edgeR
updated 7 weeks ago by
Gordon Smyth
51k • written 8 weeks ago by
blmurphy
• 0
0
votes
2
replies
422
views
Question on performing ANOVA-like analysis using limma
limma
updated 8 weeks ago by
Gordon Smyth
51k • written 8 weeks ago by
grubble
• 0
17
votes
9
replies
7.0k
views
How to use removeBatchEffect for removing effect of multiple confounding variables
limma
removebatcheffect
deseq2
updated 9 weeks ago by
James W. MacDonald
67k • written 8.5 years ago by
ompandey
▴ 10
2
votes
1
reply
328
views
Question: How to Set Up Contrasts for Case-Control Analysis in Limma While Adjusting for Covariates?
MethylationArray
EpigeneticsWorkflow
limma
Regression
updated 9 weeks ago by
James W. MacDonald
67k • written 9 weeks ago by
rae.alshammari
▴ 10
0
votes
1
reply
448
views
Unable to install/use limma
limma
Bioconductor
updated 11 weeks ago by
James W. MacDonald
67k • written 11 weeks ago by
Jennifer
• 0
3
votes
3
replies
470
views
voom does not seem to properly correct for library size
limma
updated 12 weeks ago by
Gordon Smyth
51k • written 12 weeks ago by
ATpoint
★ 4.5k
1
vote
5
replies
779
views
RNASeq normlization for samples with global shift in gene expression
qsmooth
DESeq2
limma
Normalization
updated 12 weeks ago by
Gordon Smyth
51k • written 3 months ago by
Xuan Yi
• 0
0
votes
3
replies
513
views
Differential expression analysis in time course experiments with DESeq2
DESeq2
limma
DifferentialExpression
updated 3 months ago by
Gordon Smyth
51k • written 3 months ago by
Jay
• 0
3
votes
4
replies
558
views
using limma trend workflow in CAMERA
CAMERA
limma
3 months ago • updated 12 weeks ago
Jay
• 0
3
votes
3
replies
522
views
One-sided tests with CAMERA-PR
CAMERA
limma
updated 3 months ago by
Gordon Smyth
51k • written 3 months ago by
Tyler Sagendorf
▴ 10
1
vote
6
replies
665
views
limma and Syntactically Invalid Factor Values
limma
updated 3 months ago by
Gordon Smyth
51k • written 4 months ago by
Dario Strbenac
★ 1.5k
1
vote
1
reply
513
views
DEG Analysis using Limma on DSP GeoMx Data: Handling Multiple ROIs per Core
limma
DSP
GeomxTools
4 months ago
Bioinformagician
• 0
4
votes
3
replies
527
views
Limma comparing gene expression between base line and one treatment
limma
updated 4 months ago by
James W. MacDonald
67k • written 4 months ago by
AL
• 0
0
votes
1
reply
451
views
How to rank DEG for GSEA using DEseq2 result ?
DESeq2
limma
GeneSetEnrichment
updated 4 months ago by
ATpoint
★ 4.5k • written 4 months ago by
picasa1983
• 0
2
votes
5
replies
595
views
Limma - 0 downreg and upreg values
GeoMx
limma
DifferentialExpression
updated 4 months ago by
James W. MacDonald
67k • written 4 months ago by
sop
• 0
4
votes
14
replies
1.6k
views
LIMMA 2x2 Factorial Design with Paired Samples
factorialdesign
limma
pairedsamples
updated 3 months ago by
Gordon Smyth
51k • written 4 months ago by
Michael E
• 0
3
votes
4
replies
796
views
Differences in Limma and Dreamlet random effects from confounded dataset
limma
dreamlet
4 months ago
jcollier
• 0
4
votes
4
replies
1.1k
views
Using both voom and removeBatchEffect, will they double adjust the data or is it the right way to perform this
limma
adjustment
voom
removeBatchEffect
covariates
5 months ago • updated 4 months ago
sukeshinik5
▴ 20
0
votes
1
reply
307
views
Deseq2 design matrix
DESeq2
Designmatrix
limma
updated 4 months ago by
James W. MacDonald
67k • written 5 months ago by
Neha
• 0
3
votes
3
replies
474
views
Warning message when running limma with covariates
limma
updated 5 months ago by
Gordon Smyth
51k • written 5 months ago by
Chris
▴ 20
0
votes
8
replies
1.5k
views
limma comparison for small sample size and how to get covariance matrix
covariancematrix
limma
5 months ago • updated 4 months ago
jiachengd
• 0
5
votes
4
replies
661
views
Limma with covariates
limma
5 months ago
Chris
▴ 20
3
votes
13
replies
1.3k
views
Limma model.matrix design with batch effect and different life stages proportion estimates
DeconRNASeq
limma
updated 5 months ago by
Masongeorge
• 0 • written 5 months ago by
ij283
• 0
3
votes
2
replies
622
views
Identifying DEGs using limma - NanoString GeoMx-NGS RNA Expression Data
NanoStringNCTools
limma
ExpressionData
RNASeq
edgeR
6 months ago
mohammedtoufiq91
▴ 10
1
vote
2
replies
449
views
Subsetting RNAseq data | voomaByGroup()
limma
6 months ago
Anna
• 0
1
vote
2
replies
590
views
Multi-factorial, longitudinal disease progression analysis with unbalanced patient sampling - duplicateCorrelation or blocking?
rna-seq
duplicateCorrelation
limma
voom
6 months ago
Dylan.Sheerin
• 0
2
votes
2
replies
674
views
error in limma , contrast.matrix Number of rows of contrast matrix must match number of coefficients in fit
limma
6 months ago
user_bioinfo
• 0
3
votes
10
replies
992
views
Extremely small p-values using Limma for proteomic data
Proteomics
limma
updated 6 months ago by
James W. MacDonald
67k • written 6 months ago by
Abbey
• 0
3
votes
2
replies
757
views
limma Intercept vs No-intercept models completely changing DMR results?
limma
DMRcate
MethylationArray
EPICV2
updated 5 months ago by
Gordon Smyth
51k • written 6 months ago by
Kim
• 0
50 results • Page
1 of 1
Recent ...
Replies
Answer: Downloading Metagenomic Data with Higher Taxonomic Units
by
Levi Waldron
★ 1.1k
I'm sorry I missed the first post! Yes, it is easy to agglomerate ranks in curatedMetagenomicData datasets. See the example in the vignette…
Comment: Differene between the Wald and LRT test for time-series gene expression data
by
Denise
• 0
This makes a lot of sense, thank you very much!
Comment: Downloading Metagenomic Data with Higher Taxonomic Units
by
elozanoe
• 0
Hi! I'm facing the same problem. Is there any easy way to output relative abundances of higher taxonomic units (up to kingdom), similar to…
Comment: Is it recommended to analyze different tissues together when using edgeR
by
Gordon Smyth
51k
Just plot the samples with plotMDS(). Alternatively you would use `voomLmFit()` with `sample.weights=TRUE` or `var.group=tissue`. In princ…
Comment: Handling Epic Array Data
by
Melisa Tecik
• 0
Thanks a lot for the help. You saved my day
Votes
A: correcting the batch effects in Limma and SVA
A: Using of limma moderated t-test with "corrected" expression matrix resulting fr
A: Using of limma moderated t-test with "corrected" expression matrix resulting fr
Answer: Differene between the Wald and LRT test for time-series gene expression data
Answer: Using voom-transformed counts for unsupervised analyses (PCA, Random Forest, Ela
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