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CyToF
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928
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flow cytometry differential expression through time
cytofWorkflow
cytof
diffcyt
4.1 years ago
achaillon
• 0
0
votes
0
replies
1.7k
views
Error from FlowSom to Cytofast
Cytofast
FlowSom
CyTOF
fcs files
4.4 years ago
domenico.somma
• 0
1
vote
5
replies
1.4k
views
CyTOF Differential Abundance Analysis using DESeq2
deseq2
mass cytometry
CyTOF
updated 4.4 years ago by
Michael Love
43k • written 4.4 years ago by
mikhael.manurung
▴ 280
0
votes
0
replies
800
views
Concatenating different runs from the same pool of barcoded CyTOF samples
batch effect
cytof
4.9 years ago
mikhael.manurung
▴ 280
1
vote
6
replies
2.1k
views
Re: Cydar. Can I Normalize CyTOF data with just one FCS file/batch and if so, which normalization mode is recommended?
Cydar
CyTOF
normalization
updated 5.4 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
ghogg
• 0
2
votes
4
replies
1.2k
views
Cydar's countCells tolerance parameter for arcsinh-transformed data
cydar
CyTOF
updated 6.0 years ago by
Aaron Lun
★ 28k • written 6.0 years ago by
mikhael.manurung
▴ 280
2
votes
2
replies
1.6k
views
Cytofkit - Trouble with writing files after performing the analysis
cytofkit
cytof
write.csv
output
updated 8.3 years ago by
chen_hao
▴ 30 • written 8.3 years ago by
cjackson13
• 0
1
vote
2
replies
3.0k
views
Errors when reading CyToF flowSet into flowCore
flowCore
flowcore
CyToF
Rtsne
updated 9.5 years ago by
Jiang, Mike
★ 1.3k • written 9.5 years ago by
clevy
▴ 10
8 results • Page
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Answer: SingleR with multiple single cell references
by
Aaron Lun
★ 28k
> Is it necessary to piece together the individual steps manually (e.g. aggregateReference, trainSingleR, classifySingleR, combineRecompute…
Answer: Automatically install dependencies
by
ATpoint
★ 4.7k
The BiocManager, which is sort of an extended wrapper around `install.packages`, installs all R dependencies. If you see errors then this i…
Answer: To count or not to count multi-overlapping reads?
by
Gordon Smyth
52k
> How does it handle reads that map to a gene that is located in a region that also has another gene but on the alternate strand? Overlapp…
Comment: Weird p-values using DESeq2?
by
ATpoint
★ 4.7k
This does not look like normalized counts but raw counts. Consider using `counts(dds, normalized = TRUE)` or the output of `normTransform()…
Answer: Weird p-values using DESeq2?
by
Wolfgang Huber
★ 13k
Dear Ingrid, thank you for asking. Can you provide the input count matrix (you can "pseudonymize" the biological conditions and the gene n…
Votes
A: Fraction count for multi mapping reads; how to generate input file for Deseq2 fo
Comment: Weird p-values using DESeq2?
Comment: Overcoming confounding in batch effect correction
Answer: Store Matrix in DataFrame Cell
Answer: enrichGOgradient color change
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