Hi I am running featureCounts() function from Rsubread package with my aligned dataset. I am using HPC clusters to perform the task.
my Rscript looks something like this.
fc <- featureCounts(files=fileNames,
annot.ext=file.path("/lustre/project/jfang5",
"Homo_sapiens_GRCh38",
"Homo_sapiens.GRCh38.105.gtf"),
isGTFAnnotationFile=TRUE,
GTF.attrType.extra = "gene_biotype", # classifies coding genes
isPairedEnd=TRUE,
GTF.featureType = "exon",
requireBothEndsMapped = T,
primaryOnly = T,
countChimericFragments = F)
But I am continuously getting errors as follows (I submitted the job by both interactively and with Slurm but either way is giving this error) :
Fatal error: Wrong thread calling 'RunFinalizers'
Fatal error: Wrong thread calling 'RunFinalizers'
Fatal error: Wrong thread calling 'RunFinalizers'
Fatal error: Wrong thread calling 'RunFinalizers'
Fatal error: Wrong thread calling 'RunFinalizers'
Fatal error: Wrong thread calling 'RunFinalizers'
Segmentation fault...
Can somebody help me with what is going wrong with this? Thank you in advance!!