Hi, I was trying to install MACS2 in RStudio but I keep on running into some issues. This is the code I was using:

 BiocManager::install("Herper")
    library(Herper)
    install_CondaTools(tools="macs2", env="PeakCalling_analysis", pathToMiniConda=myMiniconda, updateEnv = TRUE)

• Using Miniconda at: C:/Users/User/miniconda3 Traceback (most recent call last): File "C:\Users\User\MINICO~1\Lib\site-packages\conda\exception_handler.py", line 17, in __call__ return func(*args, **kwargs)

       ^^^^^^^^^^^^^^^^^^^^^   File "C:\Users\User\MINICO~1\Lib\site-packages\conda\cli\main.py", line 36,
  

  in main_subshell from ..base.context import context File "C:\Users\User\MINICO~1\Lib\site-packages\conda\base\context.py", line 33, in <module> from ..common._os.linux import linux_get_libc_version File "C:\Users\User\MINICO~1\Lib\site-packages\conda\common_os__init__.py", line 8, in <module> from .windows import get_free_space_on_windows as get_free_space File "C:\Users\User\MINICO~1\Lib\site-packages\conda\common_os\windows.py", line 11, in <module> from ctypes import ( File "C:\Users\User\MINICO~1\Lib\ctypes__init__.py", line 8, in <module> from _ctypes import Union, Structure, Array ImportError: DLL load failed while importing _ctypes: The specified module could not be found.

During handling of the above exception, another exception occurred:

Traceback (most recent call last): File "C:\Users\User\MINICO~1\Scripts\conda-script.py", line 12, in <module> sys.exit(main()) ^^^^^^ File "C:\Users\User\MINICO~1\Lib\site-packages\conda\cli\main.py", line 109, in main return conda_exception_handler(main, args, **kwargs) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Users\User\MINICO~1\Lib\site-packages\conda\exception_handler.py", line 389, in conda_exception_handler return_value = exception_handler(func, args, **kwargs) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Users\User\MINICO~1\Lib\site-packages\conda\exception_handler.py", line 20, in __call__ return self.handle_exception(exc_val, exc_tb) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Users\User\MINICO~1\Lib\site-packages\conda\exception_handler.py", line 52, in handle_exception from .exceptions import ( File "C:\Users\User\MINICO~1\Lib\site-packages\conda\exceptions.py", line 31, in <module> from .models.channel import Channel File "C:\Users\User\MINICO~1\Lib\site-packages\conda\models\channel.py", line 25, in <module> from ..base.context import Context, context File "C:\Users\User\MINICO~1\Lib\site-packages\conda\base\context.py", line 33, in <module> from ..common._os.linux import linux_get_libc_version File "C:\Users\User\MINICO~1\Lib\site-packages\conda\common_os__init__.py", line 8, in <module> from .windows import get_free_space_on_windows as get_free_space File "C:\Users\User\MINICO~1\Lib\site-packages\conda\common_os\windows.py", line 11, in <module> from ctypes import ( File "C:\Users\User\MINICO~1\Lib\ctypes__init__.py", line 8, in <module> from _ctypes import Union, Structure, Array ImportError: DLL load failed while importing _ctypes: The specified module could not be found. Error: one or more Python packages failed to install [error code 1]

R is also up to date: version.string R version 4.3.1 (2023-06-16 ucrt) I also tried running the same in Miniconda3 directly but I can also not install MACS2 here. Finally, I also tried downloading the zipped file but even than I cannot install ist. I would be very grateful for any help!

Cheers

macs2 is not available for Windows via conda. Your options are to use WSL2 to run a native Linux distro on your Windows or to use something like Docker. Bioinformatics on pure Windows won't get you far. WSL2 strongly recommended.