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Dick Beyer
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@dick-beyer-26
Last seen 10.1 years ago
Would anyone have experience or opinions about comparing hgu133a chips
with a starting amount of 3mg total RNA to some chips with a starting
amount of 1mg total RNA? I have a 12 chip experiment, but two of the
chips have the lower starting total RNA.
Visually, the two lower RNA chips are quite distinctive in comparing
boxplots for the raw data, but the gcrma results look quite
comparable.
This brings up a more general question of how much can the starting
total RNA for these types of arrays vary and still be compared using
gcrma?
Thanks for any ideas or suggestions,
Dick
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Richard P. Beyer, Ph.D. University of Washington
Tel.:(206) 616 7378 Env. & Occ. Health Sci. , Box 354695
Fax: (206) 685 4696 4225 Roosevelt Way NE, # 100
Seattle, WA 98105-6099