Concatenating different runs from the same pool of barcoded CyTOF samples
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@mikhaelmanurung-17423
Last seen 2.5 years ago
Netherlands

Hello, I would like to ask a question about CyTOF. I have a set of files from multiple acquisition periods of the same pool of barcoded samples (14 clinical samples and 1 reference samples). We couldn't measure everything in one go because of, well, clogging and stuff. Here is the ridgeline plot of CD3 expression distribution across acquisitions.

https://ibb.co/X2NbjWc

I do not think there's a significant variation of marker expression distribution across acquisitions. What do you think? Would it be acceptable to just concatenate all samples and then debarcode?

Thanks in advance for your advice.

batch effect cytof • 762 views
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