hi 

i would like a help please

dds=DESeqDataSetFromMatrix(count = count,colData = colData,design = ~condition)

Error in DESeqDataSet(se, design = design, ignoreRank) : 
  some values in assay are negative

The error says that your count data has only 1 column, but the colData has 16. You need to have as many columns in your count data as you have rows in your colData.