updated 11 months ago by
James W. MacDonald
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written 11 months ago by
Robert Castelo
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Comment: Csaw normalization strategies - general inquiry
by
Luca
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0
Hello Aaron,
About the IP efficiencies, yes definitely and for us, to complicate this experiment further, these batches were generated by …
Comment: How to compare expression levels of genes between scRNAseq and bulkRNAseq?
by
Quang NN
▴
10
Thank you ATpoint for your response. In bulkRNAseq, I did have two cell types to compare. But, I want to also see if bulkRNAseq might have …
Answer: Why select top highly expressed genes based on normalized counts, but then plot
by
ATpoint
★
4.6k
The choice of variable genes is typically part of a QC process. You select genes that in an unbiased fashion (unbiased because variance cal…
Comment: How to compare expression levels of genes between scRNAseq and bulkRNAseq?
by
ATpoint
★
4.6k
My take is that RNA-seq (be it bulk or single-cell) is inherently relative. I don't think you can really "compare" the absolute expression …
Answer: Csaw normalization strategies - general inquiry
by
Aaron Lun
★
28k
IIUC you're saying that 68 and 71 are more similar to each other than to all of the other replicates (more efficient IP, if I'm reading you…
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