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simplesinglecell
•
reset
4
votes
5
replies
2.6k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 4.5 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
C T
▴ 140
4
votes
6
replies
1.6k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
Angelos Armen
• 0
1
vote
1
reply
905
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
Angelos Armen
• 0
5
votes
1
reply
5.2k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 2.7 years ago by
Stefano Mangiola
▴ 40 • written 5.7 years ago by
shbrief
▴ 20
1
vote
5
replies
2.1k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 5.8 years ago by
Aaron Lun
★ 28k • written 5.8 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.4k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 5.8 years ago by
Aaron Lun
★ 28k • written 5.8 years ago by
Bogdan
▴ 670
4
votes
2
replies
2.1k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
Matt Mulé
• 0
1
vote
3
replies
2.0k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
Steve Pederson
▴ 170
8
votes
2
replies
3.4k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 6.5 years ago by
Aaron Lun
★ 28k • written 6.5 years ago by
Peter Hickey
▴ 750
0
votes
1
reply
1.1k
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 6.6 years ago by
Aaron Lun
★ 28k • written 6.6 years ago by
es.azadian
• 0
0
votes
1
reply
1.2k
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 7.1 years ago by
Aaron Lun
★ 28k • written 7.1 years ago by
Xingyu
• 0
1
vote
2
replies
2.1k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 7.2 years ago by
Aaron Lun
★ 28k • written 7.2 years ago by
Xingyu
• 0
3
votes
2
replies
3.9k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 7.9 years ago by
Aaron Lun
★ 28k • written 7.9 years ago by
sandmann.t
▴ 70
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Comment: Discrepancies in normalised count data vs unnormalised
by
AMgroup
• 0
Thank you for your reply Here are the count plots for one example CD44: normalised: ![enter image description here][1] unnormalised: !…
Comment: Discrepancies in normalised count data vs unnormalised
by
ATpoint
★ 4.8k
Please show examples using `plotCounts()`. Custom spreadsheets harbor the risk of parsing errors along the way which very often explains wh…
Comment: Cannot extract size factor from DESeq2 analysis
by
s.malik
• 0
Dear Mike, using tximeta and following code, is this fine to extract normalized counts after `sizeFactors(dds)` gives NULL? `dds <- DESeq…
Comment: Design formula for circadian time-course data for vst() function
by
Vishnu
• 0
Thank you so much for your reply.
Comment: Analyzing TnSeq data with DESeq2
by
Tom
• 0
[Doncaster Locksmith][1] delivers trusted, efficient security services tailored to your needs. Analyzing TnSeq data with DESeq2 may uncover…
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How can I correctly use phyloseq with Docker?
A: DESeq2::sizeFactors() function does not output the sizeFactor table.
Comment: Filtering after DESeq
A: Normalized counts from DESeq2 results in similar but not equal total read count?
A: DESeq2: Different Dispersions for different sample groups
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