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simplesingleCell
•
reset
4
votes
5
replies
2.3k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 4.1 years ago by
Aaron Lun
★ 28k • written 5.2 years ago by
C T
▴ 140
4
votes
6
replies
1.4k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 5.3 years ago by
Aaron Lun
★ 28k • written 5.3 years ago by
Angelos Armen
• 0
1
vote
1
reply
822
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 5.3 years ago by
Aaron Lun
★ 28k • written 5.3 years ago by
Angelos Armen
• 0
5
votes
1
reply
4.9k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 2.3 years ago by
Stefano Mangiola
▴ 20 • written 5.3 years ago by
shbrief
▴ 20
1
vote
5
replies
1.9k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 5.3 years ago by
Aaron Lun
★ 28k • written 5.3 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.1k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 5.3 years ago by
Aaron Lun
★ 28k • written 5.3 years ago by
Bogdan
▴ 670
4
votes
2
replies
1.9k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
Matt Mulé
• 0
1
vote
3
replies
1.9k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
Steve Pederson
▴ 170
8
votes
2
replies
2.8k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 6.1 years ago by
Aaron Lun
★ 28k • written 6.1 years ago by
Peter Hickey
▴ 740
0
votes
1
reply
1.0k
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 6.2 years ago by
Aaron Lun
★ 28k • written 6.2 years ago by
es.azadian
• 0
0
votes
1
reply
1.1k
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 6.7 years ago by
Aaron Lun
★ 28k • written 6.7 years ago by
Xingyu
• 0
1
vote
2
replies
2.0k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 6.8 years ago by
Aaron Lun
★ 28k • written 6.8 years ago by
Xingyu
• 0
3
votes
2
replies
3.6k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 7.4 years ago by
Aaron Lun
★ 28k • written 7.4 years ago by
sandmann.t
▴ 70
13 results • Page
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Comment: Issues with BSgenome.Mmusculus.UCSC.mm39 and generating motif matrix in Signac (
by
Paul
• 0
Unfortunately I've updated my code with the newest GRCm39 genome, and yet I am still getting the same error.... Is there anything else I ca…
Comment: WGCNA with TPM tables
by
ATpoint
★ 4.5k
WGCNA is not a Bioconductor package, but generally: If you want to do serious analysis then find the raw data. There is no guarantee what …
Answer: Issues with BSgenome.Mmusculus.UCSC.mm39 and generating motif matrix in Signac (
by
James W. MacDonald
67k
The `EnsDb.Mmusculus.v79` package is mm38, not mm39. If you want a current `EnsDb`, use `AnnotationHub` ``` > library(AnnotationHub) >…
Comment: Identical samples after deseq2 batch effect removal
by
Michael Love
42k
I'm adding my comment to the threaded section ... I don't have any suggestions here but whatever method you're using is not appropriate up…
Comment: ANOVA like approach of edgeR
by
Yunshun Chen
▴ 880
If you do it this way, the dispersion estimates would be much higher than they should (as the cell type difference is not accounted for in …
Votes
Answer: Issues with BSgenome.Mmusculus.UCSC.mm39 and generating motif matrix in Signac (
Comment: does anyone how to create rarefaction curves from a phyloseq object?
Answer: ANOVA like approach of edgeR
A: ANOVA-like test with edgeR
Answer: CleanUpRNAseq with a GTF that is missing mitochondria annotation data
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