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simplesingleCell
•
reset
4
votes
5
replies
2.3k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 4.2 years ago by
Aaron Lun
★ 28k • written 5.3 years ago by
C T
▴ 140
4
votes
6
replies
1.5k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 5.4 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
Angelos Armen
• 0
1
vote
1
reply
841
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 5.4 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
Angelos Armen
• 0
5
votes
1
reply
5.0k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 2.4 years ago by
Stefano Mangiola
▴ 20 • written 5.4 years ago by
shbrief
▴ 20
1
vote
5
replies
1.9k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 5.4 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.1k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 5.4 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
Bogdan
▴ 670
4
votes
2
replies
1.9k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 5.6 years ago by
Aaron Lun
★ 28k • written 5.6 years ago by
Matt Mulé
• 0
1
vote
3
replies
1.9k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 5.6 years ago by
Aaron Lun
★ 28k • written 5.6 years ago by
Steve Pederson
▴ 170
8
votes
2
replies
2.9k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 6.2 years ago by
Aaron Lun
★ 28k • written 6.2 years ago by
Peter Hickey
▴ 740
0
votes
1
reply
1.0k
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 6.3 years ago by
Aaron Lun
★ 28k • written 6.3 years ago by
es.azadian
• 0
0
votes
1
reply
1.1k
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 6.8 years ago by
Aaron Lun
★ 28k • written 6.8 years ago by
Xingyu
• 0
1
vote
2
replies
2.0k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 6.9 years ago by
Aaron Lun
★ 28k • written 6.9 years ago by
Xingyu
• 0
3
votes
2
replies
3.6k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 7.5 years ago by
Aaron Lun
★ 28k • written 7.5 years ago by
sandmann.t
▴ 70
13 results • Page
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Comment: Cross-validation with multiple control subgroups in limma
by
Ali Barry
▴ 40
Thanks so much for taking the time here, the insights are much appreciated. I suspected I'd have to ad hoc compare results if I did repeat …
Answer: Cross-validation with multiple control subgroups in limma
by
Gordon Smyth
52k
limma doesn't do cross-validation or resampling. Like most classical linear modeling procedures in statistics, limma uses a statistical mod…
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
by
Robert Castelo
★ 3.4k
Hi Hervé, thank you very much for your input into how to properly build and manage TxDb objects. gDNAx has two main inputs, one or more BAM…
Comment: Biostrings: Error writing long reads (>200 kbps) with writeQualityScaledXStringS
by
Aidan
▴ 60
Quick update: this is fixed in a branch awaiting PR to Biostrings (https://github.com/Bioconductor/Biostrings/pull/122). Should be in `deve…
Answer: Can DESeq2's design compensate for sequencing experimental design shortcomings?
by
Michael Love
43k
For statistical analysis plans, I recommend working with a local statistician or someone familiar with linear models in R. I have to reserv…
Votes
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
C: edgeR and lack of counts ID on CPM matrix
A: DESeq2 Following RSEM
A: importing RSEM data into DESeq2
A: Expected counts from RSEM in DESeq2
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