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simplesingleCell
•
reset
4
votes
5
replies
2.4k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 4.3 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
C T
▴ 140
4
votes
6
replies
1.5k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 5.4 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
Angelos Armen
• 0
1
vote
1
reply
852
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 5.4 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
Angelos Armen
• 0
5
votes
1
reply
5.0k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 2.5 years ago by
Stefano Mangiola
▴ 20 • written 5.5 years ago by
shbrief
▴ 20
1
vote
5
replies
2.0k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.2k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
Bogdan
▴ 670
4
votes
2
replies
1.9k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 5.6 years ago by
Aaron Lun
★ 28k • written 5.6 years ago by
Matt Mulé
• 0
1
vote
3
replies
1.9k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
Steve Pederson
▴ 170
8
votes
2
replies
3.0k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 6.3 years ago by
Aaron Lun
★ 28k • written 6.3 years ago by
Peter Hickey
▴ 750
0
votes
1
reply
1.1k
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 6.4 years ago by
Aaron Lun
★ 28k • written 6.4 years ago by
es.azadian
• 0
0
votes
1
reply
1.1k
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 6.9 years ago by
Aaron Lun
★ 28k • written 6.9 years ago by
Xingyu
• 0
1
vote
2
replies
2.0k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 6.9 years ago by
Aaron Lun
★ 28k • written 6.9 years ago by
Xingyu
• 0
3
votes
2
replies
3.7k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 7.6 years ago by
Aaron Lun
★ 28k • written 7.6 years ago by
sandmann.t
▴ 70
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Answer: Unable to find sample sheet file for ChAMP methylation data analysis
by
James W. MacDonald
67k
There is an example file in the extdata directory of the `minfiData` package that you could emulate.
Comment: Rain error: invalid annotation of time points
by
Dominik
• 0
Hi, I kept running into the same issue until I double checked their help and understood, that columns and rows have to be designed in anoth…
Answer: How to generate circular cnetplot and color nodes and edges in the same plot ?
by
Guido Hooiveld
★ 4.1k
Since the release of `enrichplot` of October 2024, the code of the function `cnetplot` has been completely rewritten by Guangchuang in orde…
Comment: CUT&Tag differential peak analysis using Diffbind, with pre-calculated normaliza
by
mec25
• 0
I am unable to find the math behind the dba.normalize(), for instance when the E-coli fraction is used is the signal mutliplied by the fact…
Comment: How to sort negative log2FC or FC in RNAseq for GSEA?
by
Quang NN
▴ 10
Thank you so much for taking time to provide this clarification!
Votes
Answer: DESeq2- merging technical vs biological replicates
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How to correct for tumor purity using DESeq2 to analyze TCGA tumor sample expression.
Answer: EdgeR Biological CVs
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