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simpleSingleCell
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reset
4
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5
replies
2.4k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 4.3 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
C T
▴ 140
4
votes
6
replies
1.5k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
Angelos Armen
• 0
1
vote
1
reply
854
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
Angelos Armen
• 0
5
votes
1
reply
5.1k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 2.5 years ago by
Stefano Mangiola
▴ 20 • written 5.5 years ago by
shbrief
▴ 20
1
vote
5
replies
2.0k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.2k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
Bogdan
▴ 670
4
votes
2
replies
1.9k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
Matt Mulé
• 0
1
vote
3
replies
1.9k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
Steve Pederson
▴ 170
8
votes
2
replies
3.0k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 6.3 years ago by
Aaron Lun
★ 28k • written 6.3 years ago by
Peter Hickey
▴ 750
0
votes
1
reply
1.1k
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 6.4 years ago by
Aaron Lun
★ 28k • written 6.4 years ago by
es.azadian
• 0
0
votes
1
reply
1.1k
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 6.9 years ago by
Aaron Lun
★ 28k • written 6.9 years ago by
Xingyu
• 0
1
vote
2
replies
2.1k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 7.0 years ago by
Aaron Lun
★ 28k • written 7.0 years ago by
Xingyu
• 0
3
votes
2
replies
3.7k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 7.6 years ago by
Aaron Lun
★ 28k • written 7.6 years ago by
sandmann.t
▴ 70
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Comment: input for singR function
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iamin053
• 0
Hi Paria, I'm wondering if you were able to get an answer on this please. I would greatly appreciate any comments!
Comment: build samplesheet and plot profile
by
yf
• 0
![enter image description here][1] [1]: /media/images/8ea93bd0-bc90-443d-a332-4701f54a
Answer: I am having issues with bowtie2_build
by
James W. MacDonald
67k
Is proof.fa an actual FASTA file? Have you tried using the demo data that comes with the package? See the [vignette under Build Bowtie Inde…
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by
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43k
Yes, if you think the entire time course can be modified by tissue effects then you would include another set of interaction terms. I re…
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by
Guido Hooiveld
★ 4.1k
FYI: a similar, if not identical issue has been reported before (`pathview` has problems visualizing some pathways, but not all). You likel…
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