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qpcr
•
reset
0
votes
0
replies
423
views
nondetects and technical replicates
qPCR
nondetects
12 months ago
Ed Siefker
▴ 230
0
votes
0
replies
777
views
How to perform a cluster analysis in RT-qPCR or HT-qPCR results?
cluster
qpcr
qpcrNorm
clusterProfiler
HTqPCR
2.1 years ago
Javier
• 0
0
votes
2
replies
1.8k
views
log2FoldChange calculation in DESeq2
DESeq2
RNASeq
qPCR
qPCRData
3.2 years ago
Claire
• 0
3
votes
0
replies
648
views
Creating qPCRset for nondetects anlaysis from QuantStudio data
nondetects
qPCR
qPCRData
3.5 years ago
jhackett
▴ 30
3
votes
4
replies
3.7k
views
Using limma for the analysis of qPCR data
NormqPCR
EasyqpcR
qPCR
HTqPCR
limma
updated 3.1 years ago by
Ming
▴ 380 • written 3.8 years ago by
enricoferrero
▴ 660
0
votes
1
reply
883
views
Most liked/utilized qPCR analysis package
qPCR
updated 6.5 years ago by
Gordon Smyth
51k • written 6.5 years ago by
rbronste
▴ 60
0
votes
0
replies
942
views
NormqPCR Package query
SelectHK
qpcr
Genorm
6.6 years ago
jingchakvk
• 0
0
votes
0
replies
968
views
HTqPCR trouble with changeCtLayout
htqpcr
qPCR
6.8 years ago
samantha_jeschonek
• 0
1
vote
7
replies
1.9k
views
EDGE: Persistent lrt() and odp() command pi0est() error due to low p values
edge
qpcr
qvalue
7.7 years ago • updated 6.8 years ago
joe.d.moore.ii
• 0
1
vote
4
replies
2.0k
views
ddCT Package for StepOnePlus qPCR output
qPCR
ddCt
geneexpression
updated 7.3 years ago by
Zhang, Jitao David
▴ 120 • written 7.3 years ago by
rbronste
▴ 60
0
votes
0
replies
985
views
Batch Correction in HTqPCR?
qPCR
htqpcr
7.8 years ago
bradley
• 0
1
vote
0
replies
2.0k
views
DE analysis of PCR array [was: dataset dim for siggenes]
qPCR
Normalization
multtest
limma
siggenes
10.2 years ago • updated 7.9 years ago
Gordon Smyth
51k
0
votes
0
replies
1.2k
views
Replicating read.qPCR - "attribute on NULL"
qpcr
R
8.2 years ago
t.f.jones
• 0
0
votes
0
replies
988
views
select samples for qPCR
qpcr
sample
9.2 years ago
Frederico Moraes Ferreira
▴ 100
1
vote
3
replies
2.7k
views
ddCt calculation using bioconductor package
ddct
qPCR
updated 3.5 years ago by
Pengcheng Yang
▴ 60 • written 9.7 years ago by
gv
▴ 40
1
vote
2
replies
1.8k
views
qRT-PCR Sample Maximization software analysis and proper experimental design advice
qPCR
Sample Maximization Analysis
Experimental Design
qRT-PCR
updated 9.6 years ago by
Gordon Smyth
51k • written 9.6 years ago by
Marcelo Laia
▴ 450
1
vote
1
reply
1.9k
views
Are the logFC confidence intervals returned by topTable adjusted for multiple comparisons?
Microarray
qPCR
limma
updated 10.2 years ago by
Gordon Smyth
51k • written 10.3 years ago by
Calin-Jageman, Robert
▴ 30
0
votes
5
replies
1.6k
views
dataset dim for siggenes
qPCR
limma
siggenes
updated 10.2 years ago by
James W. MacDonald
67k • written 10.2 years ago by
Frederico Moraes Ferreira
▴ 100
0
votes
1
reply
1.3k
views
deseq2 for pair-wise analysis
qPCR
qPCR
updated 10.3 years ago by
Michael Love
42k • written 10.3 years ago by
Guest User
★ 13k
0
votes
0
replies
1.4k
views
EasyqPCR package - extracting Standard error values
qPCR
EasyqpcR
qPCR
EasyqpcR
10.4 years ago
Tommaso Raffaello
▴ 60
1
vote
0
replies
1.1k
views
HTqPCR package data layout - how flexible is it?
qPCR
qPCR
10.5 years ago
Guest User
★ 13k
0
votes
0
replies
4.3k
views
Bioconductor 2.14 is released
BiocViews
aCGH
SNP
Sequencing
RNASeq
ChIPSeq
miRNA
Microarray
qPCR
Metabolomics
Coverage
10.6 years ago
Dan Tenenbaum
★ 8.2k
0
votes
2
replies
2.4k
views
First time use of limma for two-color agilent array analysis
qPCR
limma
GeneSpring
qPCR
limma
GeneSpring
updated 10.7 years ago by
James W. MacDonald
67k • written 10.7 years ago by
Calin-Jageman, Robert
▴ 30
0
votes
2
replies
1.7k
views
creating a qPCRset from Fluidigm 96*96 arrays in HTqPCR
qPCR
qPCR
updated 10.7 years ago by
Kipper Fletez-Brant
▴ 150 • written 10.7 years ago by
Norm Allaire
▴ 50
0
votes
5
replies
1.9k
views
SCAN.UPC for Illumina arrays?
RNASeq
qPCR
affy
oligo
frma
RNASeq
qPCR
affy
oligo
frma
updated 11.0 years ago by
Stephen Piccolo
▴ 600 • written 11.0 years ago by
Tim Triche
★ 4.2k
0
votes
0
replies
1.6k
views
ddCt calculation and statistics
qPCR
ddCt
qPCR
ddCt
11.2 years ago
Guest User
★ 13k
0
votes
0
replies
1.3k
views
test qPCR data for differential expression
qPCR
qPCR
11.4 years ago
Andreia Fonseca
▴ 810
0
votes
17
replies
3.0k
views
[Bioc] RNAseq less sensitive than microarrays? Is it a statistical issue?
Sequencing
RNASeq
Microarray
qPCR
Coverage
edgeR
DESeq
Sequencing
RNASeq
Microarray
qPCR
updated 11.5 years ago by
Steve Lianoglou
★ 13k • written 11.5 years ago by
Lucia Peixoto
▴ 330
0
votes
1
reply
4.5k
views
EdgeR vs voom, what to consider when deciding which to use
RNASeq
qPCR
edgeR
RNASeq
qPCR
edgeR
updated 11.7 years ago by
Steve Lianoglou
★ 13k • written 11.7 years ago by
Lucia Peixoto
▴ 330
0
votes
0
replies
1.2k
views
workflow from NormqPCR 5.1 to 5.3
qPCR
NormqPCR
qPCR
NormqPCR
11.7 years ago
James Perkins
▴ 120
0
votes
0
replies
1.3k
views
workflow from NormqPCR 5.1 to 5.3
qPCR
NormqPCR
qPCR
NormqPCR
11.7 years ago
Guest User
★ 13k
0
votes
2
replies
2.0k
views
reading in data in ReadqPCR
qPCR
Annotation
HTqPCR
qPCR
Annotation
HTqPCR
11.8 years ago • updated 11.7 years ago
Guest User
★ 13k
0
votes
1
reply
1.4k
views
replicating ReadqPCR
qPCR
qPCR
updated 11.7 years ago by
james perkins
▴ 300 • written 11.8 years ago by
Guest User
★ 13k
0
votes
1
reply
2.3k
views
Bioconductor Digest, Vol 120, Issue 12
Microarray
qPCR
Coverage
Annotation
Pathways
Visualization
Cancer
Breast
Organism
affy
11.8 years ago
Matthew Thornton
▴ 380
0
votes
2
replies
2.2k
views
HTqPCR problem with creation of qPCRset
qPCR
HTqPCR
qPCR
HTqPCR
updated 11.8 years ago by
James W. MacDonald
67k • written 11.8 years ago by
Elisabeth Gilis
▴ 20
0
votes
0
replies
1.2k
views
Error Bars on EdgeR
qPCR
qPCR
11.8 years ago
Ian Sudbery
▴ 30
0
votes
0
replies
1.4k
views
structure of cfx file for HTqPCR
Microarray
qPCR
cycle
HTqPCR
Microarray
qPCR
cycle
HTqPCR
11.8 years ago
Guest User
★ 13k
0
votes
0
replies
1.7k
views
Biorad iCycler cfx manager files and HTqPCR
Microarray
qPCR
HTqPCR
a4
Microarray
qPCR
HTqPCR
a4
11.8 years ago
b1gorsuch@comcast.net
▴ 20
1
vote
1
reply
1.3k
views
qRT-PCR - reading tab-delimited file (biol & tech replicates)
qPCR
ReadqPCR
qPCR
ReadqPCR
11.8 years ago
boczniak767
▴ 740
0
votes
0
replies
945
views
Using different file formats with oligo and analysis packages using oligo
SNP
Microarray
qPCR
cycle
SNP
Microarray
qPCR
cycle
11.9 years ago
Hans Thompson
▴ 30
0
votes
0
replies
1.2k
views
RankProd absurd results
qPCR
RankProd
qPCR
RankProd
12.0 years ago
Guest User
★ 13k
0
votes
0
replies
3.3k
views
Bioconductor 2.11 is released
BiocViews
SNP
Transcription
Sequencing
miRNA
Microbiome
Microarray
qPCR
Genetics
Coverage
12.1 years ago
Dan Tenenbaum
★ 8.2k
0
votes
0
replies
833
views
Analyzing expression Affymetrix Hugene1.0.st array
qPCR
qPCR
12.1 years ago
Dario Strbenac
★ 1.5k
0
votes
0
replies
998
views
sizepower
qPCR
GUI
qPCR
GUI
12.2 years ago
Michael Salbaum
▴ 80
0
votes
0
replies
1.7k
views
HTqPCR
qPCR
limma
ddCt
HTqPCR
qPCR
limma
ddCt
HTqPCR
12.2 years ago
Heidi Dvinge
★ 2.0k
0
votes
0
replies
1.2k
views
HTqPCR problems
Microarray
qPCR
GO
Preprocessing
GLAD
HTqPCR
Microarray
qPCR
GO
Preprocessing
GLAD
12.2 years ago
cfischer
▴ 10
0
votes
2
replies
2.4k
views
Fwd: help
qPCR
qPCR
updated 12.3 years ago by
Heidi Dvinge
★ 2.0k • written 12.3 years ago by
rakesh sharma
▴ 30
0
votes
0
replies
1.1k
views
HtqPCR-help
qPCR
qPCR
12.3 years ago
rakesh sharma
▴ 30
0
votes
0
replies
927
views
help
qPCR
qPCR
12.3 years ago
Guest User
★ 13k
0
votes
0
replies
954
views
help - HTqPCR and lightcycler
qPCR
HTqPCR
qPCR
HTqPCR
12.3 years ago
Heidi Dvinge
★ 2.0k
50 results • Page
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Replies
Answer: ANOVA like approach of edgeR
by
Gordon Smyth
51k
You need to clarify whether interactions are present for the two factors in your study. In the two factors do not interact, then the code y…
Answer: Create Illmina bead summary data
by
Gordon Smyth
51k
GenomeStudio produces IDAT files that you can read using the `read.idat` function in limma. That is the recommended way to read GenomeStudi…
Comment: Help with RSEM counts: do I transpose matrix?
by
NB
• 0
OK thank you.
Comment: Help with RSEM counts: do I transpose matrix?
by
James W. MacDonald
67k
You can ask here, but please read the `DESeq2` vignette first, which should answer most questions.
Comment: Help with RSEM counts: do I transpose matrix?
by
NB
• 0
Thank you. If I have questions about QC/normalization, do I ask them here or ask a new question?
Votes
Answer: ANOVA like approach of edgeR
Answer: ANOVA like approach of edgeR
Comment: ANOVA like approach of edgeR
Answer: Detecting genomic signatures of drug resistance breast cancer in transcriptom
Comment: Issues with BSgenome.Mmusculus.UCSC.mm39 and generating motif matrix in Signac (
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