Bioconductor QRTPCR

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ddCt import analysis R qRTPCR

updated 5.2 years ago by Zhang, Jitao David ▴ 120 • written 5.2 years ago by mohammedtoufiq91 ▴ 10

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Answer: Combining RNAseq and microarray data using edgeR/limma-voom by Gordon Smyth 51k

I agree with James, although I tend to combine analyses using geneset tests rather than by meta analysis tools like GeneMeta. > My questio…

Comment: Adjusted p-values become NA when sub-setting samples by txema.heredia • 0

Thanks! I've been looking into DESeq2's code and, indeed, I get matching results when running manually the code inherited from the `genefi…

Answer: DESeq2 interaction between cancer cells and microenvironment cells by Michael Love 42k

I don't really know what you mean by "come from the contamination". You can look at the main effects and the interaction, but I don't know …

Comment: incomplete official symbol mitochondrial genes in org.Hs.eg.db? by Fahd • 0

Thanks for looking into this. This issue results in the incorrect usage of multiple ORA software including EnrichR.

Comment: incomplete official symbol mitochondrial genes in org.Hs.eg.db? by Guido Hooiveld ★ 4.1k

Yes, I understand the implications of replacing the `OrgDb` packages in release. Maybe an upload to the annotationHub or GitHub could be an…

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A: p.adjust BH generates duplicate values

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