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qPCR
•
reset
0
votes
0
replies
480
views
nondetects and technical replicates
qPCR
nondetects
17 months ago
Ed Siefker
▴ 230
0
votes
0
replies
887
views
How to perform a cluster analysis in RT-qPCR or HT-qPCR results?
cluster
qpcr
qpcrNorm
clusterProfiler
HTqPCR
2.4 years ago
Javier
• 0
0
votes
2
replies
2.0k
views
log2FoldChange calculation in DESeq2
DESeq2
RNASeq
qPCR
qPCRData
3.6 years ago
Claire
• 0
3
votes
0
replies
712
views
Creating qPCRset for nondetects anlaysis from QuantStudio data
nondetects
qPCR
qPCRData
3.9 years ago
jhackett
▴ 30
3
votes
4
replies
4.2k
views
Using limma for the analysis of qPCR data
NormqPCR
EasyqpcR
qPCR
HTqPCR
limma
updated 3.4 years ago by
Ming
▴ 380 • written 4.2 years ago by
enricoferrero
▴ 680
0
votes
1
reply
960
views
Most liked/utilized qPCR analysis package
qPCR
updated 6.9 years ago by
Gordon Smyth
52k • written 6.9 years ago by
rbronste
▴ 60
0
votes
0
replies
1.0k
views
NormqPCR Package query
SelectHK
qpcr
Genorm
7.0 years ago
jingchakvk
• 0
0
votes
0
replies
1.0k
views
HTqPCR trouble with changeCtLayout
htqpcr
qPCR
7.2 years ago
samantha_jeschonek
• 0
1
vote
7
replies
2.1k
views
EDGE: Persistent lrt() and odp() command pi0est() error due to low p values
edge
qpcr
qvalue
8.1 years ago • updated 7.2 years ago
joe.d.moore.ii
• 0
1
vote
4
replies
2.1k
views
ddCT Package for StepOnePlus qPCR output
qPCR
ddCt
geneexpression
updated 7.7 years ago by
Zhang, Jitao David
▴ 120 • written 7.7 years ago by
rbronste
▴ 60
0
votes
0
replies
1.1k
views
Batch Correction in HTqPCR?
qPCR
htqpcr
8.2 years ago
bradley
• 0
1
vote
0
replies
2.1k
views
DE analysis of PCR array [was: dataset dim for siggenes]
qPCR
Normalization
multtest
limma
siggenes
10.6 years ago • updated 8.2 years ago
Gordon Smyth
52k
0
votes
0
replies
1.3k
views
Replicating read.qPCR - "attribute on NULL"
qpcr
R
8.6 years ago
t.f.jones
• 0
0
votes
0
replies
1.1k
views
select samples for qPCR
qpcr
sample
9.6 years ago
Frederico Moraes Ferreira
▴ 100
1
vote
3
replies
2.8k
views
ddCt calculation using bioconductor package
ddct
qPCR
updated 3.8 years ago by
Pengcheng Yang
▴ 60 • written 10.1 years ago by
gv
▴ 40
1
vote
2
replies
1.9k
views
qRT-PCR Sample Maximization software analysis and proper experimental design advice
qPCR
Sample Maximization Analysis
Experimental Design
qRT-PCR
updated 9.9 years ago by
Gordon Smyth
52k • written 10.0 years ago by
Marcelo Laia
▴ 450
1
vote
1
reply
2.0k
views
Are the logFC confidence intervals returned by topTable adjusted for multiple comparisons?
Microarray
qPCR
limma
updated 10.5 years ago by
Gordon Smyth
52k • written 10.7 years ago by
Calin-Jageman, Robert
▴ 30
0
votes
5
replies
1.8k
views
dataset dim for siggenes
qPCR
limma
siggenes
updated 10.6 years ago by
James W. MacDonald
68k • written 10.6 years ago by
Frederico Moraes Ferreira
▴ 100
0
votes
1
reply
1.4k
views
deseq2 for pair-wise analysis
qPCR
qPCR
updated 10.7 years ago by
Michael Love
43k • written 10.7 years ago by
Guest User
★ 13k
0
votes
0
replies
1.4k
views
EasyqPCR package - extracting Standard error values
qPCR
EasyqpcR
qPCR
EasyqpcR
10.8 years ago
Tommaso Raffaello
▴ 60
1
vote
0
replies
1.2k
views
HTqPCR package data layout - how flexible is it?
qPCR
qPCR
10.8 years ago
Guest User
★ 13k
0
votes
0
replies
4.4k
views
Bioconductor 2.14 is released
BiocViews
aCGH
SNP
Sequencing
RNASeq
ChIPSeq
miRNA
Microarray
qPCR
Metabolomics
Coverage
11.0 years ago
Dan Tenenbaum
★ 8.2k
0
votes
2
replies
2.5k
views
First time use of limma for two-color agilent array analysis
qPCR
limma
GeneSpring
qPCR
limma
GeneSpring
updated 11.1 years ago by
James W. MacDonald
68k • written 11.1 years ago by
Calin-Jageman, Robert
▴ 30
0
votes
2
replies
1.8k
views
creating a qPCRset from Fluidigm 96*96 arrays in HTqPCR
qPCR
qPCR
updated 11.1 years ago by
Kipper Fletez-Brant
▴ 150 • written 11.1 years ago by
Norm Allaire
▴ 50
0
votes
5
replies
2.1k
views
SCAN.UPC for Illumina arrays?
RNASeq
qPCR
affy
oligo
frma
RNASeq
qPCR
affy
oligo
frma
updated 11.4 years ago by
Stephen Piccolo
▴ 600 • written 11.4 years ago by
Tim Triche
★ 4.2k
0
votes
0
replies
1.7k
views
ddCt calculation and statistics
qPCR
ddCt
qPCR
ddCt
11.6 years ago
Guest User
★ 13k
0
votes
0
replies
1.4k
views
test qPCR data for differential expression
qPCR
qPCR
11.8 years ago
Andreia Fonseca
▴ 810
0
votes
17
replies
3.3k
views
[Bioc] RNAseq less sensitive than microarrays? Is it a statistical issue?
Sequencing
RNASeq
Microarray
qPCR
Coverage
edgeR
DESeq
Sequencing
RNASeq
Microarray
qPCR
updated 11.9 years ago by
Steve Lianoglou
★ 13k • written 11.9 years ago by
Lucia Peixoto
▴ 330
0
votes
1
reply
4.6k
views
EdgeR vs voom, what to consider when deciding which to use
RNASeq
qPCR
edgeR
RNASeq
qPCR
edgeR
updated 12.1 years ago by
Steve Lianoglou
★ 13k • written 12.1 years ago by
Lucia Peixoto
▴ 330
0
votes
0
replies
1.3k
views
workflow from NormqPCR 5.1 to 5.3
qPCR
NormqPCR
qPCR
NormqPCR
12.1 years ago
James Perkins
▴ 120
0
votes
0
replies
1.3k
views
workflow from NormqPCR 5.1 to 5.3
qPCR
NormqPCR
qPCR
NormqPCR
12.1 years ago
Guest User
★ 13k
0
votes
2
replies
2.1k
views
reading in data in ReadqPCR
qPCR
Annotation
HTqPCR
qPCR
Annotation
HTqPCR
12.1 years ago
Guest User
★ 13k
0
votes
1
reply
1.5k
views
replicating ReadqPCR
qPCR
qPCR
updated 12.1 years ago by
james perkins
▴ 300 • written 12.1 years ago by
Guest User
★ 13k
0
votes
1
reply
2.4k
views
Bioconductor Digest, Vol 120, Issue 12
Microarray
qPCR
Coverage
Annotation
Pathways
Visualization
Cancer
Breast
Organism
affy
12.1 years ago
Matthew Thornton
▴ 380
0
votes
2
replies
2.2k
views
HTqPCR problem with creation of qPCRset
qPCR
HTqPCR
qPCR
HTqPCR
updated 12.1 years ago by
James W. MacDonald
68k • written 12.1 years ago by
Elisabeth Gilis
▴ 20
0
votes
0
replies
1.2k
views
Error Bars on EdgeR
qPCR
qPCR
12.2 years ago
Ian Sudbery
▴ 30
0
votes
0
replies
1.5k
views
structure of cfx file for HTqPCR
Microarray
qPCR
cycle
HTqPCR
Microarray
qPCR
cycle
HTqPCR
12.2 years ago
Guest User
★ 13k
0
votes
0
replies
1.8k
views
Biorad iCycler cfx manager files and HTqPCR
Microarray
qPCR
HTqPCR
a4
Microarray
qPCR
HTqPCR
a4
12.2 years ago
b1gorsuch@comcast.net
▴ 20
1
vote
1
reply
1.4k
views
qRT-PCR - reading tab-delimited file (biol & tech replicates)
qPCR
ReadqPCR
qPCR
ReadqPCR
12.2 years ago
boczniak767
▴ 740
0
votes
0
replies
1.0k
views
Using different file formats with oligo and analysis packages using oligo
SNP
Microarray
qPCR
cycle
SNP
Microarray
qPCR
cycle
12.2 years ago
Hans Thompson
▴ 30
0
votes
0
replies
1.2k
views
RankProd absurd results
qPCR
RankProd
qPCR
RankProd
12.3 years ago
Guest User
★ 13k
0
votes
0
replies
3.4k
views
Bioconductor 2.11 is released
BiocViews
SNP
Transcription
Sequencing
miRNA
Microbiome
Microarray
qPCR
Genetics
Coverage
12.5 years ago
Dan Tenenbaum
★ 8.2k
0
votes
0
replies
889
views
Analyzing expression Affymetrix Hugene1.0.st array
qPCR
qPCR
12.5 years ago
Dario Strbenac
★ 1.6k
0
votes
0
replies
1.1k
views
sizepower
qPCR
GUI
qPCR
GUI
12.5 years ago
Michael Salbaum
▴ 80
0
votes
0
replies
1.8k
views
HTqPCR
qPCR
limma
ddCt
HTqPCR
qPCR
limma
ddCt
HTqPCR
12.6 years ago
Heidi Dvinge
★ 2.0k
0
votes
0
replies
1.3k
views
HTqPCR problems
Microarray
qPCR
GO
Preprocessing
GLAD
HTqPCR
Microarray
qPCR
GO
Preprocessing
GLAD
12.6 years ago
cfischer
▴ 10
0
votes
2
replies
2.5k
views
Fwd: help
qPCR
qPCR
updated 12.6 years ago by
Heidi Dvinge
★ 2.0k • written 12.7 years ago by
rakesh sharma
▴ 30
0
votes
0
replies
1.2k
views
HtqPCR-help
qPCR
qPCR
12.7 years ago
rakesh sharma
▴ 30
0
votes
0
replies
970
views
help
qPCR
qPCR
12.7 years ago
Guest User
★ 13k
0
votes
0
replies
1.0k
views
help - HTqPCR and lightcycler
qPCR
HTqPCR
qPCR
HTqPCR
12.7 years ago
Heidi Dvinge
★ 2.0k
50 results • Page
1 of 1
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Replies
Comment: DESeq2 - human data, weird results, troubleshooting
by
bd2000
▴ 30
I didn't include all of my code the post would be too long. Here's a link to my GitHub if anyone would like to see the full code: [Full Rst…
Answer: DESeq: Gene is not flagged as differentially expressed despite count data showin
by
James W. MacDonald
68k
The simple answer is that the within-group variability is much higher for the first gene as compared to the second. The Wald statistic is j…
Comment: DESeq: Gene is not flagged as differentially expressed despite count data showin
by
ATpoint
★ 4.8k
> since there doesn't seem to be as much count variability, No? Your CD45 samples range on normalized scale from 2 to 3255. That's 3 orde…
Comment: Ensembl site unresponsive via biomaRt
by
darylruiz6545
• 0
Thank you so much for sharing the link.
Comment: compute_fasta_digest does not match Salmon index hash
by
Alex
• 0
I use the `fastaDigest` tool from [COMBINE-lab/fastaDigest](https://github.com/COMBINE-lab/FastaDigest/), which has the function `compute_f…
Votes
Answer: DESeq: Gene is not flagged as differentially expressed despite count data showin
Two EPIC Methylation Array Analysis Questions
How to get DMR for Infinium methylation EPIC v2
A: GEOquery Fails to Download Series Matrix File
Comment: Gene IDs conversion between Ensembl and NCBI nomenclature for non-model species
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