Bioconductor Overfitting

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limma Regression overfitting

updated 3.0 years ago by Gordon Smyth 52k • written 3.0 years ago by Marek Gierlinski ▴ 30

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Comment: GSEA preranked analysis downstream of DEseq2 by Ajay • 0

`logFC * -log10(pval)` seems to be the pi-score proposed in this [paper][1] [1]: https://pmc.ncbi.nlm.nih.gov/articles/PMC3957066/

Comment: error in ChAMP loading file by janwilseo • 0

Any update on this post? I also had an issue with this. [erosion control](https://charlestonhydroseeding.com/)

Answer: CluserProfiler message "No gene can be mapped" by James W. MacDonald 68k

Two things. First, the order of your term2gene is backwards (should be term and then gene, like the name), and by default `enricher` adjust…

Comment: Controlling for batch and estrus cycle using DESeq2 in RNA-seq analysis by donnycrimson • 0

One potential workaround might be to adjust for these factors using a combination of design matrices perhaps including batch as a covariate…

Comment: DESeq2 Design controlling for gender by donnycrimson • 0

On a lighter note, while you are tackling such complex data, you might enjoy a quick break playing the [Slope Game](https://slopegame.lol)…

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