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Comment: How to sort negative log2FC or FC in RNAseq for GSEA?
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QN
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Thank you ATpoint for your response!
In the result of GSEA, like you indicated, there will be gene sets that have positive NES and gene se…
Answer: How to sort negative log2FC or FC in RNAseq for GSEA?
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ATpoint
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GSEA asks whether there are general shifts in gene expression towards certain terms. Hence, you would not split your results tables but ord…
Comment: EdgeR Biological CVs
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James
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Thank you Gordon and Joseph. I presume question (1) would be answered by using a prior G0 of 0?
For question (2), I was expecting a biolog…
Answer: EdgeR Biological CVs
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Gordon Smyth
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I think your questions are answered by the published paper. You might also like to consult recent papers on edgeR by Yunshun Chen and Pedro…
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