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ercc
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Incorporating RUVg results into ImpulseDE2 model
ImpulseDE2
ImpulseDE
ERCC
RUVSeq
3.1 years ago
Mallory
▴ 10
3
votes
2
replies
3.2k
views
Using the ERCC spike as a normalization factor for a custom algorithm
ERCC
rna-seq
edgeR
tmm normalised values
updated 7.7 years ago by
Aaron Lun
★ 28k • written 7.7 years ago by
what_theheck24
• 0
27
votes
7
replies
16k
views
Usability of ERCC spike-ins in standard RNAseq experiments
RNAseq
deseq2
ercc
updated 8.1 years ago by
Simon Anders
★ 3.8k • written 8.1 years ago by
mbeckste
▴ 30
16
votes
5
replies
7.9k
views
DESeq and Limma+Voom Normalization for Rna-Seq Data Using Ercc Spike-In
limma
voom
deseq
normalization
ercc
updated 9.0 years ago by
Gordon Smyth
51k • written 9.0 years ago by
komal.rathi
▴ 120
8
votes
5
replies
5.6k
views
edgeR and Normalization of RNAseq data using ERCC controls
edger
ercc
10.0 years ago
John
▴ 10
9
votes
4
replies
3.1k
views
News:
Experimental data package 'seqc'
seqc
rsubread
featurecounts
subjunc
ercc
News
10.0 years ago
Wei Shi
★ 3.6k
6 results • Page
1 of 1
Recent ...
Replies
Comment: ANOVA like approach of edgeR
by
Yunshun Chen
▴ 870
If you do it this way, the dispersion estimates would be much higher than they should (as the cell type difference is not accounted for in …
Comment: ANOVA like approach of edgeR
by
SamGG
▴ 350
Simply remove the cell type from the model. design <- model.matrix(~targets$Status)
Answer: ANOVA like approach of edgeR
by
Yunshun Chen
▴ 870
You could try the followings: > design <- model.matrix(~ 0 + group) > contrast <- makeContrasts(PvsL = 0.5*(L.pregnant + B.pregnan…
Answer: Identical samples after deseq2 batch effect removal
by
maripane
• 0
Thanks Michael, so is there a way to work around this issue? Can I somehow make sure I can remove this batch effects without overcorrecti…
Comment: CleanUpRNAseq with a GTF that is missing mitochondria annotation data
by
rcreed
• 0
The updated version worked well on my data. Thank you!
Votes
A: ANOVA-like test with edgeR
Answer: CleanUpRNAseq with a GTF that is missing mitochondria annotation data
Answer: CleanUpRNAseq with a GTF that is missing mitochondria annotation data
A: TopGO: How to retrieve the gene list related to a GO ID ?
A: Error in DESeqDataSet : some values in assay are not integers
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