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18
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Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
limma
DESeq2
edgeR
5 hours ago
anna.cot.anna.cot
▴ 30
6
votes
10
replies
2.4k
views
What type of counts data to import for performing Isoform analysis in edgeR
limma
isoform
edgeR
salmon
R
updated 8 days ago by
ATpoint
★ 4.6k • written 20 days ago by
mohammedtoufiq91
▴ 10
7
votes
6
replies
835
views
ANOVA like approach of edgeR
edgeR
updated 20 days ago by
Gordon Smyth
52k • written 25 days ago by
Talip
▴ 10
3
votes
3
replies
528
views
Is it recommended to analyze different tissues together when using edgeR
DifferentialExpression
edgeR
updated 27 days ago by
Gordon Smyth
52k • written 27 days ago by
Lars Roed
• 0
2
votes
4
replies
1.6k
views
Differential Expression Analysis with unbalanced batches without replication between conditions
edgeR
BatchEffect
updated 5 weeks ago by
Gordon Smyth
52k • written 5 weeks ago by
Thili
• 0
1
vote
8
replies
1.1k
views
Combining RNAseq and microarray data using edgeR/limma-voom
limma
voom
RNASeq
edgeR
Microarray
written 6 weeks ago by
Adam Marstrand
• 0
0
votes
1
reply
455
views
single cell RNA seq pseudobulk batch correction
edgeR
updated 6 weeks ago by
James W. MacDonald
67k • written 6 weeks ago by
mairahtkhan
• 0
3
votes
3
replies
887
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 7 weeks ago by
Satoshi
• 0
0
votes
4
replies
612
views
RNA-seq: samples form different experiments, how to do DGEs?
DGEs
edgeR
RNASeqData
7 weeks ago
Philippine
• 0
4
votes
6
replies
1.1k
views
Zero intra-block correlation in RRBS analysis using voomLmFit with block
limma
duplicateCorrelation
edgeR
modelMatrixMeth
voomLmFit
8 weeks ago • updated 7 weeks ago
altintas.ali
• 0
1
vote
2
replies
311
views
edgeR 4.2.2 not compiled for Windows
edger
edgeR
8 weeks ago
Hongjian
• 0
3
votes
3
replies
304
views
How to subset voomLmFit (MArrayLM) object?
limma
edgeR
9 weeks ago
Guido Hooiveld
★ 4.1k
4
votes
1
reply
334
views
edgeR 4.2.1 compression bug: a minimally reproducible snippet
edger
edgeR
updated 9 weeks ago by
Gordon Smyth
52k • written 10 weeks ago by
Hongjian
• 0
1
vote
1
reply
252
views
Normalization RNA-seq for multiple plates
Normalization
DESeq2
RNASeqData
edgeR
updated 11 weeks ago by
ATpoint
★ 4.6k • written 11 weeks ago by
Pimpa
• 0
1
vote
2
replies
472
views
Poor dispersion fit in DESeq2
limma
DESeq2
RNASeq
edgeR
updated 11 weeks ago by
Michael Love
43k • written 12 weeks ago by
Ben
▴ 10
0
votes
2
replies
368
views
EdgeR - Apparent Wrong Direction of logFC with Low Count Data
limma
edgeR
updated 11 weeks ago by
Gordon Smyth
52k • written 12 weeks ago by
blmurphy
• 0
1
vote
2
replies
332
views
Inconsistent result in edgeR
edgeR
3 months ago • updated 12 weeks ago
hiroko.ohmiya
• 0
1
vote
1
reply
457
views
Help with design matrix for RNA-seq data using edgeR
edgeR
RNASeqData
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
erik.burchard
• 0
2
votes
9
replies
839
views
Seeking Advice on Using edgeR and variancePartition for RNA-seq Data from Multiple Tissues and Patients
edgeR
variancePartition
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
Seymoo
• 0
0
votes
3
replies
521
views
comparing common differentially expressed genes from two Rna-Seq Dataset.
DESeq2
edgeR
DifferentialExpression
written 4 months ago by
dancingcow
• 0
1
vote
1
reply
711
views
Expected memory usage for analyzing large single-cell seq datasets (> 450,000 cells)?
BigData
Clustering
memory
SingleCellExperiment
edgeR
updated 4 months ago by
Aaron Lun
★ 28k • written 4 months ago by
Joseph
• 0
0
votes
1
reply
391
views
edgeR - design a complex matrix
design
model.matrix
edgeR
complexdesign
updated 4 months ago by
Gordon Smyth
52k • written 4 months ago by
Karla
• 0
3
votes
3
replies
583
views
Optimizing CPM Calculation for Varied RNA-seq Data
edgeR
updated 4 months ago by
James W. MacDonald
67k • written 4 months ago by
Ahdee
▴ 50
0
votes
4
replies
590
views
DE analysis in EdgeR /Can any one please help me.
RNASeqData
DEGraph
RNASeq
edgeR
DegNorm
written 5 months ago by
Shaozhen
• 0
1
vote
8
replies
985
views
Advice on appropriateness of an offset in glmGamPoi/edgeR
DESeq2
glmGamPoi
offset
edgeR
scRNAseq
updated 5 months ago by
Gordon Smyth
52k • written 5 months ago by
Alec
• 0
3
votes
5
replies
1.7k
views
Right way to pseudobulk donors across replicates in edgeR
pseudobulk
edgeR
DifferentialExpression
updated 5 months ago by
Reza Ghamsari
• 0 • written 9 months ago by
Jack S.
▴ 50
0
votes
4
replies
619
views
Single Cell Experiment : Differential Expression "List of length 0" - Help!
DifferentialExpression
scran
scater
SingleCellExperiment
edgeR
6 months ago
vohara4
• 0
3
votes
6
replies
839
views
Comapre two groups in edgeR- Coefficient and logFC explanation
edgeR
6 months ago
JG13
• 0
3
votes
2
replies
677
views
Identifying DEGs using limma - NanoString GeoMx-NGS RNA Expression Data
NanoStringNCTools
limma
ExpressionData
RNASeq
edgeR
7 months ago
mohammedtoufiq91
▴ 10
3
votes
3
replies
727
views
How to correctly adjust for gender in edgeR
design
glm
edgeR
updated 7 months ago by
Gordon Smyth
52k • written 7 months ago by
Guillermo
• 0
1
vote
3
replies
654
views
How to approach time course RNAseq data? Seeing 80% DE genes in timepoint groups
DifferentialExpression
TimeCourse
timeOmics
edgeR
RNAseq
updated 7 months ago by
Gordon Smyth
52k • written 7 months ago by
Ashley
• 0
0
votes
1
reply
392
views
NEED FOR NESSASORY HELP
RNASeqData
edgeR
updated 7 months ago by
Gordon Smyth
52k • written 7 months ago by
z_bahari
• 0
4
votes
2
replies
1.1k
views
Why does GSEA on edgeR results for randomized samples give highly significant p-values?
fgsea
edgeR
7 months ago
gene_bioconductor
▴ 10
1
vote
2
replies
783
views
Citation for edgeR user guide
edgeR
written 7 months ago by
k___
▴ 30
4
votes
6
replies
1.0k
views
FilterByExpr low counts with small sample size
edgeR
8 months ago • updated 7 months ago
Jonathan
▴ 10
0
votes
2
replies
397
views
How to make DGEList and design matrix by edgeR package
DGEList
edgeR
8 months ago
prity6459
• 0
0
votes
3
replies
583
views
Finding DEGs by using edgeR
subsettingdata
edgeR
designmatrix
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
prity6459
• 0
5
votes
3
replies
918
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 7 months ago by
Gordon Smyth
52k • written 8 months ago by
Shaimaa Gamal
• 0
3
votes
3
replies
921
views
permutation test in edgeR
rna-seq
edgeR
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
Netanel
• 0
1
vote
3
replies
648
views
Why is my gene not significantly differentially expressed?
limmaGUI
edgeR
limma
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
Simran
• 0
5
votes
6
replies
880
views
Not fully paired differential gene expression analysis
edgeR
DifferentialExpression
updated 8 months ago by
ATpoint
★ 4.6k • written 8 months ago by
nhaus
▴ 70
1
vote
3
replies
555
views
Lib sizes in the DGE object are different from original lib sizes
edgeR
DifferentialExpression
updated 8 months ago by
James W. MacDonald
67k • written 8 months ago by
Shaimaa Gamal
• 0
0
votes
2
replies
544
views
edgeR analysis: glm functions and offset matrix
tximport
edgeR
Normalization
8 months ago
Marianna
▴ 20
0
votes
4
replies
879
views
Adjust RNA-seq counts (more than one batch variable) for plotting differentially expressed genes
ComBat
sva
edgeR
8 months ago
Lucy
▴ 60
3
votes
5
replies
1.3k
views
Differing results from plotMDS and PCAtools
edgeR
PCAtools
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
Fabian
• 0
0
votes
6
replies
743
views
Doing comparison of RNA-seq data using EdgeR
metaMSdata
edgeR
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
Faisal
• 0
0
votes
1
reply
613
views
Using splicing junction counts with edgeR/limma-voom
splicing
limma
edgeR
updated 9 months ago by
Gordon Smyth
52k • written 9 months ago by
scoops_streams_06
• 0
6
votes
5
replies
1.8k
views
What benchmark should I use for setting the EdgeR filterByExpr min.count parameter?
edgeR
DifferentialExpression
updated 6 weeks ago by
Gordon Smyth
52k • written 9 months ago by
Jack S.
▴ 50
2
votes
3
replies
611
views
edgeR, 3 x 2 design with a batch effect and contrasts
design
edgeR
contrasts
updated 9 months ago by
Gordon Smyth
52k • written 9 months ago by
JKim
• 0
0
votes
2
replies
578
views
RNAseq analysis
rnaseqGene
edgeR
DEGraph
DifferentialExpression
updated 9 months ago by
Gordon Smyth
52k • written 9 months ago by
z_bahari
• 0
50 results • Page
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Comment: Feature Counts - potential bug with outfile.jcounts
by
chris2.a.white
• 0
Hi @gordonsmyth and @weishi, Firstly, thank you for offering your time to develop the junction quantification module further. This is gre…
Comment: how should I apply "cpg.annotate" to TCGA methylation data in hg38 for HM450K?
by
xiaofeiwang198266
• 0
Hi Tim, Thanks for your reply! Yes, my data is 450K, and it is TCGA methylation data downloaded by TCGAbiolinks. The problem is that I can …
Answer: Error in get_anno_data(ontology) : ontology not supported yet...
by
Guido Hooiveld
★ 4.1k
Did you check the help page for the function `enrichDO`? Type `?enrichDO`. I am asking, because there it is stated that the argument `ont`…
Comment: Build error on kunpeng2 Linux aarch64
by
martin.grigorov
▴ 10
Hi, There was a problem with `icu` and because of that the (r)curl library was not installed. The system/OS curl library is one version ol…
Answer: Standardization of rlog transformed counts before PCA
by
ATpoint
★ 4.6k
I guess you mean scaling in terms of Z-scoring? There are plenty of posts towards scaling in PCA both here on Bioconductor and in other com…
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Comment: Feature Counts - potential bug with outfile.jcounts
Answer: Feature Counts - potential bug with outfile.jcounts
Comment: Can we get beta values with DMRcate package
Answer: Can we get beta values with DMRcate package
DiffBind giving different results from dba.report() and dba.analyze(..., bRetrieveAnalysis=TRUE)
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