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desEQ2
•
reset
0
votes
0
replies
27
views
[ChIP-seq] DESeq2 on counts matrix from union set of called peaks
csaw
ChIPseq
DESeq2
DiffBind
10 hours ago
Luca
• 0
0
votes
1
reply
150
views
Removing UTR from GTF for gene quantification
edgeR
DESeq2
updated 1 day ago by
ATpoint
★ 4.7k • written 2 days ago by
rohitsatyam102
▴ 20
4
votes
4
replies
772
views
Surprising behavior for DEXseq dispersion estimate plot?
DESeq2
DEXSeq
6 days ago • updated 4 days ago
Elijah
• 0
0
votes
1
reply
146
views
DESeq use in lipidomic analysis
DESeq2
package
Lipidomics
updated 5 days ago by
Michael Love
43k • written 6 days ago by
泓宇
• 0
0
votes
3
replies
575
views
how many significant figures are there in DESeq2 output
DESeq2
updated 9 days ago by
ATpoint
★ 4.7k • written 11 days ago by
Matthew McCormack
▴ 180
2
votes
5
replies
963
views
Group-specific condition effects with individuals nested: matrix is not full rank
DESeq2
13 days ago • updated 12 days ago
picasa1983
• 0
3
votes
7
replies
2.0k
views
Vertical line at A=0, nothing else, on MA plot
deseq2
updated 12 days ago by
melissa
▴ 10 • written 6.0 years ago by
david.wheeler7
▴ 20
0
votes
1
reply
299
views
How to adjust for Cell Type Differences between groups in Pseudo-Bulk Differential Gene Expression analysis in scRNA-seq?
SingleCellData
DESeq2
pseudobulk
sing
updated 14 days ago by
James W. MacDonald
68k • written 14 days ago by
Sara
• 0
1
vote
1
reply
427
views
Interpreting DESEQ2 interaction term and inclusion of covariate
DESeq2
updated 15 days ago by
swbarnes2
★ 1.4k • written 15 days ago by
Len
▴ 10
1
vote
1
reply
267
views
DEseq2 design for additive treatment
DESeq2
updated 15 days ago by
ATpoint
★ 4.7k • written 16 days ago by
picasa1983
• 0
2
votes
2
replies
474
views
Plot title for PCA in DESeq2
DESeq2
17 days ago • updated 14 days ago
AL
• 0
0
votes
3
replies
528
views
Differential variance analysis - can i use deseq2
DESeq2
DifferentialExpression
RNASeq
RNASeqData
scRNAseq
updated 18 days ago by
Michael Love
43k • written 21 days ago by
Marwan
• 0
1
vote
2
replies
1.0k
views
Weird p-values using DESeq2?
DESeq2
RNASeq
updated 25 days ago by
ATpoint
★ 4.7k • written 25 days ago by
ingrid.h
• 0
0
votes
1
reply
146
views
Comparing Mock vs Infected in WT and Knockout Cells
DESeq2
DifferentialExpression
DifferentialRegulation
RNASeq
GeneSetEnrichment
updated 28 days ago by
Michael Love
43k • written 4 weeks ago by
Sahil
• 0
0
votes
3
replies
499
views
DESeq2 adujsted p-value cutoff seemingly not working
DESeq2
updated 28 days ago by
980linnet
• 0 • written 4 weeks ago by
Guilherme
• 0
0
votes
2
replies
367
views
accounting for vs. removing batch effects with DESeq2 in RNAseq analysis
DESeq2
updated 4 weeks ago by
Blair
• 0 • written 5 weeks ago by
A
• 0
0
votes
3
replies
434
views
displaying the differential expression with deseq2 over 4X2 samples with the individual changes in gene expression per sample
DESeq2
updated 14 days ago by
Leonard
• 0 • written 5 weeks ago by
Martijn
• 0
0
votes
3
replies
391
views
How to compare expression levels of genes between scRNAseq and bulkRNAseq?
Seurat
RNASeq
DESeq2
scRNAseq
updated 5 weeks ago by
jodan
• 0 • written 6 weeks ago by
Quang NN
▴ 10
0
votes
1
reply
206
views
DEG analysis across multiple timepoints, treatments and tissues
DifferentialExpression
RNASeq
DESeq2
updated 5 weeks ago by
Michael Love
43k • written 6 weeks ago by
Sim
• 0
2
votes
2
replies
460
views
Selection of correct coefficients or contrasts in DESeq2
DESeq2
updated 6 weeks ago by
swbarnes2
★ 1.4k • written 6 weeks ago by
Nathan
• 0
0
votes
4
replies
965
views
Why select top highly expressed genes based on normalized counts, but then plot VST-transformed values in DESeq2 pheatmap?
RNASeq
DESeq2
6 weeks ago
Quang NN
▴ 10
0
votes
2
replies
472
views
How to generate circular cnetplot and color nodes and edges in the same plot ?
cnetplot
DESeq2
6 weeks ago
Blandine
• 0
0
votes
5
replies
348
views
Is there a way to do DEG analysis across two batches sharing one sample only?
DESeq2
DifferentialExpression
RNASeqData
BatchEffect
written 7 weeks ago by
georgeyu703
• 0
0
votes
4
replies
498
views
How to sort negative log2FC or FC in RNAseq for GSEA?
DESeq2
GSEA
clusterProfiler
7 weeks ago • updated 6 weeks ago
Quang NN
▴ 10
2
votes
5
replies
402
views
Differential variability modelling with vst() expression
limma
DESeq2
written 8 weeks ago by
jtsy6383
• 0
0
votes
3
replies
555
views
DESeq2 analysis with transcript counts
Transcriptomics
RNASeq
DESeq2
written 8 weeks ago by
sindhuri
• 0
2
votes
9
replies
990
views
Why did PCA plots look exactly the same after adjusting for batch covariate in DESeq2 design?
DESeq2
BatchEffect
8 weeks ago • updated 7 weeks ago
Quang NN
▴ 10
1
vote
1
reply
424
views
Considerations behind the choice of RNAseq Differential Expression Analysis Tools
fishpond
DESeq2
rnaseqDTU
updated 9 weeks ago by
Michael Love
43k • written 9 weeks ago by
muhammadsohailraza
• 0
0
votes
1
reply
210
views
Want numbers in DESeq to be seen as numerical, not categorical
DESeq2
updated 9 weeks ago by
swbarnes2
★ 1.4k • written 9 weeks ago by
natalievillafranca
• 0
0
votes
1
reply
400
views
Please, I desperately need help with DESeq2. Three groups of repeated measures with two timepoints... (plus an additional group)
edgeR
DESeq2
updated 9 weeks ago by
Michael Love
43k • written 11 weeks ago by
Jimmy Mak
• 0
2
votes
1
reply
402
views
Is it possible to work with DESeq2 where data is not properly matched for sex and age
DESeq2
RNASeqData
updated 9 weeks ago by
Michael Love
43k • written 10 weeks ago by
KR
• 0
0
votes
2
replies
391
views
DESeq error: counts matrix should be numeric, currently it has mode: character
DESeq2
10 weeks ago
Kucsera
• 0
1
vote
1
reply
411
views
Can DESeq2's design compensate for sequencing experimental design shortcomings?
DESeq2
updated 11 weeks ago by
Michael Love
43k • written 3 months ago by
txema.heredia
• 0
1
vote
9
replies
924
views
Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
limma
DESeq2
edgeR
written 12 weeks ago by
anna.cot.anna.cot
▴ 30
4
votes
2
replies
495
views
Standardization of rlog transformed counts before PCA
RNASeq
DESeq2
Visualization
updated 12 weeks ago by
ATpoint
★ 4.7k • written 12 weeks ago by
bbao
▴ 10
1
vote
5
replies
643
views
Deseq2 Design
RNASeq
DESeq2
updated 12 weeks ago by
James W. MacDonald
68k • written 12 weeks ago by
pm_25
• 0
0
votes
5
replies
643
views
p value is 0, padj is 0
DESeq2
12 weeks ago
Wirayatida
• 0
1
vote
2
replies
622
views
Pre-filtering TPM kallisto for ssGSEA / DESeq2
tximport
DESeq2
GSVA
3 months ago • updated 12 weeks ago
georgii.vdovin
• 0
0
votes
7
replies
963
views
DESeq2: Gene is DEG in one experiment but not in the other depite similar baseMean, L2FC, ...
DESeq2
updated 3 months ago by
txema.heredia
• 0 • written 3 months ago by
fjrsa
• 0
0
votes
1
reply
357
views
Deseq2 Error in solve.default(qr.R(qrx)) : 'a' (1 x 0) must be square
DESeq2
updated 3 months ago by
Michael Love
43k • written 3 months ago by
bhandary.8590
▴ 20
0
votes
3
replies
498
views
DESeq2 - Comparing Comparisons
DESeq2
updated 3 months ago by
James W. MacDonald
68k • written 3 months ago by
sk
• 0
0
votes
4
replies
634
views
lfcShrink and alpha value
lfcShrink
DESeq2
3 months ago
cropero
• 0
2
votes
1
reply
394
views
DESeq2- merging technical vs biological replicates
DESeq2
RNASeqData
updated 3 months ago by
Michael Love
43k • written 3 months ago by
Nikita
• 0
0
votes
2
replies
281
views
DESeq2 dispersion estimates fitting drastically different based on explanatory variable scaling
dispersionestimates
DESeq2
updated 3 months ago by
Michael Love
43k • written 3 months ago by
Sam
• 0
0
votes
2
replies
315
views
DESeq2 sizefactors counfounded with biological treatment
DESeq2
3 months ago
Anna
• 0
1
vote
2
replies
624
views
Deseq2 res Function
DESeq2
updated 3 months ago by
James W. MacDonald
68k • written 3 months ago by
sap275
▴ 10
0
votes
4
replies
512
views
Help with RSEM counts: do I transpose matrix?
RSEM
DESeq2
3 months ago
NB
• 0
0
votes
5
replies
551
views
Identical samples after deseq2 batch effect removal
DESeq2
updated 3 months ago by
Michael Love
43k • written 3 months ago by
maripane
• 0
0
votes
1
reply
305
views
DE analysis on Single cell RNA Seq time course data (using pseudotime) with 5 different genotypes
DESeq2
updated 3 months ago by
James W. MacDonald
68k • written 3 months ago by
rj1435
• 0
1
vote
2
replies
371
views
Differene between the Wald and LRT test for time-series gene expression data
DESeq2
3 months ago
Denise
• 0
50 results • Page
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Comment: Loading Ensembl 98 EnsDb from Annotation Hub fails with database disk image malf
by
AB
▴ 110
Force=TRUE did the trick. Thank you.
Comment: www.stats.ox.ac.uk seems to be off, leading to errors/delays when installing pac
by
DENG
• 0
[https://github.com/djunsheep/CRANextra/blob/main/README.md][1] add the following to the `.Rprofile` and `Rprofile.site` # fixing the…
Comment: Transcript ID Mismatch in riboWaltz Annotation Table
by
shepherl
4.1k
riboWalze is not a Bioconductor package and does not appear to be a Bioconductor function; the maintainer is not guaranteed to be on this …
Answer: Loading Ensembl 98 EnsDb from Annotation Hub fails with database disk image malf
by
shepherl
4.1k
What is your `sessionInfo()`? I just tried locally and it works fine so there might have been a bad or interrupted download when initially…
Comment: error in ChAMP loading file
by
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• 0
Great troubleshooting steps for ChAMP import issues! "ChAMP methylation data import errors" on [Google Search][1] for similar cases. Header…
Votes
Answer: Loading Ensembl 98 EnsDb from Annotation Hub fails with database disk image malf
Answer: Differential expression of proteins using somascan data
Answer: Surprising behavior for DEXseq dispersion estimate plot?
Answer: Handle zero effective gene length when tximport RSEM results
Handle zero effective gene length when tximport RSEM results
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