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Comment: How can I generate a heatmap and clustering of differentially expressed genes in
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Renuka
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Thanks Kelvin i had same doubt .Do i need to do log2 (FPKMcounts) for this
Comment: Is there a way to do DEG analysis across two batches sharing one sample only?
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georgeyu703
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0
Michael, Thank you very much!
Comment: Is there a way to do DEG analysis across two batches sharing one sample only?
by
Michael Love
43k
Yes, with the knowledge that, it is expected to be underpowered.
Comment: Is there a way to do DEG analysis across two batches sharing one sample only?
by
georgeyu703
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0
Thank you, Michael. How? Could you provide more details? Can I just do it as usual, I mean to include batch in the model?
Answer: Is there a way to do DEG analysis across two batches sharing one sample only?
by
Michael Love
43k
The short answer is yes, you can use even a single sample to estimate the batch effect (and therefore control for it in otherwise confounde…
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