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RNASeq123
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231
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Assistance with Bulk RNA-seq Data Analysis Including Random Effect (`duplicateCorrelation` from `limma`)
limma
RNAseq123
11 weeks ago
tim.meese
• 0
1
vote
2
replies
457
views
Improving the design matrices vignette in RNAseq123
RNAseq123
8 months ago • updated 7 months ago
Lluís Revilla Sancho
▴ 760
1
vote
1
reply
643
views
Differential gene expression analysis
RNASeqData
RNAseq123
updated 12 months ago by
ATpoint
★ 4.6k • written 12 months ago by
kaurprab
• 0
0
votes
2
replies
833
views
Deseq2 paird analysis in a cohort mixed of paired and unpaired samples
DESeq2
RNAseq123
22 months ago
fatemeh.peimani
• 0
0
votes
3
replies
1.2k
views
DESeq2 Statistic - Padj NA
DESeq2
RNAseq123
rnaseq
deseq2
metatranscriptomic
updated 24 months ago by
Michael Love
43k • written 24 months ago by
irish.prg
• 0
1
vote
6
replies
4.6k
views
KEGG enrichment analysis for non model organism
RNAseq123
2.0 years ago
afsanarupa1
• 0
2
votes
5
replies
1.3k
views
Can we massively improve RNA-Seq results by slightly reducing the counts in it?
edgeR
RNAseq123
updated 2.0 years ago by
Gordon Smyth
52k • written 2.1 years ago by
Istvan Albert
▴ 50
0
votes
1
reply
2.0k
views
Confused about understanding the output and statistics of BAM file after STAR aligning
RNAseq123
VariantFiltering
Alignment
VariantAnnotation
updated 2.1 years ago by
James W. MacDonald
67k • written 2.1 years ago by
Mohamed
▴ 30
1
vote
1
reply
935
views
Confused about design for RNA seq experiment
RNAseq
ExperimentData
RNAseq123
RNA
ExperimentalDesign
updated 2.3 years ago by
ATpoint
★ 4.6k • written 2.3 years ago by
Mohamed
▴ 30
0
votes
0
replies
725
views
Plotting a line plot for genes across 4-species
RNAseq123
2.4 years ago
Mohamed
▴ 30
2
votes
1
reply
1.3k
views
Error in RNAseq123 analysis, unexpected symbol.
RNAseq123
Rna
updated 2.5 years ago by
Gordon Smyth
52k • written 2.5 years ago by
Mikołaj Mierzejewski
• 0
5
votes
6
replies
3.3k
views
Large difference in PCA plot based on LCPM (log counts per million) and VST (variance stabilizing transformations)
PCAtools
edgeR
pcaExplorer
RNASeq
RNAseq123
2.7 years ago
Alan
• 0
0
votes
1
reply
992
views
python and R
snpeff
python
tophat
r
RNAseq123
updated 3.4 years ago by
Kevin Blighe
★ 4.0k • written 3.4 years ago by
kusum
• 0
1
vote
1
reply
1.8k
views
featureCounts running too long
RNAseq123
updated 10 months ago by
difraiadomenico
• 0 • written 3.8 years ago by
demirkalecy
▴ 10
0
votes
0
replies
667
views
Circular RNA
CircularRNA
RNASeq
RNAseq123
3.9 years ago
rpokhre2
• 0
1
vote
3
replies
5.4k
views
Using TMM normalized counts in Deseq2
RNAseq123
written 3.9 years ago by
mmicky5050
• 0
12
votes
4
replies
4.8k
views
differential expression analysis of htseq data with edgeR/voom
voom
htseq
edgeR
limma
RNASeq123
updated 5.9 years ago by
Gordon Smyth
52k • written 7.5 years ago by
wd
▴ 30
17 results • Page
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Comment: Normalizing batch effects using technical replicates for DGE analysis between tw
by
anna.cot.anna.cot
▴ 30
Thank you! This is actually quite close to my real dataset. I simplified the names to make it easier as an example, but I have now correcte…
Comment: how should I apply "cpg.annotate" to TCGA methylation data in hg38 for HM450K?
by
Tim Peters
▴ 200
Hi Xiaofei, Is it possible to generate a matrix of beta values or M-values with rownames as probe IDs from this data? If so, cpg.annotate(…
Comment: Normalizing batch effects using technical replicates for DGE analysis between tw
by
Gordon Smyth
52k
That looks basically correct, although the terms that you are inputing to `makeContrasts` do not match the condition names in the targets f…
Comment: Normalizing batch effects using technical replicates for DGE analysis between tw
by
James W. MacDonald
67k
Oh, right. There's two ways to enter code. If you are in the middle of a sentence and you want to include a function name, you wrap the nam…
Comment: Normalizing batch effects using technical replicates for DGE analysis between tw
by
anna.cot.anna.cot
▴ 30
Thank you James, that really helped! I tried CODE `enter code here` but I must have dome it incorrectly, as it showed all in red.
Votes
Answer: nightmare to make custom txdb for genomes from ToxoDB
Comment: nightmare to make custom txdb for genomes from ToxoDB
Answer: Normalizing batch effects using technical replicates for DGE analysis between tw
Answer: nightmare to make custom txdb for genomes from ToxoDB
A: Advice on correcting for batch effect using DESeq2
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