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Possible ways of performing non-specific intensity filtering on Perfect-match only affymetrix array
oligo
DABG
non-specific filtering
primeview
9.6 years ago
svlachavas
▴ 840
1
vote
5
replies
2.2k
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Appropriate merge of batches of the same array to create a final total ExpressionSet
frma
bioconductor
batcheffect
Primeview
insilicomerging
updated 9.6 years ago by
Matthew McCall
▴ 830 • written 9.6 years ago by
svlachavas
▴ 840
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Comment: Crossover design with limma
by
SamGG
▴ 350
IMHO, here are the missing annotations treatmentdrug_orderDrugPlac - treatmentplacebo_orderDrugPlac, # effect of drug for the Drug…
Answer: What type of counts data to import for performing Isoform analysis in edgeR
by
ATpoint
★ 4.5k
You don't need any of these files. You need the `catchSalmon` function which reads `quant.sf` and the inferential (Bootstrap/Gibbs) replica…
Comment: makeContrastsDream for Interaction
by
abadgerw
• 0
Thank you! I will try this out.
Comment: Merge Replicates vs Mixed Model
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abadgerw
• 0
Thanks, @gabrielhoffman The data is actually proteomics so it is intensity data which is why I did not use voom but rather ran ebayes wi…
Comment: ANOVA like approach of edgeR
by
Gordon Smyth
52k
To identify common upregulated or downregulated genes, you would need to test for media condition contrasts for each cell type separately, …
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Comment: makeContrastsDream for Interaction
Answer: Limma- Print-tip loess normalisation-printer layout information does not match M
Answer: Limma- Print-tip loess normalisation-printer layout information does not match M
Comment: Limma- Print-tip loess normalisation-printer layout information does not match M
Answer: ANOVA like approach of edgeR
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