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Answer: Poor dispersion fit in DESeq2
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Michael Love
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The fit (red line) doesn't matter because you have so many samples. Note there is almost no shrinkage.
Besides that you may be intereste…
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I'd recommend actually meeting with a local statistician to work out the analysis plan, or someone familiar with linear models in R.
I h…
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> Now I am interested in comparing the DE genes between the resistant and acute groups, but I am unsure how to account for DMSO in that com…
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Tthis happens because the sequence spectraVariable is expected to be an atomic vector or a List with elements of length 1, but it's not - s…
Comment: How should I run ssGSEA Hallmark Enrichment Analysis?
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Ginny
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I think the other side of this question is that the non protein coding genes would be in the "background", they would influence the ranking…
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