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EmptyDrops
•
reset
0
votes
2
replies
581
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
8 months ago
rocanja
▴ 60
0
votes
1
reply
943
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 18 months ago by
Peter Hickey
▴ 740 • written 18 months ago by
sandra.garces.19
• 0
4
votes
5
replies
3.1k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
2.3 years ago
Peter Hickey
▴ 740
1
vote
1
reply
1.2k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 3.8 years ago by
Aaron Lun
★ 28k • written 3.8 years ago by
Peter Hickey
▴ 740
4
votes
5
replies
2.3k
views
emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 4.1 years ago by
Aaron Lun
★ 28k • written 5.2 years ago by
C T
▴ 140
5
votes
1
reply
4.9k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 2.3 years ago by
Stefano Mangiola
▴ 20 • written 5.3 years ago by
shbrief
▴ 20
0
votes
2
replies
1.3k
views
Scalability of emptyDrops() running times
dropletutils
emptydrops
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
rosano
• 0
7 results • Page
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Comment: Help with RSEM counts: do I transpose matrix?
by
NB
• 0
OK thank you.
Comment: Help with RSEM counts: do I transpose matrix?
by
James W. MacDonald
67k
You can ask here, but please read the `DESeq2` vignette first, which should answer most questions.
Comment: Help with RSEM counts: do I transpose matrix?
by
NB
• 0
Thank you. If I have questions about QC/normalization, do I ask them here or ask a new question?
Answer: Help with RSEM counts: do I transpose matrix?
by
James W. MacDonald
67k
Yes. Samples in columns and genes in rows.
Comment: Issues with BSgenome.Mmusculus.UCSC.mm39 and generating motif matrix in Signac (
by
James W. MacDonald
67k
It's not informative except for me to say I was right that it's `getSeq` that is providing the error. It appears that `AddMotifs` is passin…
Votes
Answer: Detecting genomic signatures of drug resistance breast cancer in transcriptom
Comment: Issues with BSgenome.Mmusculus.UCSC.mm39 and generating motif matrix in Signac (
Answer: Issues with BSgenome.Mmusculus.UCSC.mm39 and generating motif matrix in Signac (
Comment: does anyone how to create rarefaction curves from a phyloseq object?
Answer: ANOVA like approach of edgeR
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