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EdgeR
•
reset
0
votes
3
replies
154
views
DiffBind edgeR normalization --- why are the normalization factors all equal to 1?
edgeR
DiffBind
updated 2 hours ago by
ATpoint
★ 4.7k • written 1 day ago by
tifaGis
• 0
2
votes
4
replies
464
views
EdgeR Biological CVs
edgeR
updated 5 weeks ago by
Gordon Smyth
52k • written 5 weeks ago by
James
• 0
3
votes
5
replies
1.1k
views
Differential expression of protein coding ENSGs (Ensembl IDs) | When to biomaRt?
limma
edgeR
biomaRt
updated 6 weeks ago by
James W. MacDonald
68k • written 6 weeks ago by
Jonathan
▴ 10
0
votes
2
replies
307
views
Mean variance curve troubleshoot filtering
limmavoom
filtering
edgeR
RNASeqData
meanvariancetrend
7 weeks ago
gingergeiger22
• 0
0
votes
1
reply
388
views
Please, I desperately need help with DESeq2. Three groups of repeated measures with two timepoints... (plus an additional group)
edgeR
DESeq2
updated 8 weeks ago by
Michael Love
43k • written 9 weeks ago by
Jimmy Mak
• 0
1
vote
8
replies
976
views
Incorporating Salmon offset matrix into Wilcoxon DEG analysis
edgeR
8 weeks ago • updated 7 weeks ago
Andre
• 0
1
vote
9
replies
905
views
Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
limma
DESeq2
edgeR
written 10 weeks ago by
anna.cot.anna.cot
▴ 30
6
votes
10
replies
2.7k
views
What type of counts data to import for performing Isoform analysis in edgeR
limma
isoform
edgeR
salmon
R
updated 11 weeks ago by
ATpoint
★ 4.7k • written 3 months ago by
mohammedtoufiq91
▴ 10
7
votes
6
replies
1000
views
ANOVA like approach of edgeR
edgeR
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
Talip
▴ 10
3
votes
3
replies
619
views
Is it recommended to analyze different tissues together when using edgeR
DifferentialExpression
edgeR
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
Lars Roed
• 0
2
votes
4
replies
1.8k
views
Differential Expression Analysis with unbalanced batches without replication between conditions
edgeR
BatchEffect
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
Thili
• 0
1
vote
8
replies
1.4k
views
Combining RNAseq and microarray data using edgeR/limma-voom
limma
voom
RNASeq
edgeR
Microarray
written 3 months ago by
Adam Marstrand
• 0
0
votes
1
reply
559
views
single cell RNA seq pseudobulk batch correction
edgeR
updated 3 months ago by
James W. MacDonald
68k • written 3 months ago by
mairahtkhan
• 0
3
votes
3
replies
1.0k
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 4 months ago by
Satoshi
• 0
0
votes
4
replies
713
views
RNA-seq: samples form different experiments, how to do DGEs?
DGEs
edgeR
RNASeqData
4 months ago
Philippine
• 0
4
votes
6
replies
1.3k
views
Zero intra-block correlation in RRBS analysis using voomLmFit with block
limma
duplicateCorrelation
edgeR
modelMatrixMeth
voomLmFit
4 months ago
altintas.ali
• 0
1
vote
2
replies
395
views
edgeR 4.2.2 not compiled for Windows
edger
edgeR
4 months ago
Hongjian
• 0
3
votes
3
replies
400
views
How to subset voomLmFit (MArrayLM) object?
limma
edgeR
4 months ago
Guido Hooiveld
★ 4.1k
4
votes
1
reply
424
views
edgeR 4.2.1 compression bug: a minimally reproducible snippet
edger
edgeR
updated 4 months ago by
Gordon Smyth
52k • written 4 months ago by
Hongjian
• 0
1
vote
1
reply
324
views
Normalization RNA-seq for multiple plates
Normalization
DESeq2
RNASeqData
edgeR
updated 5 months ago by
ATpoint
★ 4.7k • written 5 months ago by
Pimpa
• 0
1
vote
2
replies
573
views
Poor dispersion fit in DESeq2
limma
DESeq2
RNASeq
edgeR
updated 5 months ago by
Michael Love
43k • written 5 months ago by
Ben
▴ 10
0
votes
2
replies
478
views
EdgeR - Apparent Wrong Direction of logFC with Low Count Data
limma
edgeR
updated 5 months ago by
Gordon Smyth
52k • written 5 months ago by
blmurphy
• 0
1
vote
2
replies
415
views
Inconsistent result in edgeR
edgeR
5 months ago
hiroko.ohmiya
• 0
1
vote
1
reply
549
views
Help with design matrix for RNA-seq data using edgeR
edgeR
RNASeqData
updated 5 months ago by
Gordon Smyth
52k • written 5 months ago by
erik.burchard
• 0
2
votes
9
replies
964
views
Seeking Advice on Using edgeR and variancePartition for RNA-seq Data from Multiple Tissues and Patients
edgeR
variancePartition
updated 6 months ago by
Gordon Smyth
52k • written 6 months ago by
Seymoo
• 0
0
votes
3
replies
654
views
comparing common differentially expressed genes from two Rna-Seq Dataset.
DESeq2
edgeR
DifferentialExpression
written 6 months ago by
dancingcow
• 0
1
vote
1
reply
1.1k
views
Expected memory usage for analyzing large single-cell seq datasets (> 450,000 cells)?
BigData
Clustering
memory
SingleCellExperiment
edgeR
updated 6 months ago by
Aaron Lun
★ 28k • written 6 months ago by
Joseph
• 0
0
votes
1
reply
457
views
edgeR - design a complex matrix
design
model.matrix
edgeR
complexdesign
updated 6 months ago by
Gordon Smyth
52k • written 6 months ago by
Karla
• 0
3
votes
3
replies
672
views
Optimizing CPM Calculation for Varied RNA-seq Data
edgeR
updated 6 months ago by
James W. MacDonald
68k • written 6 months ago by
Ahdee
▴ 50
0
votes
4
replies
704
views
DE analysis in EdgeR /Can any one please help me.
RNASeqData
DEGraph
RNASeq
edgeR
DegNorm
written 7 months ago by
Shaozhen
• 0
1
vote
8
replies
1.2k
views
Advice on appropriateness of an offset in glmGamPoi/edgeR
DESeq2
glmGamPoi
offset
edgeR
scRNAseq
updated 7 months ago by
Gordon Smyth
52k • written 8 months ago by
Alec
• 0
3
votes
5
replies
2.2k
views
Right way to pseudobulk donors across replicates in edgeR
pseudobulk
edgeR
DifferentialExpression
updated 8 months ago by
Reza Ghamsari
• 0 • written 12 months ago by
Jack S.
▴ 60
0
votes
4
replies
729
views
Single Cell Experiment : Differential Expression "List of length 0" - Help!
DifferentialExpression
scran
scater
SingleCellExperiment
edgeR
8 months ago
vohara4
• 0
3
votes
6
replies
944
views
Comapre two groups in edgeR- Coefficient and logFC explanation
edgeR
8 months ago
JG13
• 0
3
votes
2
replies
793
views
Identifying DEGs using limma - NanoString GeoMx-NGS RNA Expression Data
NanoStringNCTools
limma
ExpressionData
RNASeq
edgeR
9 months ago
mohammedtoufiq91
▴ 10
3
votes
3
replies
852
views
How to correctly adjust for gender in edgeR
design
glm
edgeR
updated 9 months ago by
Gordon Smyth
52k • written 9 months ago by
Guillermo
• 0
1
vote
3
replies
753
views
How to approach time course RNAseq data? Seeing 80% DE genes in timepoint groups
DifferentialExpression
TimeCourse
timeOmics
edgeR
RNAseq
updated 9 months ago by
Gordon Smyth
52k • written 9 months ago by
Ashley
• 0
0
votes
1
reply
452
views
NEED FOR NESSASORY HELP
RNASeqData
edgeR
updated 10 months ago by
Gordon Smyth
52k • written 10 months ago by
z_bahari
• 0
4
votes
2
replies
1.3k
views
Why does GSEA on edgeR results for randomized samples give highly significant p-values?
fgsea
edgeR
10 months ago • updated 9 months ago
gene_bioconductor
▴ 10
1
vote
2
replies
1.0k
views
Citation for edgeR user guide
edgeR
written 10 months ago by
k___
▴ 30
4
votes
6
replies
1.2k
views
FilterByExpr low counts with small sample size
edgeR
10 months ago
Jonathan
▴ 10
0
votes
2
replies
456
views
How to make DGEList and design matrix by edgeR package
DGEList
edgeR
10 months ago
prity6459
• 0
0
votes
3
replies
689
views
Finding DEGs by using edgeR
subsettingdata
edgeR
designmatrix
updated 10 months ago by
Gordon Smyth
52k • written 10 months ago by
prity6459
• 0
5
votes
3
replies
1.1k
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 9 months ago by
Gordon Smyth
52k • written 10 months ago by
Shaimaa Gamal
• 0
3
votes
3
replies
1.2k
views
permutation test in edgeR
rna-seq
edgeR
updated 10 months ago by
Gordon Smyth
52k • written 10 months ago by
Netanel
• 0
1
vote
3
replies
726
views
Why is my gene not significantly differentially expressed?
limmaGUI
edgeR
limma
updated 10 months ago by
Gordon Smyth
52k • written 10 months ago by
Simran
• 0
5
votes
6
replies
1.0k
views
Not fully paired differential gene expression analysis
edgeR
DifferentialExpression
updated 10 months ago by
ATpoint
★ 4.7k • written 10 months ago by
nhaus
▴ 70
1
vote
3
replies
632
views
Lib sizes in the DGE object are different from original lib sizes
edgeR
DifferentialExpression
updated 10 months ago by
James W. MacDonald
68k • written 10 months ago by
Shaimaa Gamal
• 0
0
votes
2
replies
636
views
edgeR analysis: glm functions and offset matrix
tximport
edgeR
Normalization
11 months ago
Marianna
▴ 20
0
votes
4
replies
1.0k
views
Adjust RNA-seq counts (more than one batch variable) for plotting differentially expressed genes
ComBat
sva
edgeR
11 months ago
Lucy
▴ 60
50 results • Page
1 of 1
Recent ...
Replies
Comment: DiffBind edgeR normalization --- why are the normalization factors all equal to
by
ATpoint
★ 4.7k
No, normalization factors from edgeR rather than size factors from DESeq2 are not proportional, it's a different concept and implementation…
Answer: how many significant figures are there in DESeq2 output
by
ATpoint
★ 4.7k
I strongly recommend to not concern yourself with these sorts of marginal problems because you're entering a realm in which you're entirely…
Comment: Problem using locateVariants() from the VariantAnnotation package
by
Marco
• 0
Ok, I think I found the problem. By looking at the `locateVariants()` source code, I was able to pinpoint that the problems is when trying …
Comment: Conumee for EPIC and EPICv2 copy number
by
Adam
• 0
As described on the [github](https://github.com/hovestadtlab/conumee2) the way to do this is using array_type = c("EPIC", "EPICv2") This i…
Comment: graper 1.22 Build is Broken
by
shepherl
4.1k
This is not an appropriate place to report this. this is for usage questions. maintainers are notified automatically when it is failing on …
Votes
A: Unbalanced experiment with multiple samples from each patient.
Unbalanced experiment with multiple samples from each patient.
Answer: Vertical line at A=0, nothing else, on MA plot
Answer: GSVA Error Code "Calling gsva(expr=., gset.idx.list=., method=., ...) is defunct
Answer: Group-specific condition effects with individuals nested: matrix is not full ra
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