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Showing :
Contrasts
•
reset
3
votes
3
replies
613
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 7 months ago by
ATpoint
★ 4.6k • written 7 months ago by
bioinf
▴ 10
2
votes
3
replies
614
views
edgeR, 3 x 2 design with a batch effect and contrasts
design
edgeR
contrasts
updated 9 months ago by
Gordon Smyth
52k • written 9 months ago by
JKim
• 0
0
votes
3
replies
1.1k
views
Multiple Testing When Using Multiple Contrasts/Comparisons in DESEQ2
MultipleComparison
DESeq2
Genetics
contrasts
RNASeq
updated 17 months ago by
James W. MacDonald
67k • written 17 months ago by
a26fd14e
• 0
1
vote
2
replies
741
views
Individual DESeq2 runs versus a single DESeq2 run with contrasts produce different results
contrasts
DESeq2
18 months ago
jovel_juan
▴ 30
3
votes
6
replies
1.4k
views
Question about making Contrasts for comparisons and assignment of values
edgeR
contrasts
limma
design
model.matrix
2.0 years ago
mohammedtoufiq91
▴ 10
1
vote
3
replies
862
views
Limma F-statistic identical for different comparison, why?
limma
F-statistic
contrasts
updated 2.1 years ago by
Gordon Smyth
52k • written 2.1 years ago by
jeltje.van.baren
• 0
3
votes
8
replies
4.4k
views
DESeq2 how to specify contrast to test difference of differences
DifferentialExpression
contrasts
interaction
DESeq2
written 2.6 years ago by
Nicole
▴ 10
0
votes
3
replies
1.7k
views
limma voom contrasts
contrasts
limma
voom
updated 2.8 years ago by
Gordon Smyth
52k • written 2.8 years ago by
S T
▴ 90
0
votes
1
reply
903
views
DESeq2 2x3 design contrast question
DESeq2
contrasts
updated 2.9 years ago by
Michael Love
43k • written 2.9 years ago by
Mir
• 0
0
votes
2
replies
1.2k
views
limma voom; fitted lines, factors that aren't of interest, and hypothesis testing
topTable
voom
contrasts
3.3 years ago
James
• 0
0
votes
3
replies
1.3k
views
DESeq2: difficulty with contrasts and main effects
DESeq2
phyloseq
contrasts
updated 3.4 years ago by
Kevin Blighe
★ 4.0k • written 3.4 years ago by
Mir
• 0
2
votes
8
replies
2.5k
views
DESeq2 numbers of DEGs not consistent with PCA plot
DifferentialExpression
DESeq2
contrasts
3.6 years ago
Jane
▴ 10
3
votes
8
replies
1.5k
views
Same contrasts returning different edgeR results
edger
limma
contrasts
DGE
updated 5.0 years ago by
James W. MacDonald
67k • written 5.0 years ago by
s.connell
• 0
0
votes
1
reply
1.2k
views
DESeq2 - how to run contrasts leaving out one factor, not just using the reference?
deseq2
contrasts
reference level
updated 5.0 years ago by
Michael Love
43k • written 5.0 years ago by
ananda2
• 0
0
votes
0
replies
842
views
EdgeR: Transforming coefficients before testing for differential expression
edger
contrasts
model.matrix
circadian
5.0 years ago
ingerslev
• 0
0
votes
1
reply
1.3k
views
DESeq2 very complex design - how extract contrasts of interest?
DESeq2
RNA-Seq
Multifactorial Design
GLM
Contrasts
updated 5.1 years ago by
Michael Love
43k • written 5.1 years ago by
ananda2
• 0
2
votes
3
replies
1.6k
views
DESeq2 2x2 factorial design: find genes unique to factor A?
deseq2
deseq
differential expression
contrasts
updated 5.1 years ago by
Michael Love
43k • written 5.1 years ago by
Keith Hughitt
▴ 180
0
votes
4
replies
1.2k
views
Setting up contrasts with 'limma', patient data, small number of repeats
limma
nanostring
contrasts
updated 5.2 years ago by
Gordon Smyth
52k • written 5.2 years ago by
uridavid.akavia
• 0
0
votes
1
reply
830
views
DESeq2 compairson of 3 samples from 1 type
deseq2
multiple factor design
contrasts
experimental design
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
kehoe
• 0
3
votes
5
replies
1.7k
views
Experimental design RNAseq differential expression
edger
limma
design
contrasts
batch
updated 5.8 years ago by
Aaron Lun
★ 28k • written 5.8 years ago by
Pietro
▴ 40
2
votes
6
replies
1.3k
views
Making sense out of makeContrasts and using coefficents for contrasts
edger
contrasts
updated 5.8 years ago by
Gordon Smyth
52k • written 5.8 years ago by
Maiv
• 0
21 results • Page
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Recent ...
Replies
Comment: Cross-validation with multiple control subgroups in limma
by
Ali Barry
▴ 40
Thanks so much for taking the time here, the insights are much appreciated. I suspected I'd have to ad hoc compare results if I did repeat …
Answer: Cross-validation with multiple control subgroups in limma
by
Gordon Smyth
52k
limma doesn't do cross-validation or resampling. Like most classical linear modeling procedures in statistics, limma uses a statistical mod…
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
by
Robert Castelo
★ 3.4k
Hi Hervé, thank you very much for your input into how to properly build and manage TxDb objects. gDNAx has two main inputs, one or more BAM…
Comment: Biostrings: Error writing long reads (>200 kbps) with writeQualityScaledXStringS
by
Aidan
▴ 60
Quick update: this is fixed in a branch awaiting PR to Biostrings (https://github.com/Bioconductor/Biostrings/pull/122). Should be in `deve…
Answer: Can DESeq2's design compensate for sequencing experimental design shortcomings?
by
Michael Love
43k
For statistical analysis plans, I recommend working with a local statistician or someone familiar with linear models in R. I have to reserv…
Votes
C: edgeR and lack of counts ID on CPM matrix
A: DESeq2 Following RSEM
A: importing RSEM data into DESeq2
A: Expected counts from RSEM in DESeq2
A: Using RSEM reads for DESeq2
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