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10x
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Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
10 months ago
rocanja
▴ 60
0
votes
1
reply
1.0k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 20 months ago by
Peter Hickey
▴ 750 • written 20 months ago by
sandra.garces.19
• 0
0
votes
2
replies
943
views
Compare and filter three BAM files
RNAseq
10X
22 months ago
wt215
• 0
4
votes
5
replies
3.3k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
2.5 years ago • updated 2.4 years ago
Peter Hickey
▴ 750
1
vote
1
reply
1.3k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 4.0 years ago by
Aaron Lun
★ 28k • written 4.0 years ago by
Peter Hickey
▴ 750
5
votes
3
replies
1.7k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 4.5 years ago by
Michael Love
43k • written 4.5 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.0k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
jma1991
▴ 70
7
votes
12
replies
8.0k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
xingxd16
▴ 20
8 results • Page
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Comment: How to sort negative log2FC or FC in RNAseq for GSEA?
by
alserg
▴ 280
`NES > 0` means that your gene set has more genes with positive logFC/stat. In your case these genes should be up in Subset1. However, if y…
Answer: affymetrix
by
James W. MacDonald
67k
When posting it helps to think about the people you are asking for help. Your post is hidden within the dummy text that comes up when you i…
Answer: Problems with GSEA
by
Guido Hooiveld
★ 4.1k
The error `Duplicate values in names(stats) not allowed` (from `fgsea` that is used under the hood) shows that the input is not unique; in …
Answer: Error compiling Rbowtie2 in R-4.4 (Bioconductor 3.20 and probably 3.19)
by
ja.garcia
• 0
It has been fixed by the Rbowtie2 maintainers in version 2.12.2. Thank you for the quick fix!
Comment: contrast matrix design for a continuous variable
by
dtzhu337
• 0
Hi Gordon, Thank you very much for your answer. Actually the variable X is the age, which I am also interested in. Somehow, age and V1 are…
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Answer: Error in download.file for TCGAutils::symbolsToRanges
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