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10x
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0
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593
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Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
8 months ago
rocanja
▴ 60
0
votes
1
reply
952
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 19 months ago by
Peter Hickey
▴ 740 • written 19 months ago by
sandra.garces.19
• 0
0
votes
2
replies
908
views
Compare and filter three BAM files
RNAseq
10X
20 months ago
wt215
• 0
4
votes
5
replies
3.2k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
2.3 years ago
Peter Hickey
▴ 740
1
vote
1
reply
1.2k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 3.9 years ago by
Aaron Lun
★ 28k • written 3.9 years ago by
Peter Hickey
▴ 740
5
votes
3
replies
1.6k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 4.4 years ago by
Michael Love
43k • written 4.4 years ago by
vincent.croset
▴ 20
4
votes
2
replies
2.9k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 5.6 years ago by
Aaron Lun
★ 28k • written 5.6 years ago by
jma1991
▴ 70
7
votes
12
replies
7.9k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
xingxd16
▴ 20
8 results • Page
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Answer: scuttle::perFeatureQCMetrics runs very slow for big scRNA-seq data
by
Aaron Lun
★ 28k
You don't mention how your matrix data is being represented, but if it's a file-backed matrix (e.g., `HDF5Matrix` or one of its variants, b…
Comment: contrast matrix design for a continuous variable
by
dtzhu337
• 0
Thank you, Gordon. X is time splines for time point, and yes, it's needed. However, I was also thinking to incorporate time point with se…
Answer: contrast matrix design for a continuous variable
by
Gordon Smyth
52k
Yes, the design and contrasts are correct. Your analysis is also adjusted for covariates defined by X, which I assume is what you want to …
Comment: fail to use addPerROIQC in Rmd
by
liu.n
• 0
That's odd. So it can run successfully within markdown but would be failed when knitting?
Comment: fail to use addPerROIQC in Rmd
by
joseph.joonsang
• 0
> dim(spe) [1] 19962 425
Votes
Answer: contrast matrix design for a continuous variable
A: What's the difference between p.adjust and qvalue?
Answer: DESeq2- merging technical vs biological replicates
Comment: What type of counts data to import for performing Isoform analysis in edgeR
Answer: What type of counts data to import for performing Isoform analysis in edgeR
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