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Answer: displaying the differential expression with deseq2 over 4X2 samples with the in
by
James W. MacDonald
67k
If you have paired data you should block on subject, which is algebraically the same as fitting a model on the difference between treated a…
Answer: How to compare expression levels of genes between scRNAseq and bulkRNAseq?
by
jodan
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0
Your approach sounds solid! You might also consider plotting a scatter plot comparing log2 fold changes from both datasets to identify gene…
Answer: Unused arguments error without used arguments in GSVA?
by
jodan
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0
It looks like the ssgseaParam function might not be passing the correct parameters to GSVA::gsva(). The error suggests that minSize and max…
Answer: Using genomicRanges / plyranges to calculate score of peaks within intervals
by
Michael Love
43k
This should be possible with plyranges using `join_overlap` and `group_by` on the metadata from the tile. E.g. create `tile.id` as a variab…
Answer: Reducing and aggregating GRanges with gaps using plyranges
by
Michael Love
43k
I added `min.gapwidth` to `reduce_ranges()` in recent plyranges releases:
https://github.com/tidyomics/plyranges/pull/105
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