Hi, I am looking to use Bioconductor subSeq to perform a depth sufficiency analysis on RNAseq counts on a sample I have. The issue I am running into is that I only have one sample (as this sample of total RNAseq is performed by mistakes), and subSeq's subsampling seems to require both the 'method' for DE analysis and the 'treatment' conditions - which is not applicable here.

I am new to R and bioinformatics altogether, but if there is a way to use subSeq only for subsampling and drawing the rarefaction curve or another method, please let me know.

Thank you