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efratsh
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@efratsh-20531
Last seen 3.8 years ago
Hello, I got unexpected results from resultsNames:
dds_taxa2 <- DESeqDataSetFromMatrix(countData = taxa_count,
colData = md_RatTime,
design= ~ RatTime)
dds_taxa2 = DESeq(dds_taxa2)
dds_taxa2$RatTime <- relevel(dds_taxa2$RatTime, ref = "BER_T0")
resultsNames(dds_taxa2)
md_RatTime
RatTime
BEP_T0_10N BEP_T0
BEP_T0_11N BEP_T0
BEP_T0_12B BEP_T0
BEP_T0_16B BEP_T0
BEP_T0_16N BEP_T0
BEP_T0_18B BEP_T0
BEP_T0_1B BEP_T0
BEP_T0_20B BEP_T0
BEP_T0_21B BEP_T0
BEP_T0_2N BEP_T0
BEP_T0_3B BEP_T0
BEP_T0_8N BEP_T0
BEP_T1_10N BEP_T1
BEP_T1_11N BEP_T1
BEP_T1_12B BEP_T1
BEP_T1_16B BEP_T1
BEP_T1_16N BEP_T1
BEP_T1_18B BEP_T1
BEP_T1_1B BEP_T1
BEP_T1_20B BEP_T1
BEP_T1_21B BEP_T1
BEP_T1_2N BEP_T1
BEP_T1_3B BEP_T1
BEP_T1_8N BEP_T1
BER_T0_10B BER_T0
BER_T0_11B BER_T0
BER_T0_12N BER_T0
BER_T0_13B BER_T0
BER_T0_17N BER_T0
BER_T0_19B BER_T0
BER_T0_1N BER_T0
BER_T0_21N BER_T0
BER_T0_23B BER_T0
BER_T0_6N BER_T0
BER_T0_7B BER_T0
BER_T0_9B BER_T0
BER_T1_10B BER_T1
BER_T1_11B BER_T1
BER_T1_12N BER_T1
BER_T1_13B BER_T1
BER_T1_17N BER_T1
BER_T1_19B BER_T1
BER_T1_1N BER_T1
BER_T1_21N BER_T1
BER_T1_23B BER_T1
BER_T1_6N BER_T1
BER_T1_7B BER_T1
resultsNames(dds_taxa2)
[1] "Intercept" "RatTime1" "RatTime2" "RatTime3"
design(dds_taxa2)
~RatTime
levels(dds_taxa2$RatTime)
[1] "BER_T0" "BEP_T0" "BEP_T1" "BER_T1"
I expect to get RatTime_BER_T1_vs_BER_T0, RatTime_BEP_T1_vs_BEP_T0, RatTime_BER_T1_vs_BEP_T1 How can I chage what I did to get it? Thank you.
sessionInfo( )
R version 3.6.1 (2019-07-05)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows >= 8 x64 (build 9200)
Matrix products: default
locale:
[1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United States.1252 LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C LC_TIME=English_United States.1252
attached base packages:
[1] grid parallel stats4 stats graphics grDevices utils datasets methods base
other attached packages:
[1] microbiome_1.8.0 ggpubr_0.4.0 gridExtra_2.3 pheatmap_1.0.12
[5] reshape2_1.4.4 PairedData_1.1.1 mvtnorm_1.1-1 gld_2.6.2
[9] MASS_7.3-51.4 factoextra_1.0.7 openxlsx_4.1.5 DESeq2_1.26.0
[13] SummarizedExperiment_1.16.1 DelayedArray_0.12.3 BiocParallel_1.16.6 matrixStats_0.56.0
[17] Biobase_2.42.0 GenomicRanges_1.38.0 GenomeInfoDb_1.22.1 IRanges_2.20.2
[21] S4Vectors_0.24.4 BiocGenerics_0.32.0 RColorBrewer_1.1-2 psych_2.0.7
[25] vegan_2.5-6 lattice_0.20-38 permute_0.9-5 phyloseq_1.30.0
[29] ggrepel_0.8.2 ggthemes_4.2.0 ggplot2_3.3.2 stringr_1.4.0
[33] tibble_3.0.3 readxl_1.3.1 taxa_0.3.4 tidyr_1.1.1
[37] dplyr_1.0.1 plyr_1.8.6 readr_1.3.1
Thank you, but even without the relevel, I did not get an expected results names.
What are the levels of RatTime here? I'm not sure why it's not giving the "B_vs_A" style of results names. Maybe something about the levels you are passing to DESeq2.
I'm shocked, I restarted the R session, and now it behaves as expected: