Entering edit mode
Dear all,
I have used DESeq2 for my Differential Expression Analysis and with
~batch + gender
I controlled for the different batches.
Now I would like to control not only for gender instead also for another variable. Can I control for 2 different variables in the model? Or do I manually need to remove it before through maybe "remove batch effect" functions?
Thank you for any hint!!
Alternatively, for questions that are not purely technical and therefore do not require the developer's "how-it-works-under-the-hood"-expertise you can post at biostars.org, it is a general community for everything related to bioinformatics. If you do please add the necessary details (this goes for any community). In your case that would be a table listing which samples belongs to which group(s) to see whether covariates (=variables) can be meaningfully modelled or are nested with each other.