I am a beginner in the programming area and I would like to ask for your help. I want to perform the deferential expression analysis using the DESeq2 package. I want to compare the expression of smallRNAs in three treated samples vs three control samples. I have the results from smallRNA-seq in txt files each for every sample, formated like this: sRNA_sequence raw_count TTTGGATTGAAGGGAGCTCTA 59493 Could you tell me how to prepare the file (dds) for the DESeq2 analysis?