I am looking for details on the principle and recommended workflow using the catchSalmon
and catchKallisto
functions for differential tx-level analysis with edgeR
. The manual mentions but does not offer any details on the functions.
help()
mentions that a per-transcript overdispersion value is estimated which is then used to divide the transcript counts by.
Is that all one has to do, followed by the standard (e.g. QLF) workflow?
Is there any benchmarking or performance data available on how the method compares to other approaches such as swish
or ballgown
or sleuth
?
I see, thank you for the response!
A preprint on catchSalmon with extensive comparisons is finally available: Baldoni PL, Chen Y, Hediyeh-zadeh S, Liao Y, Dong X, Ritchie ME, Shi W, Smyth GK (2023). Dividing out quantification uncertainty allows efficient assessment of differential transcript expression in edgeR. bioRxiv https://doi.org/10.1101/2023.04.02.535231.