Hi,

I just tried to load my MAF file with maftools, but although the vignette states that the required fields are only 7 (Hugo_Symbol, Chromosome, Start_Position, End_Position, Variant_Classification, Variant_Type and Tumor_Sample_Barcode), I got the following error messages:

Error in validateMaf(maf = maf, isTCGA = isTCGA, rdup = removeDuplicatedVariants,  :
  missing required fields from MAF: Reference_Allele

Error in validateMaf(maf = maf, isTCGA = isTCGA, rdup = removeDuplicatedVariants,  :
  missing required fields from MAF: Tumor_Seq_Allele2

Could you please make these two fields (Reference_Allele and Tumor_Seq_Allele2) not required, to be coherent with the package vignette?

Thank you!

Silvia

Hi Silvia,

Reference_Allele and Tumor_Seq_Allele2 are essential fields for some of the analysis such as signature and APOBEC enrichment estimation. Apologies on my part, I will update the vignette. But for now, you can just add two columns to your MAF file (you can populate them with NA or leave them blank) with title `Reference_Allele` and `Tumor_Seq_Allele2` and it should work fine (except for signature analysis). Hope this helps.

-Anand.