I'm working on gating my raw FCS files. I have successfully created a template that gates for monocytes. Now I am trying to identify HLA-DR+ monocytes from the previously gated monocyte population. I would like to add a mindensity gate around 10^2 to a monocyte plot (BL4-H x SSC-H), but in R the openCyto program keeps creating two populations that I've listed in my dims column of the gating template (see below). I was wondering if you could help me figure out why. I've attached my gating template for reference as well. If you could help me overcome this obstacle that would be of extreme help. Thank you.
> gt<-gatingTemplate("/Users/justinyi/Desktop/gating_template.csv")
expanding pop: HLA-DR+
Adding population:monocyte
Adding population:BL4-H+
Adding population:SSC-H+
Adding population:HLA-DR
|
alias |
pop | parent | dims | gating_method | gating_args | collapseDataForGating | groupBy | preprocessing_method | preprocessing_args |
| monocyte | monocyte | root | FSC-H,SSC-H | flowClust | K=3,target=c(1e6,2e5),quantile=0.25 | NA | NA | NA | NA |
| HLA-DR | HLA-DR+ | monocyte | BL4-H,SSC-H | mindensity | gate_range=c(1.5e1,1.5e4) | NA | NA | NA | NA |
