Entering edit mode
justinyi10
▴
10
@justinyi10-13409
Last seen 7.4 years ago
Hello,
I am trying to gate for monocytes in my plot of cells (FSC-H x SSC-H) using flowClust. The center generally lies around (8e5,5e5). This gets applied to a few samples very accurately, but in others it is very off. Here is my gating template. In essence, how do I improve the accuracy of flowClust?
| alias | pop | parent | dims | gating_method | gating_args | collapseDataForGating | groupBy | preprocessing_method | preprocessing_args |
| monocytes | monocytes | root | FSC-H, SSC-H | flowClust | K=3,target=c(8e5,5e5), quantile=0.4 | NA | NA | NA | NA |
Can you provide the plots (from plotGate) to see why/how it failed?