Hi, 

I need to get the raw data (raw measurement values) for single parameter from my 11 parameters FCS file. 
How can I do this? 

Thank you.
Eva
 

Did you already see the function read.FCS in the flowCore package?

Yes, I can read the file. 
I just needed to convert it to the data frame, but I had to do something wrong, because it did not work. (I have no idea what was wrong.)
I started R again and here it is. 

S1 <- read.FCS ("Desktop/R/06_1.fcs", transformation = "scale")
S1.df <- exprs(S1)

Thanks.