how-to-normalize-single-channel-data-in-cDNA-experiment
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@vijayaraj-nagarajan-1188
Last seen 10.2 years ago
hi i am interested in comparing the channel-1 data of two different cDNA experiments that we have carried out, without considering the channel-2 data. could anyone tell me about how to normalize this single channel data alone. thanks sincerely vijayaraj nagarajan graduate student the university of southern mississippi ms, us of a
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@adaikalavan-ramasamy-675
Last seen 10.2 years ago
Why do you want to do this ? There are good reasons for using both channels in two-colour data (to account for different amounts of probe spotted, cross hybridisation and other biases at chip level). Regards, Adai On Mon, 2005-07-18 at 08:29 -0700, vijayaraj nagarajan wrote: > hi > i am interested in comparing the channel-1 data of two > different cDNA experiments that we have carried out, > without considering the channel-2 data. > could anyone tell me about how to normalize this > single channel data alone. > > thanks > sincerely > > vijayaraj nagarajan > graduate student > the university of southern mississippi > ms, us of a > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor >
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