Differential expression after different normalization methods
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Bio_Ram • 0
@bio_ram-12851
Last seen 6.1 years ago

Is it possible to use only the differential expression module of Deseq2 or any other method for miRNA isoforms expression?

I have normalized my data using RPM based normalization and looking to check the differential expression and log fold change. is there any methods so that i can test with more than one statistics to strengthen my analysis.

Thanks in Advance

 

deseq2 differential expression • 919 views
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@mikelove
Last seen 23 hours ago
United States

Yes, I have heard of DESeq2 being used for differential microRNA expression, however, you should use counts instead of normalized expression values. This is discussed in the vignette.

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