I am following the scater workshop tutorial found here:
https://github.com/hemberg-lab/scRNA.seq.course/blob/master/13-exprs-norm.Rmd
I have run all of the code from the first 12 chapters without any problem.
When I try to run this section of code:
plotRLE(
umi.qc[endog_genes, ],
exprs_mats = list(Raw = "log2_counts", CPM = "exprs"),
exprs_logged = c(TRUE, TRUE),
colour_by = "batch"
)
I get this error,
Error: could not find function "plotRLE"
To reproduce the error, i tried in a new session:
#############
library(scater, quietly = TRUE)
scater::newSCESet()
#Error in scater::newSCESet() :
# Require at least one of exprsData, tpmData, fpkmData or countData arguments.
scater::plotRLE()
#Error: 'plotRLE' is not an exported object from 'namespace:scater'
################
even though plotRLE is listed as a function here:
http://bioconductor.org/packages/release/bioc/manuals/scater/man/scater.pdf
Any help or hints appreciated
Ian
sessionInfo() R version 3.3.2 (2016-10-31) Platform: x86_64-pc-linux-gnu (64-bit) Running under: Ubuntu 16.04.1 LTS locale: [1] LC_CTYPE=en_GB.UTF-8 LC_NUMERIC=C LC_TIME=en_GB.UTF-8 LC_COLLATE=en_GB.UTF-8 LC_MONETARY=en_GB.UTF-8 LC_MESSAGES=en_GB.UTF-8 [7] LC_PAPER=en_GB.UTF-8 LC_NAME=C LC_ADDRESS=C LC_TELEPHONE=C LC_MEASUREMENT=en_GB.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] parallel stats graphics grDevices utils datasets methods base other attached packages: [1] scater_1.2.0 ggplot2_2.2.1 Biobase_2.34.0 BiocGenerics_0.20.0 loaded via a namespace (and not attached): [1] Rcpp_0.12.10 vipor_0.4.5 plyr_1.8.4 zlibbioc_1.20.0 viridis_0.4.0 bitops_1.0-6 tools_3.3.2 [8] biomaRt_2.30.0 digest_0.6.12 rhdf5_2.18.0 lattice_0.20-35 RSQLite_1.1-2 memoise_1.1.0 tibble_1.3.0 [15] gtable_0.2.0 viridisLite_0.2.0 shiny_1.0.3 DBI_0.6-1 beeswarm_0.2.3 gridExtra_2.2.1 stringr_1.2.0 [22] dplyr_0.5.0 S4Vectors_0.12.2 IRanges_2.8.2 stats4_3.3.2 locfit_1.5-9.1 grid_3.3.2 shinydashboard_0.5.3 [29] data.table_1.10.4 R6_2.2.1 AnnotationDbi_1.36.2 XML_3.98-1.7 ggbeeswarm_0.5.3 limma_3.30.13 reshape2_1.4.2 [36] magrittr_1.5 edgeR_3.16.5 matrixStats_0.52.2 scales_0.4.1 htmltools_0.3.6 tximport_1.2.0 assertthat_0.2.0 [43] mime_0.5 xtable_1.8-2 colorspace_1.3-2 httpuv_1.3.3 stringi_1.1.5 RCurl_1.95-4.8 lazyeval_0.2.0 [50] munsell_0.4.3 rjson_0.2.15